Pannexin-2 Antibodies

Pannexin-2 (PANX2)

Structural part of the gap junctions and the hemichannels. .

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Gene Information

Human
Gene Name:	PANX2
Uniprot:	Q96RD6
Entrez:	56666

Family

Belongs to:
pannexin family

Alternative Names

hPANX2; MGC119432; pannexin 2; Pannexin2; Pannexin-2; PANX2; PX2

Molecular Weight

Mass (kDA):
74.447 kDA

Genomic Context

Human
Location:	22q13.33
Sequence:	22; NC_000022.11 (50170731..50180295)

Subcellular Localizations

Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.

Diseases

• Neoplasms
• Glioma
• Malignant Neoplasms
• Ischemia
• Malignant Paraganglionic Neoplasm
• Epilepsy
• Neoplasm Metastasis
• Brain Ischemia
• Stenosis
• Neurodegenerative Disorders
• Malignant Neoplasm Of Brain

• Confusion
• Epithelioid Sarcoma
• Sarcoma
• Carcinoma
• Nervousness
• Cerebrovascular Accident

Pathways

• Localization
• Transport
• Cell Proliferation
• Cell Death
• Cellular Localization
• Cell Communication
• Apoptotic Cell Clearance
• Sensory Processing
• Glycosylation
• Neurogenesis
• Cell Motility

• Intercellular Transport
• Bone Development
• Vasoconstriction
• Pathogenesis
• Regulation Of Blood Pressure

PTMs

• Biotinylation

• Glycosylation

• Palmitoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PANX2

Best Uses Of The PANX2 Marker

The PANX2 marker can be used for a variety of different applications. Researchers can use it for testing an application or to study a species. Scientists can submit results and receive product credit for their work. This option is open to all scientists worldwide. However, there are some key considerations to make when choosing a PANX2 marker. You can read on to learn more about the benefits this versatile marker offers.

Primary antibodies of high-affinity

Boster Bio offers high quality rabbit polyclonal antibodies and validated ELISA kits (IHC, WB. FC, ELISA) among the many types of antibodies. Boster Bio also offers rabbit monoclonal antibodies and a second antibody for free when you purchase a primary antibody. Boster Bio produces all antibodies using high affinity rabbit polyclonal antibodies. It also maintains biological activity by avoiding cross reaction.

The principle behind enzyme–linked immunosorbent tests (ELISAs), is similar to those of other immunoassay techniques. They use specific antibodies to bind the target antigen, and a detection device to indicate binding. High-affinity antibodies must be coated on a plate to maximize ELISA sensitivity. Boster Bio is a world leader in coating plates using these antibodies. Boster Bio high affinity primary antibodies are trusted by scientists worldwide, ensuring high-quality outcomes.

Research can be made more efficient by using flow cytometry antibodies. The ability to identify proteins of high affinity quickly means that you don’t need to resort to expensive and time-consuming methods. Flowcytometry is a powerful tool for identifying specific proteins. It allows for more detailed analysis. Boster high affinity antibodies can be purchased in monoclonal, as well as polyclonal, forms. Boster also has online tutorials and troubleshooting guides for immunoassays.

In a recent study, humanized variable bits, also known under the name h5E12scFv or h5E12scFv was created by grafting CDRs for murine m5E12 onto antibody chains. These chains were subjected to back-mutation and the N97 residue of the heavy chain was mutated into conserved residues. The resulting antigens show high affinity to human PCSK9.

A highly-affinity hybridoma derived mAb against the hPCSK9 was created for therapeutic applications. To reduce the immunogenicity of selected murine mAbs, the humanization process reduced the number of CDRs. The murine FRs of selected murine mAbs were then back-mutated using alanine scanner mutational analysis as well as saturated site-directed mutagenesis. The optimized scFvs were then restructured into a full-length IgG, and fusionbed to a human IgG1 constant area.

Excellent control

PANX2 can be described as a brain-derived molecule which participates in neurogenesis. PANX2 participates in these functions and has been implicated in multiple molecular pathways such as immune infiltration. This study evaluated the role played by PANX2 in LGG progression. In addition to its role in glioma progression, PANX2 has been found to be involved in a wide range of tumor types, from non-invasive benign lesions to metastatic melanoma.

To generate PANX2KO Rek cells, we used Synthego's nucleofection protocol CRISPR protocol. This kit targets Exon 2 of the Rat Panx2 gene. The nucleofection protocol also includes a complexed Ribonucleoprotein (rRNA), which targets the PANX2 Gene. These experiments were performed on two cells: one with PANX2 and one with b–actin expression as a loading test.

The length and sequence of PANX2 variants mouse and human are different. PANX2 mRNA may differ in length due to differences between sequencing and cloning. The mouse Panx2-202 gene is reported in Ensembl but not in National Center for Biotechnology Information. This could indicate tissue-specific PANX2 protein expression. Additionally, PANX2-202 could exhibit distinct subcellular localization, channel function, or inter-action with its binding partners. It is not clear what the functional implications of PANX2's mRNA and its associated RNA are.

Using a TIMER analyze, it was found that the levels and expression of the Panx2 gene in LGG cell lines was related to immune infiltration. TIMER analysis also revealed negative correlations between PANX2 expression and immune cells. This was true for PANX2 and CD4+ T cells. These data suggest that the Panx protein family may play an important role in the progression LGG.

Education

Originally, it was believed that the PANX2 marker would be found in ERs. However CLEM data has shown that it is more commonly found in intracellular vesicles. Endosomes organize cells. These vesicles transport protein from the plasma membrane, Golgi apparatus, and lysosomes to different cellular compartments. They are classified as recycling endosomes, early, late and recycling.

Research requires suitability

Panx2-WT's Panx2-WT protein has a diffuse cytoplasmic distribution. Figure 6A merged images shows overlap with other organellar or cellular markers. The overlap with Clathrin (10-19%) was significant but not as high as that with Panx2 (60%) nor p47A signals (p47A). This high percentage of overlap was consistent with localization to early endosomes.

In this study, we found that the progression of human carcinomas is associated with the pannexin proteins. Poor survival in renal cell carcinoma (RCC) is associated with the pannexin protein PANX2. In addition, PANX2 promotes the proliferation of RCC cells, making it a valuable biomarker for prognosis in PCa. However, there are few studies that address the clinical utility of the PANX2 marker for research.