TRPV5 Antibodies

Transient receptor potential cation channel subfamily V member 5 (TRPV5)

Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine (PubMed:11549322, PubMed:18768590). Required for normal Ca(2+) reabsorption in the kidney distal convoluted tubules (By similarity).

The station is triggered by low internal calcium level and the present exhibits an inward rectification (PubMed:11549322, PubMed:18768590). A Ca(2+)-dependent feedback law includes fast channel inactivation and slow current decay (By similarity).

Gene Information

Human
Gene Name:	TRPV5
Uniprot:	Q9NQA5
Entrez:	56302

Family

Belongs to:
transient receptor (TC 1.A.4) family

Alternative Names

Calcium transport protein 2; calcium transporter 2; CAT2; ECaC; ECAC1; ECAC1CaT2; Epithelial calcium channel 1CAT2; Osm-9-like TRP channel 3; OTRPC3; transient receptor potential cation channel subfamily V member 5; transient receptor potential cation channel, subfamily V, member 5; TRPV5

Molecular Weight

Mass (kDA):
82.551 kDA

Genomic Context

Human
Location:	7q34
Sequence:	7; NC_000007.14 (142908101..142933746, complement)

Tissue Specificity

Expressed at high levels in kidney, small intestine and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum.

Subcellular Localizations

Apical cell membrane; Multi-pass membrane protein. Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells (By similarity). The expression of the glycosylated form in the cell membrane is increased in the presence of WNK3 (PubMed:18768590).

Diseases

• Hypercalciuria
• Kidney Diseases
• Hypertensive Disease
• Osteoporosis
• Hypocalcemia
• Hypercalcemia
• Kidney Calculi
• Rickets
• Malignant Neoplasms
• Pain
• Neoplasms

• Osteopenia
• Hypervitaminosis
• Hypervitaminosis D
• Calcium Metabolism Disorders
• Gitelman Syndrome

Interacting Proteins

• CALB1

Pathways

• Transport
• Excretion
• Localization
• Pathogenesis
• Aging
• Intestinal Absorption
• Bone Resorption
• Secretion
• Ion Transport
• Glycosylation
• Endocytosis
• Cell Proliferation
• Muscle Contraction

• Exocytosis
• Lactation
• Coagulation
• Reverse Transcription
• Smooth Muscle Contraction
• Cell Death
• Transepithelial Transport

PTMs

• Glycosylation
• Phosphorylation
• Cleavage
• Biotinylation

• Carboxylation
• Deglycosylation
• Acetylation
• Dephosphorylation

• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TRPV5

Best Uses Of The TRPV5 Marker

Flow procedures come with many options. Here are some optimization tips. These flow procedure optimization tips can help you get maximum value from your experiments. These optimization tips are meant to help you understand the optimization process and answer any queries you may have. These tips will help you optimize the results of your experiment.

Boster Bio has high-affinity, primary antibodies

Flowcytometry is a technique to analyze the interaction of different molecules and receptors. The sample is composed of cells and particles. Boster Bio provides primary antibodies against TRPV5 that are monoclonal or polyclonal in nature. These antibodies are highly sensitive, specific, and have been cited more than twenty-five times in scientific literature.

Primary antibodies are an important part of immunoassays. They can bind specific antigens and then measure the corresponding protein expression levels. Immunoassays include Western Blotting, immunohistochemistry, and histopathology. Other immunoassays use flow cytometry to determine a cell type. Researchers can also use antibodies as a tool to identify and quantify certain cellular components in their samples.

TRPM8 sequence amplified

TRPV5 is a protein that interacts in a variety of biological processes with TRPM8. Pull-down assays with GST-tagged M8–Nt and M8–Ct showed that both proteins were co-located with TRPM8. The TST treatment decreased co-localization by 43 + 3.8%. It is therefore useful to determine whether TST treatment reduces AR-TRPM8 interaction.

The TRPV5 gene is a novel, cation-activated transient pore protein with homology to melastatin. It is located on the chromosome 15.5 and shows allele-specific gene expression. Interestingly, the TRPV5 marker has the capacity to detect the presence of TRPV5 in a variety of cancers. These results are promising because of the many applications that the TRPV5 marker can serve.

Boster Bio is an antibody maker that specializes on picogram-level sensitivity ELISA sets. Its extensive inventory includes more that 12,000 antibodies, all of which have been validated for WB/Flow and IHC. Boster Bio antibodies have been rigorously validated against 250 different tissues. They are also quantitatively tested against untransfected cell types and recombinant proteins.

Prostate cancer has been linked to a transient potential melastatin 8 (8TRPM8) marker. TST increases, causing transient elevations in the cytosolic calcium2+ concentration. These results were independent of AR bioavailability. Moreover, the absence of AR does not alter the TRPM8 response to TST. These data support the hypothesis.

TRPM8

The TRPV5 label is an atom specific chemical structure that binds with the S1 to S4 region of TRPV5. Its binding pocket is composed of the S3 linker of one monomer and the S4-S5 helices of the other monomer. The binding pocket allows for unambiguous placements of side chains and backbones. It has a local resolution between 3.0-3.5 A. Researchers docked twelve millions compounds into the TRPV5 wallet using the Schrodinger Suite 2018-1. These compounds were taken from the ZINC15 "Drugs Now" library.

The TRPV5 monomer is identical in starting structure. It was therefore used in the development of a model protein. The Schrodinger's Receptor Grid generation tool was used to generate a docking grid centered on the lipid molecule's center in the binding pocket of monomer A. The outer grid box's dimensions were 30 A x 30 A. After the protein monomer had docked, the TRPV5 markers could be visualized with qRTPCR.

The nanodisc was pre-incubated in 3 mM F–Fos Choline eight to analyze the TRPV5 proteins. It was then double-blotted using 1.2/1.3 Quantifoil Hley Carbon grids. These grids are available from Quantifoil Micro Tools. The method for performing electron imaging is simple. It involves a liquid Ethane vitrifier with a Gatan K2 Summit electron detector. Then, a 40-frame movie was collected at a nominal dose of 40 e-/A2 and a dose rate of 5 e-/s/phys. The film was then scanned.

A 4-kb fragment was amplified via PCR to create the TRPV5 gene. The fragment was then subcloned into the PmeI site of pBS KS(+) vector. The vector also contained the neomycin-flanked site loxP. The monolayers were used for germline transmission to create chimeras.

PRL-stimulated Ca transport relies on TRPV5. Cav1.3, which is a calcium channel, is overexpressed in the absence of this ionchannel. The TRPV5 Mark is an efficient way of determining whether a protein has functional properties. This protein is important for the absorption of calcium, as it facilitates calcium transport. TRPV5 also plays an important role in many other processes in the body, such as the regulation of hormones and immune responses and bone metabolism.