N-glycosylase/DNA lyase Antibodies

N-glycosylase/DNA lyase (OGG1)

DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA.

Has a beta- lyase activity that nicks DNA 3' to the lesion. .

Gene Information

Human
Gene Name:	OGG1
Uniprot:	O15527
Entrez:	4968

Family

Belongs to:
type-1 OGG1 family

Alternative Names

8-hydroxyguanine DNA glycosylase; 8-oxoguanine DNA glycosylase; AP lyase; DNA-apurinic or apyrimidinic site lyase; HOGG1; MMH; MUTMOGH1HMMH; N-glycosylase/DNA lyase; OGG1

Molecular Weight

Mass (kDA):
38.782 kDA

Genomic Context

Human
Location:	3p25.3
Sequence:	3; NC_000003.12 (9749944..9788246)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Nucleus, nucleoplasm. Nucleus speckle. Nucleus matrix. Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.; [Isoform 1A]: Nucleus.; [Isoform 2A]: Mitochondrion.

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinogenesis
• Malignant Neoplasm Of Lung
• Lung Neoplasms
• Carcinoma
• Cell Transformation, Neoplastic
• Adenocarcinoma
• Inflammation
• Colorectal Cancer
• Malignant Squamous Cell Neoplasm
• Neurodegenerative Disorders

• Colorectal Neoplasms
• Malignant Paraganglionic Neoplasm
• Malignant Neoplasm Of Breast
• Adenoma
• Hepatocarcinogenesis
• Liver Neoplasms
• Malignant Tumor Of Colon

Pathways

• Dna Repair
• Aging
• Pathogenesis
• Localization
• Mismatch Repair
• Cell Death
• Cell Proliferation
• Base-excision Repair
• Cell Cycle
• Dna Replication
• Excretion
• Cell Growth
• Transport

• Methylation
• Senescence
• Inflammatory Response
• Response To Oxidative Stress
• Electron Transport
• Mitochondrial Dna Repair
• Cell Differentiation

PTMs

• Cleavage
• Oxidation
• Phosphorylation
• Methylation
• Acetylation
• Biotinylation

• Reduction
• Demethylation
• Nitration
• Deamination
• Deacetylation
• Ubiquitination

Research Areas

• Base Excision Repair
• Cancer
• Chromatin Research
• DNA Repair

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/OGG1

Boster Bio: Best Uses Of The GMP1 Marker

Boster Bio, Best Uses For The GMP1Marker is an excellent choice to gene analyze for scientists. It is a versatile and useful marker that can be used in many areas of science. Boster scientists have the ability to submit their results for species and application studies. They can also get product credits for their work. These benefits are available to scientists from all countries. These are just some benefits of this gene-expression assay.

Genes

Ogg1 is a gene that is expressed in human bronchial epithelial cell cells. Its expression was correlated with NOS by immunofluorescence and Pearson correlation coefficients. Interestingly, the expression of Ogg1 was downregulated in NOS-deficient cells. These results show that Ogg1 is closely associated with NOS signaling. However, there are some caveats when it comes to Ogg1 and NOS signaling.

Ogg1 is a gene that detects sequences guanine rich. The gene encodes an enzyme which detects 8-oxoG and excises this mutagenic base product. This enzyme is essential in determining whether a cell has an OGG1 mutant. The gene is therefore present in all organs.

Clinical applications

In the second half of the study, 925 individuals who had a genetic background for OGG1 were included in a mutational evaluation of the gene. There were 15 mutations in the OGG1 gene that were identified, including intronic, 3'UTR and nonsense mutations. These mutations were associated to a higher risk of developing breast Cancer.

The results suggest that a germline OGG1 OGG1 Polymorphism causing an missense mutation (Ser326Cys), may have a significant impact upon survival. A significant proportion of the population harbors the variant allele. However, it is not clear what the clinical significance of the variant gene for survival might be. However, it is important for patients to be aware that patients with variant alleles have a lower chance of surviving. The OGG1 polymorphism in the N-RCT is associated with slightly increased genotoxicity. The polymorphism was seen in peripheral lesions that had been exposed to only a fraction (3 Gy) of radiation.

Lung cancer is also linked to OGG1. OGG1 expression may be reduced, which can lead to mutagenesis or carcinogenesis. The following table lists cancers that are associated to reduced OGG1 transcription. This marker is currently being studied to determine its clinical utility. In addition to lung cancer, OGG1 is also a useful marker for identifying cancers associated with oxidative stress. These are the LOH regions most commonly found in lung cancer.

Polymorphisms

OGG1 gene polymorphisms affect the protein's ability to bind DNA. Mutations in A288V or A53T caused a decrease in the protein's catalytic activities. However, the enzyme's functions are not affected by these polymorphisms. They may increase your risk of developing gastrointestinal cancers or prostate cancer.

Experiments with cells expressing OGG1 proteins were done to study the interaction between polymorphisms, the expression of OGG1 genes, and cell proliferation. The cells were then exposed for 7 days to DNA-damaging agents such as H2O2 (fluorescent protein GFP). Seven days later, colonies were counted. The polymorphic OGG1 protein significantly reduced colony formation compared to the wild type.

The polymorphism in OGG1 (Cys326) is associated with increased risk for BC. The oxidizing conditions that can cause BC could be due to internal cellular processes as well as external radiation exposure. In a recent study, Synowiec et al. studied the association between genetic polymorphisms and BC. OGG1's Cys variant protects against breast-cancer development.

DDB2 might also be required for 8 oxoG recognition within certain regions. DDB2's absence reduces OGG1's recruitment to the occluded regions. DNA polymerase b is required to recognize it. This mechanism has a number of implications and is the subject of further research.

Chromatin immunoprecipitation assays

OGG1 is a DNA sequence implicated in negative regulation of programmed death. We performed a chromatin immunoprecipitation test using MCF-10A cells to confirm its presence in DNA. The chromatin was collected using a USB Corporation ChIP Kit and treated with E2 (10nM), VitC (1 mM) and BHA (250mM). The cells were then crosslinked with 1% formaldehyde and the soluble chromatin was extracted. After incubation for 30 min, a goat polyclonal antibodies against NRF2 were added to the cells.

The antibodies used in ChIP assays are contained in the Magna ChIP(tm). HiSens kit. It contains 10 uLs of purified IgG, and follows a recommended protocol. The product number 17-10460 was used to conduct the ChIP experiments. The cells were fixed before the ChIP experiment. The kits include a high sensitive method that identifies OGG1-expressing chromatin areas.

OGG1 gene is very conserved and has no known homologs. OGG1 is found in all tissues, including lung cancer cells and mammary tissue. It plays an important part in DNA base repair, mutagenesis, and other functions. Researchers can use chromatin immunoprecipitation tests using the OGG1 marker to determine the role OGG1 plays in the immune response as well as in the development of various types of diseases.

Nos

OGG1 is a crucial regulator for cell survival post-IR. Ogg1 enhances DNA damage site processing and DNA resistance to hydrogen peroxide. Lack of OGG1 results in an increased level of oxidative damage and a reduced cell survival after IR. Further research is needed to determine the relationship between NOS signaling activity and BER activity. The OGG1 gene plays a key role in regulating oxidative and reactive stress.

The gene is involved the BER pathway which is a major DNA-repair pathway that coordinates the repair of oxidative base damage. This pathway prevents the accumulation of premutagenic lesion, which helps to maintain genomic stability. Ogg1 plays a special role in the repair of lesions containing 8-oxoguanine. This type oesion promotes GC/TA conversions.

Numerous studies have examined OGG1's role. The marker has been shown in vitro to modulate DNA glycosylase/lyases activity. In vitro, NEDD4L has been shown to ubiquitylate OGG1 genes. The gene expression of this protein depends on NEDD4L. To determine the function of gene expression modulation, a plasmid containing OGG1 in various mutations can be analysed.

Cells expressing K249Q or C253A showed an increase in activation of the OGG1 genes. IIR is dependent on the binding of the OGG1 genes to the DNA template. Activation of the OGG1 gene through OGG1 binding is associated with ROS signaling. It has been shown, however, that OS is not related to the activation OGG1-induced Cytokines.

Cys polymorphism

Antibodies are used as markers to detect Interleukin-1alpha. These antibodies are monoclonal as well as polyclonal. They react with specific proteins from different animal species. These antibodies are developed by Boster Bio using rabbit and mouse. IL-1 stimulates THYMOCYTE ProLIFERATION, INDUCES IL-2 REELEASE, and BELL CELL MATURATION AND PROLIFERENCE.

Researchers found that homozygous individuals harboring the Cys19Arg polymorphism in the Boster bio gene had higher levels of VLDL-C and TG than did subjects with genotypes of Gly16Arg or Gln27Glu. However, statistical significance was not achieved by this finding. Despite the lack of significant associations, the findings suggest that this genetic variation may play a role in the development of inflammatory diseases, such as Alzheimer's.

The absence of hydrophobic segments within the precursor sequence of IL-1 suggests that the protein is secreted by damaged cells. This hydrophobic segment might be absent due to a different secretion process than IL-1. Boster Bio, a leader in the development of picogram sensitive ELISA kit and research antibodies, is still available. Their antibody repertoire covers 250 cell lines and tissues and has been thoroughly validated for flow cytometry and immunofluorescence.