TNFSF10 Antibodies

Tumor necrosis factor ligand superfamily member 10 (TNFSF10)

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and Maybe also to TNFRSF11B/OPG (PubMed:26457518, PubMed:10549288).

Induces apoptosis.

Its activity could be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG which can't induce apoptosis. .

Gene Information

Human
Gene Name:	TNFSF10
Uniprot:	P50591
Entrez:	8743

Family

Belongs to:
tumor necrosis factor family

Alternative Names

Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10

Molecular Weight

Mass (kDA):
32.509 kDA

Genomic Context

Human
Location:	3q26.31
Sequence:	3; NC_000003.12 (172505508..172523430, complement)

Tissue Specificity

Widespread; most predominant in spleen, lung and prostate.

Subcellular Localizations

Cell membrane; Single-pass type II membrane protein. Secreted. Exists both as membrane-bound and soluble form.

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Malignant Paraganglionic Neoplasm
• Leukemia
• Prostatic Neoplasms
• Malignant Neoplasm Of Prostate
• Mammary Neoplasms
• Malignant Tumor Of Colon
• Colonic Neoplasms
• Liver Carcinoma
• Malignant Neoplasm Of Breast

• Inflammation
• Liver Neoplasms
• Glioma
• Lung Neoplasms
• Adenocarcinoma
• Neoplasm Metastasis
• Melanoma

Interacting Proteins

• CUL3
• TNFRSF10A
• TNFRSF10B

Pathways

• Cell Death
• Sensitization
• Induction Of Apoptosis
• Pathogenesis
• Cell Cycle
• Cell Growth
• Cell Proliferation
• Immune Response
• Programmed Cell Death
• Secretion
• Cell Killing
• Rna Interference
• Localization

• Angiogenesis
• Cell Cycle Arrest
• Methylation
• Drug Resistance
• Cell Differentiation
• Translation
• Gene Silencing

PTMs

• Cleavage
• Phosphorylation
• Methylation
• Dephosphorylation
• Demethylation
• Ubiquitination
• Acetylation
• Reduction

• Glycosylation
• Oxidation
• Phosphorothioation
• Deacetylation
• Deglycosylation
• Sumoylation
• Nitrosylation
• Palmitoylation

Research Areas

• Apoptosis
• Cancer
• Cell Biology
• Death Receptor Signaling Pathway
• Signal Transduction

• Tumor Suppressors

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TNFSF10

Boster Bio - Best Uses Of The TNFSF10 Marker

When working in immunohistochemistry, the Anti-TRAIL/TNFSF10 Antibody Picoband(tm) is an invaluable tool. This antibody can be used for a variety of purposes, including immunohistochemistry and studies that are specific to a particular species. In this article, we will review the advantages of using the TNFSF10 marker. We will also discuss the benefits of using Boster Bio's TNFSF10 Marker for immunohistochemistry.

Anti-TRAIL/Tnfsf10 Antibody Pioband(tm).

The Anti-TRAIL/TnfsF10 Antibody Picoband(tm) produced by Boster Bio, is part of the Picoband(tm) catalog. This monoclonal antibody can be used to detect the TRAIL protein of human source. The antibody is available in non-conjugated as well as multiple-conjugated variants. Its concentration goal of 20 ng/mL is reached and it is stored at -20°C for up one year. Picoband(tm) is an anti-TRAIL antibody, has 5mg BSA (0.2mg Na2HPO4) as buffering agents. A blocking peptide may be purchased at a price that is based on the immunogen's length.

TRAIL also known as CD253 is a member of the tumor necrosis factors (TNF) superfamily. It connects to death receptors on cells and triggers the process of apoptosis. It interacts with proteins within the NFKB pathway and activates kinases. These actions aid in the detection of cancerous cells and are thus used for immunophenotyping purposes as a marker.

This monoclonal antibody is able to detect TNFSF10 in human brain tissue, embedded in paraffin sections at 4 degrees Celsius. The TRAIL/TNFSF10 antibody comes in a 0.2 um filtered solution for use in immunoblotting. The Anti-TRAIL/TNFSF10Antibody, Picoband(tm), has minimal cross-reactivity to mouse TRAIL. It was used in ELISAs using a PVDF membrane, and a HRP-conjugated second antibody.

Immunohistochemistry

The TNFSF10 marker is part of the Tumor Necrosis Factor (TNF) superfamily. It is located on chromosome 33q26 of humans and has an amino acid sequence number of 281 It is present at significant amounts in all normal tissues. Binding to TRAIL activates specific kinases and induces transcription of genes. Boster antibodies have been tested in immunofluorescence, IHC, and WB.