SPAG4 Antibodies

AND SPAG4 ELISA kits, SPAG4 Proteins

Many biological assays use antibodies to detect SPAG4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with SPAG4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop SPAG4 antibodies...

We validate the specificity of these antibodies to SPAG4 by testing them on tissues known to express SPAG4 positively and negatively. Browse below to find the SPAG4 antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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SPAG4 Infographic by Boster Bio

All SPAG4 Products

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Product

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Specificity

Applications

Price

Anti-SPAG4 Mouse Monoclonal Antibody [Clone ID: OTI3E9]

Human

$523
Cat # M12546
100 µl

SPAG4 (NM_003116) Human Over-expression Lysate

Human

$960
Cat # LS006676
100 µg

SPAG4 Overview

Facts about Sperm-associated antigen 4 protein.

SPAG4 3D structure. Source: alphafold

Sperm-associated antigen 4 protein (SPAG4)

Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette.

Required for targeting of SUN3 and probably SYNE1 via a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in true posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope.

Gene Information

Human
Gene Name:	SPAG4
Uniprot:	Q9NPE6
Entrez:	6676

Family

Belongs to:

No superfamily

Alternative Names

acrosomal protein ACR55; CT127; Outer dense fiber-associated protein SPAG4; Sad1 and UNC84 domain containing 4; sperm associated antigen 4; sperm tail protein; sperm-associated antigen 4 protein; SUN domain-containing protein 4; SUN4cancer/testis antigen 127

Molecular Weight

Mass (kDA):
48.165 kDA

Genomic Context


Human

Location:	20q11.22
Sequence:	20; NC_000020.11 (35615829..35621094)

Tissue Specificity

Predominantly epressed in testis. Expressed in ejaculated spermatozoa (at protein level).

Subcellular Localizations

Membrane; Multi-pass membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, flagellum axoneme. Nucleus envelope. Nucleus inner membrane. In spermatids, it is localized in the transient manchette and in the axoneme of elongating spermatids and epididymal sperm (By similarity). Colocalized with SEPT12 at the nuclear periphery in round spermatids, at sperm neck in elongated spermatids and at midpiece regions in ejaculated spermatozoa (PubMed:25775403).

Diseases

Disease Of Adrenal Medulla
Congenital Abnormality
Malignant Neoplasms
Neoplasms
Male Infertility
Leydig Cell Hyperplasia
Hyperplasia

Infertility
Testicular Dysplasia

Pathways

Spermatogenesis
Localization
Fertilization
Cell Motility
Aging
Pachytene
Rna Interference

Meiosis
Tail Morphogenesis

PTMs

Phosphorylation

Cleavage

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SPAG4

References

PMID: 14614621 by Kennedy C., et al. Human sperm associated antigen 4 (SPAG4) is a potential cancer marker.

PMID: 9691178 by Tarnasky H., et al. A novel testis-specific gene, SPAG4, whose product interacts specifically with outer dense fiber protein ODF27, maps to human chromosome 20q11.2.