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, No Gclids
Spastin Antibodies
AND SPAST ELISA kits, Spastin Proteins
Many biological assays use antibodies to detect Spastin, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Spastin in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Spastin antibodies...
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SPAST Infographic by Boster Bio
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SPAST Overview
Facts about Spastin.
Spastin (SPAST)
ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:11809724, PubMed:15716377, PubMed:16219033, PubMed:17389232, PubMed:20530212, PubMed:22637577, PubMed:26875866). Preferentially recognizes and acts on microtubules decorated with brief polyglutamate tails: severing activity increases as the number of glutamates each tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Severing activity is not dependent on tubulin acetylation or detyrosination (PubMed:26875866). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation.
Gene Information
| Human | |
|---|---|
| Gene Name: | SPAST |
| Uniprot: | Q9UBP0 |
| Entrez: | 6683 |
Family
| Belongs to: |
|---|
| AAA ATPase family |
Alternative Names
ADPSPSpastic paraplegia 4 protein; FSP2EC 3.6.4.3; KIAA1083EC 3.6.1.3; spastic paraplegia 4 (autosomal dominant; spastin); spastin; SPG4
Molecular Weight
Mass (kDA):
67.197 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 2p22.3 |
| Sequence: | 2; NC_000002.12 (32063551..32157637) |
Tissue Specificity
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The short isoforms may predominate in brain and spinal cord.
Subcellular Localizations
Membrane; Peripheral membrane protein. Endoplasmic reticulum. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Forms an intramembrane hairpin-like structure in the membrane (PubMed:20200447). Localization to the centrosome is independent of microtubules (PubMed:15891913). Localizes to the midbody of dividing cells, and this requires CHMP1B (PubMed:18997780). Enriched in the distal axons and branches of postmitotic neurons (PubMed:15269182).; [Isoform 1]: End
Diseases
- Spastic Paraplegia, Hereditary
- Paraplegia
- Spastic Paraplegia
- Muscle Spasticity
- Neurodegenerative Disorders
- Aortic Aneurysm, Abdominal
- Paraparesis
- Paraparesis, Spastic
- Peroneal Muscular Atrophy With Pyramidal Features, Autosomal Dominant
- Weakness
- Impaired Cognition
Pathways
- Microtubule Severing
- Pathogenesis
- Transport
- Localization
- Translation
- Cytokinesis
- Abscission
- Reflex
- Cell Division
- Cognition
- Secretory Pathway
- Vesicle Transport
- Endocytosis
PTMs
- Phosphorylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SPAST
bosterbio.com/bosterbio-gene-info-cards/SPAST
References
PMID: 10610178 by Hazan J., et al. Spastin, a new AAA protein, is altered in the most frequent form of autosomal dominant spastic paraplegia.
PMID: 11809724 by Errico A., et al. Spastin, the protein mutated in autosomal dominant hereditary spastic paraplegia, is involved in microtubule dynamics.