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- Table of Contents
Facts about Rod outer segment membrane protein 1.
.
Human | |
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Gene Name: | ROM1 |
Uniprot: | Q03395 |
Entrez: | 6094 |
Belongs to: |
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PRPH2/ROM1 family |
Rod outer segment membrane protein 1
Mass (kDA):
37.205 kDA
Human | |
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Location: | 11q12.3 |
Sequence: | 11; NC_000011.10 (62613257..62615116) |
Retina (photoreceptor). In rim region of ROS (rod outer segment) disks.
Photoreceptor inner segment membrane; Multi-pass membrane protein. Photoreceptor outer segment membrane; Multi-pass membrane protein.
In this article, we'll discuss the history of the ROM1 marker, its primary and secondary antibodies, and ELISA kits. We will also discuss Steven Boster's history and picogram level sensitivity. This is a summary of Boster Bio’s research, which focuses on developing ELISA products. And, of course, you'll find out the best uses of the ROM1 marker.
Boster Bio's antibodies include primary and secondary rabbit polyclonal antibody, which can be used for detecting a variety biomarkers in neurosciences, cancer, and inflammation. All antibodies are approved for ELISA or WB applications and Boster Bio provides a complimentary secondary antibody with every purchase. The company's product line also includes ELISA controls, kits, and solution and reagents.
Secondary antibodies are used to detect specific target antigens indirectly. Secondary antibodies are usually specific for the primary antibody's isotype and species. Secondary antibodies are often conjugated with different labels which make it easier to detect and purify. Boster Bio's ROM1-labeled antibodies are particularly useful for research and development purposes. These products are manufactured with the ROM1 marker which is a highly specific marker for molecular biology research.
Transfected cells must be processed using fluorescent immunocytochemistry to determine if an antibody reacts with the ROM1-labeled GFP. The primary antibody should be able to colocalize (or show colocalization) with the fluorescent protein. Colocalization means that both labels are located in the same spot but they are not binding to the same protein. This is important as a light microscope cannot resolve one protein in one image.
Researchers use tissue from knockouts as a primary anti-body control. This tissue is fixed in the same manner as the experimental samples. The gene that inhibits the expression of the target antigen is disabled to remove the antigen. The tissue prepared from knockout mice can be fixed in the same manner as the control sample. This method can also be used to demonstrate that primary antibodies can bind to target tissues.
Picokine(r), Quick ELISA is an upgrade of traditional ELISA protocols and delivers excellent results in less than 90 minutes. Picokine(r), Quick ELISA has both a capture antigen and a biotinylated detect antigen. The sandwich complex forms in just one step and the next step adds Avidin-Biotin-Peroxidase Complex (ABPC), a TMB substrate. The concentration of the target substance determines the blue product. PicoKine(r), Quick ELISA has been validated against a wide range of biological samples to ensure reproducibility.
Picokine(r) Quick ELISA kits feature 96-well plates with removable strips. They are ideal for researchers because they have a fast turnaround time, high sensitivity and consistency. They are compatible with a variety of biological samples and provide a 100% guarantee of a positive reaction with the target analyte. Picokine Quick ELISA Kit is a great choice for those who require a quick turnaround, high sensitivity and a simple protocol.
ELISA can be used to detect protein concentrations in liquid samples. There are three types ELISA data: a signal, no signal, and a combination of both. This can indicate low proteins or problems with the test. A difference in formats can also lead to the antigen detection antibody being overlapped. This gives a more accurate and clear picture of the protein content in the sample.
Picokine ELISA kit uses a proprietary blocking and coated technology that allows for maximum sensitivity while minimizing background. These kits are tested against biologically relevant materials, such as cell culture supernatants or blood plasma. All tests are transparently documented. To ensure consistent results, all Boster ELISA Kits are subject to rigorous quality control. Validation images and documentation are evidence of these high quality standards.
The company develops research antibodies and ELISA kits for the detection of biomarkers in cancer, neurosciences, inflammation, and developmental biology. Picogram level sensitivity are another major feature of the kits. Their immunological reagents can be purchased directly from the manufacturer or through a third-party distributor, tebu-bio. Boster Bio is an antibody manufacturer that specializes in picogram ELISA kits. Boster Bio's product range is growing every month.
Boster Bio's ELISA Kits allow researchers to detect p24 molecules at a micogram-level. This method has a low detection limit. It works by adding two times the standard deviation to the signal intensity of the controls. The linear fit of the data showed good correlation between the concentrations of p24 and signal intensity. This ELISA was linearly dose-dependent, with a correlation coefficient of 0.9954
You don't need to look far to find Steve Boster’s history. The public record listing for this person includes his current and previous addresses, known phone numbers, email addresses and more. You can also look up his relatives. If you are curious about his family history, you may perform a reverse-lookup by state. Read on to find out more about Steve Boster. Listed below are some of his most notable accomplishments.
His history began June 6, 1962. He was born in Joliet, IL, to James and Evelyn Meier. He was a former manager in sales, a member at Concordia Hall in Staunton and a lifelong soldier in the U.S. Army. In addition to his mother, he was survived by his father and 3 brothers. Steve Boster was survived by his siblings Sandra Blanton and Jack Boster. Lisa Milton and John Boster were also present. Numerous nieces, nephews, and great-grandchildren were also left behind.
PMID: 1610568 by Bascom R.A., et al. Cloning of the cDNA for a novel photoreceptor membrane protein (rom- 1) identifies a disk rim protein family implicated in human retinopathies.
PMID: 8504299 by Bascom R.A., et al. Cloning of the human and murine ROM1 genes: genomic organization and sequence conservation.