UPF3B Antibodies

Regulator of nonsense transcripts 3B (UPF3B)

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and functioning as connection between the EJC core and NMD machinery. Recruits UPF2 in the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release variables in the stalled ribosome) is believed to activate NMD.

In collaboration with UPF2 arouses both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions.

Gene Information

Human
Gene Name:	UPF3B
Uniprot:	Q9BZI7
Entrez:	65109

Family

Belongs to:
RENT3 family

Alternative Names

HUPF3B; Nonsense mRNA reducing factor 3B; regulator of nonsense transcripts 3B; RENT3BhUpf3p-X; UPF3 regulator of nonsense transcripts homolog B (yeast); UPF3XMRXS14; Up-frameshift suppressor 3 homolog B; Up-frameshift suppressor 3 homolog on chromosome X

Molecular Weight

Mass (kDA):
57.762 kDA

Genomic Context

Human
Location:	Xq24
Sequence:	X; NC_000023.11 (119805311..119853028, complement)

Tissue Specificity

Expressed in testis, uterus, prostate, heart, muscle, brain, spinal cord and placenta.

Subcellular Localizations

Nucleus. Cytoplasm. Shuttling between the nucleus and the cytoplasm.

Diseases

• Autistic Disorder
• Schizophrenia
• Impacted Tooth
• Renal Dysplasia
• Protein Truncation Abnormality
• Infectious Mononucleosis
• Developmental Delay (disorder)
• Constipation
• Macrocephaly
• Developmental Disabilities
• Child Development Disorders, Pervasive

• Dysplasia
• Congenital Heart Defects
• Speech Delay
• Schizophrenia, Childhood
• Autism Spectrum Disorders

Interacting Proteins

• EIF4A3
• MAGOH
• RBM8A
• UPF1
• UPF2

• UPF3A

Pathways

• Translation
• Localization
• Rna Surveillance
• Rna Interference
• Transport
• Cell Differentiation
• Neurogenesis
• Metaphase

• Nuclear Export
• Rna Splicing

PTMs

• Phosphorylation

• Cleavage

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/UPF3B

Boster Bio Antibodies - Best Uses Of The UPF3B Marker

Boster bio has a wide selection of antibodies against the UPF3B/RENT3B protein marker markers. Which one is best suited for your research? Learn more about gene infographics and high-affinity prima antibodies. Boster Bio's high affinity primary antibodies will be discussed in this article. Below are some of our favorite uses for each antibody.

Boster Bio Anti-UPF3B/RENT3B Antibody Picoband

This Boster Bio Anti-UPF3B/RENT3A Antibody has been created to detect UPF3B (human nonsense transcript). This antibody reacts to the UPF3B human, mouse, and rat mRNAs. Boster Bio Anti–UPF3B/RENT3B Antibody Picoband has 4 mg of trehalose and 0.9 mM of NaCl. It also contains 0.2 mg Na2HPO4. It is available as a variety of sizes and with different lengths.

Boster Bio's high-affinity primary antibodies

Boster Bio produces high quality antibodies and ELISA Kits for use in the laboratory. These reagents/antibodies are rigorously screened for performance in ELISA/WB and FC applications. Boster Bio also manufactures its own ELISA kits and antibodies, ensuring optimal efficiency and quality. The company also offers kits and antibodies for various applications, including neuroscience, cancer, and immunotherapy.

Primary antibodies are immunoglobulins that can bind to a specific antigen. A primary antibody is typically defined by its specificity and affinity, which measure how strongly it binds to its antigen of interest. Good primary antibodies are useful for detection, purification, and measurement of the antigen of interest. High-affinity antibodies are more expensive that polyclonal antibodies but are more preferred for research.

These antibodies are known to be able to withstand temperatures higher than 60°C. They are also long-lasting and heat resistant. They are also very sensitive to antigens and can be exposed to them for long periods of time. Monoclonal antibodies are more specific than primary antibodies depending on their antigens. These antibodies form a biotin-biotin connection with the antigen, which is one nature's strongest and most noncovalent bonds.

Nano-Secondaries bind to specific IgGs, and can be used in immunofluorescence. Nano-Secondaries can penetrate tissue better than conventional secondary antibodies. They also reduce the distance between epitopes (and labels) by 10x. The Nano-Secondaries can be used in immunostaining.