PCNA-associated factor Antibodies

AND PCLAF ELISA kits, PCNA-associated factor Proteins

Many biological assays use antibodies to detect PCNA-associated factor, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with PCNA-associated factor in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop PCNA-associated factor antibodies...

We validate the specificity of these antibodies to PCNA-associated factor by testing them on tissues known to express PCLAF positively and negatively. Browse below to find the PCLAF antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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PCLAF Infographic by Boster Bio

All PCLAF Products

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Specificity

Applications

Price

Human KIAA Recombinant Protein

SDS-PAGE

$266
Cat # PROTQ15004
Starting from 10ug

PCLAF (NM_014736) Human Over-expression Lysate

Human

$628
Cat # LS009768
100 µg

PCLAF Overview

Facts about PCNA-associated factor.

PCLAF 3D structure. Source: alphafold

PCNA-associated factor (PCLAF)

PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions.

Also acts as a regulator of centrosome number. .

Gene Information

Human
Gene Name:	PCLAF
Uniprot:	Q15004
Entrez:	9768

Family

Belongs to:

No superfamily

Alternative Names

HCV NS5A-transactivated protein 9; Hepatitis C virus NS5A-transactivated protein 9; KIAA0101; NS5ATP9p15PAF; OEATC-1OEATC1; Overexpressed in anaplastic thyroid carcinoma 1; p15(PAF); PAF; PCNA-associated factor

Molecular Weight

Mass (kDA):
11.986 kDA

Genomic Context


Human

Location:	15q22.31
Sequence:	15; NC_000015.10 (64364304..64387687, complement)

Tissue Specificity

Expressed predominantly in liver, pancreas and placenta. Not detected in heart or brain. Highly expressed in a number of tumors, especially esophageal tumors, in anaplastic thyroid carcinomas, adrenocortical carcinomas, and in non-small- cell lung cancer lines.

Subcellular Localizations

Nucleus. Cytoplasm, perinuclear region. Following DNA damage, localizes to DNA damage sites (PubMed:21628590). Colocalizes with centrosomes in perinuclear region (PubMed:21673012).

Diseases

Neoplasms
Malignant Neoplasms
Carcinoma
Cell Invasion
Liver Carcinoma
Mammary Neoplasms
Malignant Paraganglionic Neoplasm
Liver Neoplasms
Cell Transformation, Neoplastic
Malignant Neoplasm Of Breast
Non-small Cell Lung Carcinoma
Colorectal Neoplasms
Tumor Progression

Malignant Neoplasm Of Lung
Carcinogenesis
Lung Neoplasms
Malignant Neoplasm Of Stomach
Hyperplasia
Stomach Neoplasms
Neoplasm Metastasis

Interacting Proteins

PCNA

Pathways

Cell Cycle
Dna Repair
Dna Replication
Cell Growth
Cell Proliferation
Cell Death
S Phase
Mitosis
Methylation
Pathogenesis
Gene Silencing

G1 Phase
Localization
Cell Division
Translation
Regulation Of Dna Repair

PTMs

Methylation

Acetylation

Research Areas

Cancer

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PCLAF

References

PMID: 15334068 by Petroziello J., et al. Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer.

PMID: 22096502 by Jain M., et al. KIAA0101 is overexpressed, and promotes growth and invasion in adrenal cancer.