Roquin-1 Antibodies

AND RC3H1 ELISA kits, Roquin-1 Proteins

Many biological assays use antibodies to detect Roquin-1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Roquin-1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Roquin-1 antibodies...

We validate the specificity of these antibodies to Roquin-1 by testing them on tissues known to express RC3H1 positively and negatively. Browse below to find the RC3H1 antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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RC3H1 Infographic by Boster Bio

All RC3H1 Products

Product

Figures

Specificity

Applications

Price

Anti-Roquin-1 RC3H1 Antibody

Human, Mouse, Rat

ELISA, IF, IHC-P, WB

$449
Cat # A06498
0.1 mg

RC3H1 (NM_172071) Human Over-expression Lysate

Human

$960
Cat # LS149041
100 µg

RC3H1 Overview

Facts about Roquin-1.

RC3H1 3D structure. Source: alphafold

Roquin-1 (RC3H1)

Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, known as constitutive decay element (CDE), which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF, TNFRSF4 and in many more mRNAs (PubMed:25026078). Cleaves translationally inactive mRNAs harboring a stem-loop (SL), often located in their 3'-UTRs, during the first phase of inflammation in a helicase UPF1-independent way (By similarity).

This process does not involve miRNAs (By similarity). In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal centre B-cell distinction in the absence of immunization and autoimmunity (By similarity).

Gene Information

Human
Gene Name:	RC3H1
Uniprot:	Q5TC82
Entrez:	149041

Family

Belongs to:

No superfamily

Alternative Names

KIAA2025RP5-1198E17.5; RING finger and C3H zinc finger protein 1; ring finger and CCCH-type domains 1; RING finger protein 198; RNF198ring finger and CCCH-type zinc finger domains 1; roquin

Molecular Weight

Mass (kDA):
125.736 kDA

Genomic Context


Human

Location:	1q25.1
Sequence:	1; NC_000001.11 (173931084..174022357, complement)

Tissue Specificity

Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver.

Subcellular Localizations

Cytoplasm, P-body. Cytoplasmic granule. During stress, such as that induced by arsenite treatment, localizes to cytosolic stress granules (By similarity). Localization to stress granules, but not to P-bodies, depends upon the RING-type zinc finger (By similarity). ICOS repression may correlate with the localization to P-bodies, not to stress granules (By similarity).

Diseases

Autoimmunity
Autoimmune Reaction
Autoimmune Diseases
Lupus Erythematosus, Systemic
Lymphoma
Neoplasms
Diabetes Mellitus
Inflammation
Ehrlichiosis Chafeensis (diagnosis)
Immunologic Tolerance
Pancreatitis
Patient Dependence On

Malignant Neoplasms
Plague
Azoospermia
Rheumatoid Arthritis
T-cell Lymphoma
Diabetes, Autoimmune
Peripheral T-cell Lymphoma

Pathways

Localization
Translation
T Cell Homeostasis
Pathogenesis
Germinal Center Formation
Chemokine Production
Granzyme B Production
Tropism
Cell Death

Histone Acetylation
Rna Interference
Lymphocyte Activation
Secretion

PTMs

Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RC3H1

References

PMID: 23550652 by Heissmeyer V., et al. Molecular control of Tfh-cell differentiation by Roquin family proteins.

PMID: 25504471 by Schuetz A., et al. Roquin binding to target mRNAs involves a winged helix-turn-helix motif.