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Product Info Summary
| SKU: | EKC2376 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
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Product info
Product Name
c-Jun (Phospho-Ser73) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2376
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The c-Jun (Phospho-Ser73) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor c-Jun (Phospho-Ser73) protein expression profile in cells. The kit can be used for measuring the relative amounts of c-Jun (Phospho-Ser73) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on c-Jun (Phospho-Ser73).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
c-Jun (Phospho-Ser73) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2376)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2376 is reactive to JUN in Human, Mouse, Rat samples
Application Guarantee
EKC2376 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-c-Jun Phospho-Ser73 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-c-Jun Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HuvEc cells treated with TNF 20ng/ml 5' and K562 cells treated with TNF 20ng/ml 5'
Protein Target Info & Infographic
Gene/Protein Information For JUN (Source: Uniprot.Org, NCBI)
Gene Name
JUN
Full Name
Transcription factor AP-1
Weight
35676 MW
Superfamily
bZIP family
Alternative Names
Transcription factor AP-1;Activator protein 1;AP1;Proto-oncogene c-Jun;V-jun avian sarcoma virus 17 oncogene homolog;p39;JUN; JUN AP-1, AP1, c-Jun, cJUN, p39 Jun proto-oncogene, AP-1 transcription factor subunit transcription factor AP-1|Jun activation domain binding protein|activator protein 1|enhancer-binding protein AP1|jun oncogene|proto-oncogene c-Jun|proto-oncogene cJun|v-jun avian sarcoma virus 17 oncogene homolog|v-jun sarcoma virus 17 oncogene homolog
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on JUN, check out the JUN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for JUN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For c-Jun (Phospho-Ser73) Colorimetric Cell-Based ELISA Kit (EKC2376)
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