Prokineticin-1 Antibodies

Prokineticin-1 (PROK1)

Induces proliferation, migration and fenestration (the formation of tissue discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types.

Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma development by promoting the proliferation and migration of neuroblastoma cells.

Gene Information

Human
Gene Name:	PROK1
Uniprot:	P58294
Entrez:	84432

Family

Belongs to:
AVIT (prokineticin) family

Alternative Names

EGVEGF; EG-VEGF; EGVEGFblack mamba toxin-related protein; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1mambakine; PRK1prokineticin-1; PROK1; Prokineticin 1

Molecular Weight

Mass (kDA):
11.715 kDA

Genomic Context

Human
Location:	1p13.3
Sequence:	1; NC_000001.11 (110451149..110457358)

Tissue Specificity

Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta.

Subcellular Localizations

Secreted.

Diseases

• Tumor Angiogenesis
• Endometrial Polyp
• Neoplasms
• Malignant Neoplasms
• Pathologic Neovascularization
• Mycoplasma Pneumonia
• Hypoxia
• Carcinoma
• Ectopic Pregnancy
• Pancreatic Neoplasm
• Tissue Adhesions
• Endometriosis, Site Unspecified

• Ovarian Neoplasm
• Uterine Diseases
• Adenocarcinoma
• Malignant Paraganglionic Neoplasm
• Prostatic Neoplasms
• Fenestration (morphologic Abnormality)
• Pre-eclampsia

Pathways

• Angiogenesis
• Secretion
• Localization
• Regulation Of Angiogenesis
• Decidualization
• Transport
• Cell Adhesion
• Muscle Contraction
• Inflammatory Response
• Monocyte Activation
• Cell Proliferation
• Smooth Muscle Contraction
• Parturition

• Cell Growth
• Chemokine Production
• Immune Response
• Neurogenesis
• Protein Secretion
• Spermatogenesis
• Sensitization

PTMs

• Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PROK1

Boster Bio - Best Uses Of The PROK1 Marker

The Boster Bio guide to the best applications for the PROK1 gene is an essential read for anyone new to genetics. This guide will give you information about Boster Bio's products, how to use a validated ELISA kit and more. It also provides helpful tips regarding optimization and troubleshooting. Every researcher has had some kind of issue with their experiments at some time or another. While proper controls can eliminate many sources of error, there is no such thing as a completely foolproof solution. Using a Boster Bio troubleshooting guide can help you find the problem and solve it.

Boster Bio's product range

Antibodies can be used to determine the presence or absence of the PROK1 marker. Boster Bio offers antibodies specific for the marker. The antibodies have been tested with positive and negative samples. Furthermore they are highly specific and possess a high affinity. Boster Bio products are also backed by a Boster Guarantee. The guarantee covers the products' performance in the above applications.

Validated ELISA Kits

PROK1 is a human-coded protein that is encoded by the human genome. It is located on chromosome 1 and has the predicted mature protein length of 86 amino acids. It is responsible for encouraging growth, migration, and membrane discontinuities within capillary epithelial cell membranes. PROK1 is usually used in combination with VEGF, the vascular endothelial development factor.

This kit is used to measure levels of Human Prokineticin 1 serum and plasma. The kit contains 5 mg BSA in each vial. The reagents are stored at -20°C or 4°C. To facilitate the testing of multiple biological samples the kit is available in five kits. Boster Bio's anti-ProK1 antibody is extremely sensitive and exhibits excellent linearity.

Techniques

The PROK1 gene regulates a variety of pathways. If activated, it can promote the proliferation of cells and angiogenesis. It is also involved in activation of matrix metalloproteinases including MMPs 2 , 7 and 2.

The expression of PROK1 is regulated in endometrial tissues and in pigs. The expression of PROK1 is highest in the human uterus at eight to eleven weeks gestation, and gradually decreases in the first trimester. Impaired PROK1 signaling has been linked to pathologies of the placenta. Here are the methods used to use the PROK1 marker to detect abnormalities in laboratory animals. These techniques will allow researchers to better understand the role this protein plays in the early stages of pregnancy.

PROK1 also affects many genes related to pregnancy. In trophoblasts it stimulates the expression of genes linked to angiogenesis as well as the immunological response. It is responsible for the phosphorylation MAPKs which is a crucial protein in the formation of the development of a pregnancy. The PROK1 gene is vital in the initial stages. However, it's not well understood in the trophoblasts. In porcine trophoblaststhe the levels of PROK1 are high during early placentation and during implantation. The PROK1 gene expression profile was found in porcinetrophoblasts. It regulates the expression and function of several genes that are vital in angiogenesis, immunological reactions, and adhesion of trophoblasts to each other.

To better understand the function of PROK1 in the fight against cancer, it is necessary to study how high levels of PROK1 protein are associated with the risk of developing cancer. Studies of tumors with high levels of PROK1 in the tumor's microenvironment have been proven to predict a lower chance of survival, indicating the importance of cancer-related PROK1 expression in the clinic. PROK1 expression may help in the selection of clinical trials for cancers.