DNA repair protein XRCC2 Antibodies

DNA repair protein XRCC2 (XRCC2)

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which functions from the BRCA1-BRCA2- dependent HR pathway.

Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the junction of the four duplex arms of the Holliday junction and to junction of replication forks.

Gene Information

Human
Gene Name:	XRCC2
Uniprot:	O43543
Entrez:	7516

Family

Belongs to:
RecA family

Alternative Names

DKFZp781P0919; DNA repair protein XRCC2; X-ray repair complementing defective repair in Chinese hamster cells 2; X-ray repair cross-complementing protein 2; X-ray repair, complementing defective, repair in Chinese hamster

Molecular Weight

Mass (kDA):
31.956 kDA

Genomic Context

Human
Location:	7q36.1
Sequence:	7; NC_000007.14 (152644776..152676141, complement)

Subcellular Localizations

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Diseases

• Malignant Neoplasms
• Malignant Neoplasm Of Breast
• Neoplasms
• Mammary Neoplasms
• Cytogenetic Abnormality
• Genomic Instability
• Adenocarcinoma
• Cell Transformation, Neoplastic
• Carcinoma
• Malignant Neoplasm Of Lung
• Malignant Neoplasm Of Ovary
• Aneuploidy
• Colorectal Neoplasms

• Ovarian Neoplasm
• Fanconi Anemia
• Anemia
• Carcinogenesis
• Lung Neoplasms
• Instabilities, Chromosomal
• Colorectal Cancer

Interacting Proteins

• MEOX2
• RAD51C
• RAD51D

Pathways

• Dna Repair
• Recombinational Repair
• Double-strand Break Repair
• Cell Cycle
• Dna Replication
• Hypersensitivity
• Gene Conversion
• Mismatch Repair
• Localization
• Dna Recombination
• Telomere Maintenance
• Meiosis
• Cell Death

• Drug Resistance
• S Phase
• Neurogenesis
• Mitosis
• Cell Division
• Interstrand Cross-link Repair
• Cell Proliferation

PTMs

• Methylation

• Phosphorylation

• Deamination

Research Areas

• Breast Cancer
• Cancer
• DNA Repair
• Homologous Recombination
• Tumor Suppressors

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/XRCC2

Best Uses Of The XRCC2 Marker From Boster Bio

The XRCC2 marker, a human recombinant protein, is a DNA repair protein that is often associated with cancer. This marker can be used to study specific species or applications. In addition, it can be used to generate product credits for scientists. It is available to scientists worldwide, just as with other Boster Bio product. Let's examine the best uses of XRCC2.

XRCC2 can be used to repair DNA

XRCC2 DNA-repair Protein forms a complex, BCDX2X2 and CX3. This complex acts to recruit RAD51 onto DNA damage sites and assemble the RAD51 nuclear protein filament. Genetic studies have identified the role of XRCC2 in breast cancer susceptibility. A mutation in this gene increases the risk of developing breast carcinoma, especially in younger women, especially if there is a family history.

The XRCC2 chromosome and XRCC2 XRCC2 XRCC2 XRCC2 XRCC2 are key players in DNA damage repair and chromosome stability. Many cancers have alterations to this gene. These alterations can be found in 0.2% to 3% of all types of cancers. Most cancers involving XRCC2 have alterations in the gene. XRCC2 mutations are most common in prostate, colon, lung, and prostate adenocarcinomas.

Genotyping of XRCC2 showed that certain mutations are associated to an increased risk for bladder cancer. Specifically, rs3136817 and rs1799794 were significantly associated with bladder cancer risk. A mutated XRCC2 genes was associated with a higher risk of ovarian carcinoma. In China, polymorphisms of the XRCC2 gene were also associated with a higher risk.

XRCC2 and RAD51 are also linked to breast cancer. Both genes encode DNA repair proteins. RAD51C or RAD51D have been associated with an increased risk for breast cancer and ovarian cancer. XRCC2 was also linked with an increased chance of developing breast cancer. They are both DNA repair proteins and may contribute to breast cancer susceptibility.

While the role of XRCC2 in the correct focus formation is not yet clear, the findings suggest that it may play a role in the pathogenesis of breast cancer. The gene is found in Sri Lankan women who are postmenopausal, and is associated with a lower incidence of breast cancer. Recent research involving XRCC2 gene supports this association.

Despite the many benefits of XRCC2 for cancer susceptibility, it remains unknown if its genetic variant contributes to increased risk. However, XRCC1 has a higher risk of developing nasopharyngeal carcinoma than the XRCC2 version. Its polymorphisms have been associated to a higher likelihood of developing nasopharyngeal carcinogenesis by as much at 3.2 times. The AA genotype has a higher probability.

It is often linked to cervical cancer

A new study has shown that cervical cancer may be linked to a gene called XRCC2. The data was gathered from the two German Cancer Registries. The risk of developing cervical carcinoma was significantly higher for women who have BRCA1 and BRCA2 genetic mutations than those who come from BRCA1/2+ families. Although more research is needed to determine if XRCC2 may be linked with cervical cancer, vaccination against this virus is the best way to prevent high-risk infections.

It is a human recombinant proteins

XRCC2 is a human recombinant genetic repair gene. It can be found in many organs and tissues, including the brain. Mutations in the gene could affect the cells' ability to repair themselves. Researchers have discovered that XRCC2 can cause a variety of tissue effects in recent research. The gene may alter the ability of cells in some cancers to heal themselves.

Human XRCC2 makes up a critical component of the RAD51 Complex, a four-part compound that includes three paralogs: the CX3 complex protein and the BCDX22 protein. It is necessary for the recruitment and assembly of RAD51 at the damage site. This complex is epigenetically repressed when EZH2, a key nuclear protein involved with DNA repair, is overexpressed.

The XRCC2 marker is available from many sources. Its Uniprot ID number is O43543. It is essential to repair DNA double-strand breaks effectively and for homologous, interchromatid recombination. Mutant cells with reduced XRCC2 had a 100-fold decrease of homologous recombination. However, transient transplantation corrected this defect. Nonhomologous ending joining was also observed.

Another study showed that the XRCC2 Marker can be used to predict which clones will yield stable recombinant protein clones. The expression profiles of XRCC2 markers were used to distinguish stable clones from unstable clones. This study highlights that functional analyses of missense variants of human genes are crucial. While there is no clear association between the XRCC2Marker and breast carcinoma, it is possible for a strong association to be made between a specific mutation of a gene and the likelihood of developing a certain disease.

XRCC2 contains 303 amino compounds and was produced in E.coli. It is fused together with a 23 amino acids His-tag at N-terminus. XRCC2 should be kept at -20°C for longer storage periods. It is stable for about two weeks at roomtemperature. It should be stored in a carrier, such as 0.1% HSA/BSA. The XRCC2 solution includes 20 mM TrisHCl buffer, 0.4M Urea, and 10% glycerol.

52 cases were found to have the XRCC2 XRCC2 mutation. The hypermethylation causes a decrease in gene expression and homologous replication repair. This study showed that the gene encoding XRCC2 was hypermethylated in its promoter region. This reduced its ability to repair DNA. These results indicate that it could serve as a biomarker of the disease.