TNFAIP3 Antibodies

Tumor necrosis factor alpha-induced protein 3 (TNFAIP3)

Ubiquitin-editing enzyme that comprises both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens through Toll-like receptors (TLRs) through terminating NF-kappa-B activity.

Essential element of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3.

Gene Information

Human
Gene Name:	TNFAIP3
Uniprot:	P21580
Entrez:	7128

Family

Belongs to:
peptidase C64 family

Alternative Names

A20; A20TNFA1P2; EC 3.4.19.12; EC 6.3.2.-; MGC104522; MGC138687; OTU domain-containing protein 7C; OTUD7C; OTUD7CMGC138688; Putative DNA-binding protein A20; TNF alpha-induced protein 3; TNFA1P2; TNFAIP3; tumor necrosis factor alpha-induced protein 3; tumor necrosis factor inducible protein A20; tumor necrosis factor, alpha-induced protein 3; Zinc finger protein A20

Molecular Weight

Mass (kDA):
89.614 kDA

Genomic Context

Human
Location:	6q23.3
Sequence:	6; NC_000006.12 (137866317..137883312)

Subcellular Localizations

Cytoplasm. Nucleus. Lysosome.; [A20p50]: Cytoplasm.

Diseases

• Inflammation
• Arthritis
• Lymphoma
• Rheumatoid Arthritis
• Neoplasms
• Malignant Neoplasms
• Autoimmune Reaction
• Lupus Erythematosus, Systemic
• Autoimmune Diseases
• B-cell Lymphomas
• Diabetes Mellitus
• Inflammatory Response
• Psoriasis

• Autoimmunity
• Tissue Adhesions
• Leukemia
• Diabetes Mellitus, Insulin-dependent
• Carcinoma
• Marginal Zone B-cell Lymphoma
• Hodgkin Disease

Interacting Proteins

• ARRDC3
• CASP8
• IKBKG
• RNF11
• TAX1BP1

• TNIP1
• TNIP2
• TNIP3
• TRAF2
• TRIM23

• Ywhah

Pathways

• Pathogenesis
• Cell Death
• Inflammatory Response
• Immune Response
• Cell Proliferation
• Cell Activation
• Cell Adhesion
• Cell Growth
• Programmed Cell Death
• Localization
• Methylation
• Angiogenesis
• Cell Cycle

• Innate Immune Response
• Induction Of Apoptosis
• Rna Interference
• Cytokine Production
• Secretion
• Endothelial Cell Activation
• Regeneration

PTMs

• Phosphorylation
• Ubiquitination
• Cleavage

• Methylation
• Glycosylation
• Oxidation

• Acylation

Research Areas

• Cancer
• Inflammation
• Innate Immunity
• Necroptosis
• Neurodegeneration

• Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TNFAIP3

Boster Bio - Best Uses Of The TNFAIP3 Marker

Boster Bio has created a monoclonal antibody which reacts with both mouse and human TNF. TNF markers are available in both insoluble and soluble forms. It is a versatile tool that can be used by scientists and researchers. Here are some of its best uses. Continue reading for more information. You may also be interested in its applications.

Boster Bio's Anti-TNF Alpha Monoclonal antibody reacts with mouse and human TNF

Boster Bio's Anti-TNF-Alpha Monoclonal antibody reacts with mouse or human TNF, a type 2 membrane protein. It recognizes both TNFa natural and recombinant. This antibody does not cross-react with lymphotoxin. It shows background staining of connective tissues but does not cross react with lymphotoxin. The monoclonal antibody has a molecular weight of 17 kD and is 157 amino acid long. TNF-a is able to bind to surface receptors and induce a variety of biological activities including cytolysis and cytostasis in vitro and invivo of many tumor cell lines.

Activated CSF1R promotes reorganization of the cytoskeleton and regulates membrane ruffles. This protein kinase also produces proinflammatory chemokines when IL34 is activated. It plays an important part in innate immune system and regulates bone, tooth and growth. It also regulates hormone production including growth hormone. Activated CSF1R phosphorylates PLCG2, a member the protein kinase C familia. The activation and phosphorylation PLCG2 results in the activation protein kinases like MAPK1/ERK2 as well as AKT1 (phosphorylation).

Hybridomas also produce anti-TNF antibody. Hybridomas that produce monoclonal antibodies are responsible for the production of human tumor necrosis factor. Monoclonal antibodies can also be referred to as chimeric antibodies. They recognize TNF epitopes in human and mouse. A2 is used in immunoassays.

TNF-alpha is a proinflammatory cytokine that has multiple functions. It belongs to the TNF superfamily. It is a membrane-bound protein that has a theoretical molecular mass of 26 kDa. TNFalpha converting enzyme breaks down TNFalpha to produce a 157 amino-acid soluble form. The antibody recognizes TNF alpha receptors from both mice and humans.

Boster Bio's Anti-TNF-Alpha Monoclonal Antibody reacts with both human and mouse TNF. It recognizes TSK114 with its unique sequence amino acids, which is not known before. The monoclonal antibody can be used in studies of immune response and inflammation. These findings could provide new insights into the role of TNF in the body.

The antibody was created in a murine hybridoma using the TSK114 chimeric mouse/human TNF. The chimeric human TNFa hybrid mouse/human cell was derived from an immunized animal’s spleen. The hybridoma gave rise to mAbs which have specific activity against TNF in both mice and humans.

MAb11 is fluorescently labelled and is useful for intracellular membrane-bound, membrane-bound, flow cytometric and intracellular analyses of mouse TNF. It also neutralizes TNF-1a bioactivity and has multiple reports of applications. This monoclonal antibodies is useful in sandwich ELISA, ELISPOT, and other applications. It is biotinylated so it can be used as an anti-death antibody.

SPR was used to determine the kinetics for TSK114 in human TNF-a. The binding interactions of infliximab and adalimumab were determined under the same conditions as TSK114. Figure 3 shows a representative sensogram of TSK114 for human TNF-a. Table 1 lists the kinetic parameters associated with the antibodies.

TNF marker can detect a wide variety of biological phenomena

The TNFAIP3 proteins encode a multiprotein complex which plays a critical role within the inflammasome. This cellular system detects a variety pathogenic microorganisms and stressors to trigger the production of pro-inflammatory substances. GATA3, IL1R and NLRC4 marks are among these genes. These proteins are also involved in the regulation of TGFb-signaling.

The reverse transcription of RNA was performed from DC1 to DC5 after infecting with MVAB. The RT reaction mixture was adjusted to a 1:40 dilution and used for quantitative PCR. Primers and probe sets from Applied Biosystems were used to amplify genes. This marker was optimized using the TaqMan Universal PCR MasterMix.

Numerous methods for assessing the immune system have been developed through advances in immunology. These tools can be used to manage immune responses in health and disease. They can be used not only to measure cytokines, but also to analyze various biological phenomena. For example, an immune system attacking its own normal components can cause autoimmune disease. Immune tolerance can be described as the ability to distinguish self and non-self. Many autoimmune diseases have a characteristic of breaking down tolerance.

TILs are cells which express a clonal antibody. These cells are capable of evading the T-cell immune response through inhibiting the expression MHC class 1 molecules. These cells can be used in order to reactivate antitumoral immunity responses. The TNFAIP3 indicator can detect a variety biology phenomena, from inflammation and cancer.

Applications

The TNFAIP3 gene regulates a variety of cellular processes including cell survival and immune response. The presence of variants at this locus may influence the function of other genes or proteins. This study demonstrates how important this marker is for RA research. Although this marker is currently only being used in mouse models of RA, it has potential for wider applications. The study identifies several potential uses for the gene.

The TNFAIP3 gene is a highly conserved protein with 90% identity among mammalian species. It is highly expressed on T-cells (B and T-cells) and is associated to several autoimmune, autoinflammatory diseases. Its catalytic cysteine (C103) promotes the activation of NF-kb signaling. This gene has many medical applications. The TNFAIP3 marker can be used to diagnose autoimmune and inflammatory diseases.

Numerous studies have looked into the role of TNFAIP3 and psoriasis. The disease was studied in mice by using imiquimod-induced, psoriasis like dermatitis. BALB/c mice were given 5% imiquimod cream over 6 days. They were then assessed daily with modified PASI systems. Psoriasis symptoms were significantly reduced in the imiquimod-treated group.