R-spondin-3 Antibodies

R-spondin-3 (Rspo3)

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis (PubMed:16543246, PubMed:21693646, PubMed:26766444). Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors which are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes.

Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6.

Gene Information

Mouse
Gene Name:	Rspo3
Uniprot:	Q2TJ95
Entrez:	72780

Family

Belongs to:
R-spondin family

Alternative Names

Cristin 1; CRISTIN1; FLJ14440; hPWTSR; hRspo3; Protein with TSP type-1 repeat; PWTSR; Roof plate-specific spondin-3; RSPO3; R-spondin 3 homolog (Xenopus laevis); RSpondin 3; R-Spondin 3; R-spondin-3; Thrombospondin type-1 domain-containing protein 2; thrombospondin, type I, domain containing 2; THSD2

Molecular Weight

Mass (kDA):
31.454 kDA

Genomic Context

Mouse
Location:	10|10 A4
Sequence:	10;

Tissue Specificity

Highly expressed in endothelial cells (PubMed:26766444).

Diseases

• Neoplasms
• Malignant Neoplasms
• Carcinoma
• Fracture
• Mammary Neoplasms
• Mammary Neoplasms, Experimental
• Cell Transformation, Neoplastic
• Leukemia
• Fracture Of First Cervical Vertebra
• Secondary Malignant Neoplasm
• Malignant Neoplasm Of Breast
• Colonic Neoplasms
• Malignant Tumor Of Colon

• Malignant Neoplasm Of Lung
• Malignant Childhood Neoplasm
• Tumor Angiogenesis
• Retroviridae Infections
• Epithelial Ovarian Cancer
• Bone Diseases
• Embryonic Mosaic

Pathways

• Endocytosis
• Clathrin-mediated Endocytosis
• Sprouting Angiogenesis
• Osteoclast Differentiation
• Gastrulation
• Cell Proliferation
• Cell Cycle Checkpoint
• Cartilage Morphogenesis
• Sex Determination
• Ossification
• Vasculogenesis
• Limb Development
• Methylation

• Stem Cell Proliferation
• Cell Cycle
• Bone Development
• Cell Growth
• Angiogenesis
• Caveolin-mediated Endocytosis
• Rna Interference

PTMs

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Rspo3

BoSTER Bio - The Best Uses Of The RSPO3 Marker

The RSPO3 gene is an important PSC modifer gene. How can you make use of this gene? Continue reading to learn more! We'll be discussing three of the most effective uses of RSPO3.

The RSPO3 gene is an important PSC modifier gene

BoSTER Bio's RNAi libraries revealed that RSPO3 is a crucial PSC moderator, and suggests it could be a candidate for PANK3 transcription factors. This gene is located within the QTL region that is home to PDE10A DHCR7, MFN2, and CCNY. The gene is also located near sscmir103-1, which is believed to regulate PANK3 transcription.

It's a PSC modifier gene

The PSC pathway is controlled by a variety of genes, including RSPO3 (Rose-Sternberg protein 3), and ESXI-1. Both are responsible for controlling Wnt-mediated signaling. The overexpression of RSPO3 in human cancers is a hallmark of translocation-induced cancers, including colon and prostate cancers. These findings highlight the need for more studies on the gene.

A single variant of the RSPO3 gene could increase the likelihood of developing PSC. However, it is not the sole cause. Other genetic determinants like HLA status are thought to have a greater impact on disease risk. It isn't known if a particular exposome will occur. Although PSC risk variants related to HLA are significant, they aren't significant in comparison to the environmental factors that are the cause of PSC risk.

In a recent study, researchers discovered that RSPO3 expression is related to the presence of PSCs. This gene is responsible for the development of an ex Vivo niche in stem cells. It is also responsible for the production of a variety of proteins that are vital to tissue regeneration and could be a target for antimicrobial medications. It has also been demonstrated that RSPO3 is involved in the progression and development of inflammatory bowel disease.

In mice, anti-RSPO3 neutralizing antibodies cause a phenotype which is consistent with the existence of a functional source for RSPO3 in the intestinal lumen. The primary sources of RSPO3 that function are the PdgfRa lineage cells within your intestine.

The study's limitations are a lack of detailed histopathological profiles or vast array of pathobiological markers. Although these limitations limit the practical application of the study the advancements in systems medicine have enabled the identification of these genetic markers. For PSC patients, rs853974 may aid in identifying a potential aggressive disease course. Its hazard ratio is 2.1 (95 percent C.I. 1.7-2.8. In the meantime, polymorphisms in RSPO3 in clinical practice are not relevant.

RSPOs are secreted polypeptides that frequently interact with Wnt genes. At present, there are four paralogous RSPO genes. The first one, RSPO1, is encoded by Xenopus. The two other proteins, RSPO2 and RSPO3, are found in humans. All three proteins have the ability to sensitize cells to the Wnt signaling pathway. RSPO2 and RSPO3 are closely associated with syndecans.

It is a crucial PSC altering gene in the process of intestinal organogenesis. RSPO3 expression promotes the formation of intestinal organoid supports and support in vitro. This makes it an important gene involved in intestinal epithelial stem cell expansion. However, RSPO1 or RSPO2 don't help to counteract the effects of RSPO3.

It has been proposed that RSPO3 regulates homeostasis of the intestinal stem cells and that RSPO3 collaborates with R-spondins as well as Wnts to regulate the growth of the crypts. Both RSPOs and Wnts are essential to maintain the intestinal crypt's homeostasis. Stromal cells are the source of the ligands that are required by intestinal stem cells. Furthermore, Foxl1 and Gremlin1 also regulate the niche of stem cells.