SLITRK2 Antibodies

SLIT and NTRK-like protein 2 (SLITRK2)

It's involved in synaptogenesis and boosts excitatory synapse differentiation (PubMed:27273464, PubMed:27812321). Suppresses neurite outgrowth (By similarity).

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Gene Information

Human
Gene Name:	SLITRK2
Uniprot:	Q9H156
Entrez:	84631

Family

Belongs to:
SLITRK family

Alternative Names

CXorf2; SLITL1; SLITRK2 SLIT and NTRK-like family, member 2; SLITRK2

Molecular Weight

Mass (kDA):
95.404 kDA

Genomic Context

Human
Location:	Xq27.3
Sequence:	X; NC_000023.11 (145817829..145829856)

Tissue Specificity

Expressed predominantly in the cerebral cortex of the brain but also at low levels in the spinal cord and medulla. Also expressed in some astrocytic brain tumors such as astrocytomas, oligodendrogliomas, glioblastomas, gangliogliomas and primitive neuroectodermal tumors.

Subcellular Localizations

Membrane; Single-pass type I membrane protein. Cell membrane.

Diseases

• Schizophrenia
• Glioblastoma
• Oligodendroglioma
• Chromosome Inversion
• Brain Neoplasms
• Child Development Disorders, Pervasive
• Autistic Disorder
• Neuroectodermal Tumors
• Neoplasms
• Neuroectodermal Tumor, Primitive
• Fragile X Syndrome

• Medulloblastoma
• Autism Spectrum Disorders
• Astrocytoma
• Bipolar Disorder
• Supratentorial Primitive Neuroectodermal Tumor

PTMs

• Disulfide bond

• Glycoprotein

• Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SLITRK2

Best Uses Of The SLITRK2 Marker

The Slitrk2 biomarker is a useful assay. SLITRK2 can be found in a variety of biological samples. Antibodies have been made to detect the protein. Boster Bio makes Slitrk2 antibody for rabbit and mouse. These antibodies can be used in a variety scientific assays. The antibodies have been successful in a variety of research applications. We will now discuss the best uses the Slitrk2 antibody marker.

Boster Bio Anti-SLIT and NTRK-like protein 2 Slitrk2 Antibody

Slitrk1, and Slitrk2, are two of the most essential slit-like proteins found in the brain. Mutations in these proteins are associated with several neurological disorders, including schizophrenia and Tourette syndrome. Slitrk2 antibodies target the same domain as the protein, which is the dendrites. They can recognize HA-tagged Slitrk1 or HA-Slitrk2, allowing researchers the ability to detect Slitrk1 in a wide variety of cells, including cultured hippocampal neuron cultures and heterologous cell lines.

This Slitrk2 antibodies recognizes human Slitrk2 from cell culture supernates as well as serum and plasma. It is also effective for immunoblot analysis of HEK293T cells, which express both Slitrk1 and Slitrk2, but not Slitrk4.

The Slitrk2 family of antibodies are highly related, with significant homology to Slit protein. Slitrk2 is expressed in various neural tissues, including the ventricular layer of the brain. It inhibits neurite outgrowth in cultured neurons. The Slitrk2 antibodies has a high affinity level and specificity. They are supported by technical support programs from Sanbio, a BeNeLux distributor.

Six members make up the Slitrk family, and their mutations impact synaptogenesis. They are also associated with neuropsychiatric disorders and influence the development of different types of synapses. Transfected HEK293T cells with WT or mutant Slitrk mutants, total neurite length was measured to be DIV6.

The T418 mutation causes hydrophobic interactions to be disrupted in Slitrk1 or Slitrk2. The R32L mutant, which can be found in human Slitrk2 (and mouse Slitrk2), is not located within the major structural areas. The R32L and V89M mutations in human Slitrk2 will have minimal effect on 3D structures.

To determine the effectiveness Boster Bio Anti-SLIT, NTT-RK-like Protein 2 Slitrk2, and Boster Bio Anti-SLIT (and NTRX-RK-Like Antibody) Antibody, ANOVA was performed with post-hoc Tukey's tests. This antibody is highly effective and can be used for identifying inflammatory markers in brain.

These antibodies recognize Slitrk proteins as well as their receptors. In vitro, the recombinant IgG–PTPd antibody recognizes Slitrk2's receptor. Molecular biology experiments suggest that the inactivation mutants of Slitrk1 & Slitrk4 impairs dendritic target of neurons.

As a mouse monoclonal antibody against Slit and NTRK-like protein 2, the anti-SLIT and NTRK-like proteins 1 and 2 Slitrk2 antibodies can detect Slitrk2 with high sensitivity. To determine reproducibility, the monoclonal anti-Sigma and Covance antibodies for BL21 mice were also tested.

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Applications

Slitrks, members of the LRR family, contain tandem repeats LxxLxLxN/GxL. These proteins are essential for neuronal and cytoskeletal development, neuronal guidance, tangential nerve migration, cell adhesion, and neuronal developmental. They share high sequence homology with Robo and show no homology beyond the LRR domain. This protein is also known by its role as a Ligand for Neurexin I. Neurexin I is a pre-synaptic receptor for neurotrophins.

Slitrks are structurally similar transmembrane proteins that are predominant in the central nervous systems. These proteins are important in neuronal survival and neurite growth, as well as the development of many neuropsychiatric conditions. These proteins are also implicated in connectivity and neural signaling. However, Slitrks are still widely used in animal and human studies. We don't know much about their function.

Slitrk6 also plays a critical role in brain disease and brain development. Slitrk6 was found to be essential for the survival and outgrowth of inner ear sensory nerves in the mouse model. Mice deficient in Slitrk6 geneexpression had vestibular innervation reduced, and significant cell loss was observed in the cochlea as well as the vestibular gaglia.

Slitrks can also interact with other RTKs or neurotrophin receptors. The amino acids sequences of human TrkA (human TrkB) and TrkC (human TrkC) are similar. Neurotrophin binding to these proteins leads to receptor dimerization and activation. Phosphorylation at Y791 of Slitrk2 results in PLCg interaction with Trks.

Studies on the Slitrk2 gene revealed that schizophrenia is linked to mutations in Slitrk2. Mice deficient in Slitrk5 show reduced brain volume, and lower dendritic complexity. These findings have been further confirmed by Golgi tracing analyses. These findings show the benefits of using the Slitrk2 gene in schizophrenia. Slitrk gene mutations can also increase the risk of developing psychiatric disorders.

They are widely expressed in the mammalian CNS as well as in hematopoietic cell hematopoietic cells. They can be detected as early as embryonic day 11.5/12.5. Slitrks play a significant role in maturation of selected populations of neurons. Because they are abundant in embryonic stem cell cells, Slitrks may be involved in the development OCD spectrum disorders.