Phf5a Antibodies

AND Phf5a ELISA kits, Phf5a Proteins

Many biological assays use antibodies to detect Phf5a, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Phf5a in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Phf5a antibodies...

We validate the specificity of these antibodies to Phf5a by testing them on tissues known to express Phf5a positively and negatively. Browse below to find the Phf5a antibody that suites your experiment. We have 1 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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Phf5a Infographic by Boster Bio

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PHF5A (NM_032758) Human Over-expression Lysate

Human

$628
Cat # LS084844
100 µg

Phf5a Overview

Facts about PHD finger-like domain-containing protein 5A.

PHD finger-like domain-containing protein 5A (Phf5a)

Required for maintenance of ESCs self-renewal and cellular reprogramming of stem cells. Maintains pluripotency by recruitment and stabilizing PAF1C on pluripotency genes loci, and by modulating the expression of the pluripotency genes.

Regulates the deposition of elongation-associated histone modifications, such as dimethylated histone H3'Lys-79' (H3K79me2) and trimethylated histone H3'Lys-36' (H3K36me3), on PAF1C targets, self-renewal and pluripotency genes. Regulates RNA polymerase II promoter-proximal pause release of the PAF1C targets and self- renewal genes, and the degrees of elongating ('Ser-2' phosphorylated) RNA polymerase II in their gene bodies.

Gene Information

Mouse
Gene Name:	Phf5a
Uniprot:	P83870
Entrez:	68479

Family

Belongs to:

PHF5 family

Alternative Names

bK223H9.2; INI; MGC1346; PHD finger protein 5A; PHD finger-like domain protein 5A; PHD finger-like domain-containing protein 5A; PHD-finger 5a; Rds3; SAP14b; SF3b14bSplicing factor 3B-associated 14 kDa protein; splicing factor 3B associated 14 kDa protein

Molecular Weight

Mass (kDA):
12.405 kDA

Genomic Context


Mouse

Location:	15\|15 E1
Sequence:	15;

Tissue Specificity

Expressed in primary spermatocytes (at protein level) (PubMed:18758164). Ubiquitously expressed in pre- and postnatal tissues (PubMed:12054543). Highly expressed in pluripotent embryonic stem cells (ESCs) (at protein level) and induced pluripotent stem cells (iPSCs) (PubMed:27749823).

Diseases

Malignant Neoplasms
Malignant Tumor Of Cervix
Trematode Infections

Pathways

Localization
Spermatogenesis

PTMs

Acetylation

Phosphoprotein

Research Areas

Core ESC-Like Genes
Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Phf5a

References

PMID: 12054543 by Trappe R., et al. Identification and characterization of a novel murine multigene family containing a PHD-finger-like motif.

PMID: 18758164 by Rzymski T., et al. PHF5A represents a bridge protein between splicing proteins and ATP- dependent helicases and is differentially expressed during mouse spermatogenesis.