Netrin receptor UNC5C Antibodies

Netrin receptor UNC5C (UNC5C)

Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding.

Axon repulsion in growth cones may result from its association with DCC that may trigger signaling for repulsion. Also involved in corticospinal tract axon guidances independently of DCC.

Gene Information

Human
Gene Name:	UNC5C
Uniprot:	O95185
Entrez:	8633

Family

Belongs to:
unc-5 family

Alternative Names

netrin receptor UNC5C; Protein unc-5 homolog 3; Protein unc-5 homolog C; unc-5 homolog 3; unc-5 homolog C (C. elegans); UNC5C; UNC5H3; UNC5H3unc5 (C.elegans homolog) c

Molecular Weight

Mass (kDA):
103.146 kDA

Genomic Context

Human
Location:	4q22.3
Sequence:	4; NC_000004.12 (95162504..95549210, complement)

Tissue Specificity

Mainly expressed in brain. Also expressed in kidney. Not expressed in developing or adult lung.

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein. Cell surface. Cell junction, synapse, synaptosome. Cell projection, axon. Cell projection, dendrite. Cell projection, growth cone. Cell projection, lamellipodium. Cell projection, filopodium.

Diseases

• Malignant Neoplasms
• Neoplasms
• Colorectal Cancer
• Carcinoma
• Colorectal Neoplasms
• Nervousness
• Malignant Paraganglionic Neoplasm
• Cell Invasion
• Melanoma
• Malignant Tumor Of Colon
• Pathologic Neovascularization
• Prostatic Neoplasms
• Inflammation

• Carcinogenesis
• Cell Transformation, Neoplastic
• Injury To Kidney
• Tumor Angiogenesis
• Chromosome Breakage
• Adenocarcinoma
• Chromosomal Translocation

Interacting Proteins

• TUBB3

Pathways

• Methylation
• Axon Guidance
• Cell Migration
• Cell Proliferation
• Spermatogenesis
• Epithelial Cell Proliferation
• Dna Methylation
• Angiogenesis
• Innervation
• Cell Adhesion
• Hair Follicle Development
• Gene Silencing
• Chemokine Production

• Brain Development
• Pigmentation
• Inner Ear Development
• Wound Healing
• Melanocyte Migration
• Neuron Development
• Localization

PTMs

• Methylation
• Phosphorylation

• Demethylation
• Cleavage

• Deacetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/UNC5C

Boster Bio - Best Uses Of The UNC5C Marker

Boster is a top choice for anyone looking for a new antibody that will recognize the UNC5C Marker. Boster validates their antibodies across multiple platforms, with known positive samples and negative samples. This ensures that they are highly specific and affinity. Boster awards product credits to the first reviewer. Scientists around the globe are rewarded for their hard work. Here are some of the benefits of using this marker.

Anti-UNC5C Marker

This product is an antibody that is specific for UNC5C. It is a part of the Picoband(tm) catalog. This antibody reacts well with human, mouse, or rat cells. The Boster Bio Anti-UNC5C Marker can be stored at -20 degrees Celsius for one year. It contains 5mg Bovine Serum Albumin (BSA).

UNC5C's T835M variant causes cell death in many cell types including neurons. However, expressions of the T835M version of UNC5C result in a higher level eTau protein within the conditioned medium. This may indicate that UNC5C can be toxic if it is overexpressed. T835M is also associated with a higher death rate in many human cell lines.

The UNC5C protein is expressed in both the developing and adult brain. Its death domain is thought to influence apoptotic signaling. The T835M variant UNC5C has been validated in additional LOAD pedigrees, case/control populations, and other LOAD data. This could indicate that UNC5C might play a part in Alzheimer's. This could also have implications for other neurodegenerative diseases.

The Anti-UNC5C Marker, a monoclonal antibody against UNC5C protein, is a human monoclonal antibody. It recognizes UNC5C from the extracellular domain. Jackson ImmunoResearch used goat-antimouse biotin for the anti-UNC5C. FACScan was used as a method to analyze the cells. In addition to the primary antibody, a cell viability marker (PI) was used to gate live cells.

IHC

The UNC5C mark is a vital gene in the human genomic. This marker is not expressed by all human cells, but many of them do. This makes it necessary to have a high affinity antibody against this gene in order to detect the gene. Boster Bio manufactures this antibody and provides it to researchers. It can be used both for routine and specialized tasks. It has been validated on Western Blotting, Immunohistochemistry, and ELISA, making it one of the most reliable solutions available in the market.

ICC

The UNC5C protein, which is found on the surface of cells, is used as a fluorescent marker in immunocytochemistry. It was transiently transferred into 293Ts-derived cells. After 72 hours, the cells were washed and stained with 5 mMEDTA. This antibody recognizes UNC5C extracellular domain. Jackson ImmunoResearch used a streptavidin–APC as detection antibodies and a goat–anti-mouse immunotin antibody. After washing the samples, FACScan was used to analyze them. Furthermore, FACScan was used to analyze the samples.

Both adult and developing brain neurons contain the UNC5C protein. It contains a death domain. This domain has been suggested as influencing apoptosis-signaling. The UNC5CT835M variant is being validated in additional LOAD and case/control pedigrees. Its expression might be a risk for neurodegenerative disease.

Previous genetic studies identified several major pathways that lead to AD. These pathways include the neuroinflammation, lipid metabolism and b-amyloid cascade. However, only a few of these pathways have been shown to be genetically associated with AD. UNC5C expression is found in a large proportion of cells in human brains, including neurons in the hippocampal Pyramidal Layer. Although the UNC5C variant T835M is associated with a higher incidence of AD, it was not observed in a large Chinese cohort. In another manuscript, the frequency and UNC5C codes variants in an unconfigured exon were investigated.

Immunofluorescence

Picoband(tm); catalog contains the boster UNC5C antibodies. It reacts well with human, mouse, rat. It can be stored at -20°C for up one year. The antibody contains 5mg bovine serumalbumin.

The antibody is conjugated in high-resolution to a reporter colour to allow for the analysis of fluorescent signal. An antibody that is not conjugated to a reporter dye can only recognize unconjugated proteins. This can be problematic when there are many antigens at the cell's surface. This method eliminates nonspecific antibody binding, which can occur if secondary antibodies fail to enter cells. But what if the UNC5C antibody is used alone?

Primary antibodies

The UNC5C primer antibody marker is used for immunohistochemistry and Western Blotting to detect proteins from biological samples. This marker has high affinity and sensitivity and is widely used by researchers in the field. The antibody is guaranteed against defects for one year from the date of purchase. However, there are some precautions you should take when using this marker. Continue reading to learn about the benefits of this marker as primary antibodies.

Researchers can double-labele specimens using a primary-secondary antibody system. This allows researchers to ask more questions and obtain more contextual data. Researchers can label two specimens simultaneously with a primary-secondary antibody system. This provides better results and more robust answers. You can also combine a primary antibody with two different secondary antibodies. This process can help identify proteins and their cellular components.

It is important that you choose the right ECL kit for each primary antibody species. The kit should contain enough reagents that it covers eighty centimeters membrane. After incubating with the primary antibody, it is necessary to wash the membrane three more times with TBS Wash Buffer. A clear preservative coating should be placed on the membrane. If there are any bubbles or other blemishes, the membrane should be removed by gently blotting it with a transparent glass paper.

The UNC5C antibody in the primary antibody will detect an antigen if it is being used for immunolabeling. This protein is also known to be a monoclonal antigen. This type of antibody is highly specific, while monoclonal antibodies can be used for many purposes. These antibodies are often used in the lab to detect, purify, or measure specific antigens.