NOVA1 Antibodies

RNA-binding protein Nova-1 (NOVA1)

May modulate RNA splicing or metabolism in a specific subset of developing neurons. .

Gene Information

Human
Gene Name:	NOVA1
Uniprot:	P51513
Entrez:	4857

Family

Belongs to:
No superfamily

Alternative Names

neuro-oncological ventral antigen 1Nova-1; Onconeural ventral antigen 1; Paraneoplastic Ri antigen; RNA-binding protein Nova-1; Ventral neuron-specific protein 1

Molecular Weight

Mass (kDA):
51.727 kDA

Genomic Context

Human
Location:	14q12
Sequence:	14; NC_000014.9 (26443090..26598033, complement)

Subcellular Localizations

Nucleus.

Diseases

• Autoimmune Reaction
• Neoplasms
• Autoimmune Diseases
• Nervousness
• Nerve Degeneration
• Paraneoplastic Syndromes
• Obesity
• Autoimmunity
• Instabilities, Chromosomal
• Ataxia
• Polyps
• Glioma
• Aneuploidy

• Malignant Neoplasms
• Paroxysmal Nocturnal Dyspnea
• Chromosome Breakage
• Acute Interstitial Pneumonia
• Dysequilibrium Syndrome
• Stress, Psychological
• Hepatitis

Interacting Proteins

• GABRG2

Pathways

• Mrna Splicing
• Translation
• Gene Silencing
• Dna Repair
• Immune Response
• Synaptic Transmission
• Muscle Contraction

• Interphase

PTMs

• Phosphorylation

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NOVA1

Best Uses Of The NOVA1 Marker

You have come to the right spot if you are looking for the best NOVA1 markers antibodies, ELISA kits and other products. Boster Bio is a manufacturer of antibodies to biomarkers in neuroblastoma (inflammation), and developmental biology. These antibodies and ELISA kits are sensitive to levels as low as picograms. They are also available through Boster Bio’s subsidiary, Tebu-bio.

NOVA1 Marker in neuroblastoma

The NOVA1 marker has emerged as a vital biomarker for neuroblastoma. NOVA proteins bind RNA using sequence-specific motif UCAUY. Nova-1's target sequence (GlyRa2) is pre-mRNA. This protein is also capable to recognize splice donors, thereby promoting the inclusion of exons. This study provides an understanding of the biological functions of Nova1 in neuroblastoma.

In this study, we discovered a gene called NOVA1 to control the expression of a protein and a growth hormone. The expression of this protein is determined using immunohistochemical staining. The tumor cells showed high expression of the NOVA1 marker. Neuroblastoma is one of the cell types that express this protein. The gene-expression level of a variety cancers controls the gene's expression.

NOVA1 is also expressed in stromal cells, immune cells, and the tumor microenvironment. This gene was found to be associated with tumor cells, T lymphocytes and stromal spindle cell expression. However, low NOVA1 expression was associated with downregulation of CD8+ T cell-related genes, such as TWIST. The miR146 regulates the expression of the protein.

Antibodies against NOVA protein were raised against anti–T7 antibody, which recognizes the entire Nova-related antigens. These antibodies recognize both the 52 kDa and larger bands. Nova-2-specific, peptide antibodies recognize additional 52–55 kDa strands in the hindbrains from wild-type animals. The same antisera fail in Nova-1 heterozygous/null mice.

ELISA kits

There are many places where you can purchase ELISA kits for the NOVA1 marker. These kits are generally available in 96-well format, but can be modified for use on 384-well plates. These kits require very little to no hands-on experience. They can also be used to accurately determine how much antigen is in a sample. The ELISA assay is sensitive to the amount of antigen present in the sample. Temporary readouts should be obtained within a few minutes. Information derived from a single test is based on the amount and time taken to obtain the results.

The NOVA1 marker ELISAkit is designed for the detection and quantification of the protein NOVA1. The NOVA1 protein is present in both human and animal cells. The antibody binding to NOVA1 protein in a patient sample is approximately 200 ng/ml. Although NOVA1 can be found in many people's skin, only a handful of cases have been identified. Researchers will appreciate a sandwich ELISA.

Competitive ELISA kits use a labeled antibody in place of an antigen, which reduces the binding of the antigen to the antibody. These kits produce more sensitive results because the amount labeled antigen present in a sample is greater than the antibody bound. This ELISA assay has many advantages, including a shorter protocol that saves time and reagents.

NOVA1 is a multifunctional protein and has various biological functions. It is found in many brain tissues and is responsible for controlling the glycine-receptor alpha-2 chain. It is found in the brain and other types of cells. NOVA1 can also be called heterogeneous nuclear-ribonucleoprotein (HNRNPK) and is a 463-amino acid protein with a 50,976 dalton mass. It has been linked to Au-Kline syndrome.

ELISA kits are available in sandwich or multiwell formats for the NOVA1 protein. Both multiwell formats and sandwich formats provide specific and sensitive results. Sandwich ELISA kits that detect the NOVA1 mark are compatible with a wide array of samples. However, sandwich ELISA kits require two specific antibodies. They must be different. The use the same antibody for detection and capture can reduce dynamic range and sensitivity.

Primary antibodies

Primary antibodies are immune globulins which bind to certain antigens. Two criteria are used to measure them: affinity and specificity. The first measures how strong the non-covalent link between the antibody & the antigen is. The latter shows how many unintended and unwanted antigens the antibody recognizes. Good primary antibodies are useful in detecting and measuring the antigen, as well as purifying and analysing it.

Steven Boster was the inventor of his first product, an IHC-kit, in the late nineteen90s. Boster wanted to be the largest catalog antibody manufacturer in the world by the late 1990s. To further enhance their product range, Boster Bio introduced its patented ELISA platform PicoKine(tm). This platform is based exclusively on trade secrets and provides high-sensitivity ELISA kits.

Biotinylation is a site-specific process that improves the labeling and labeling of antibodies. Photo-inducible unnatural amino acid (Z Biotin) can be added to primary antibodies to improve their labeling ability. This technology can be used for immunoassays as well as other related applications. The Boster Bio Primary antibodies using the NOVA1 marker are produced exclusively from human plasma and are available in a wide range of molecular weights.

These antibodies detect the same antigen in human and mouse tumours. In addition, they recognize the same antigens on both autologous and heterologous cells. These results have implications for immunotherapy as well as diagnostic imaging. These antibodies could help improve patient outcomes. And because this antibody is based on multiple methods of validation, the NOVA1 marker is a good choice for this application.

The conjugated mAbs are excellent secondary antibodies for the detection of certain avian and mammalian infections. They do not react with mammalian igGlins, which reduces background staining for mouse detection systems. NOVA1 antibodies also recognize specific IgGY antibodies, such as those to Newcastle disease virus. This allows for the more specific detection IgA/IgM.

Boster scientists can submit their results for species or special samples to get product credits. They can also submit their results to be eligible for product credits. Scientists all over the globe can apply for product credit. Boster's website has more information on NOVA1 antibody research. There are many resources that will help you find the best antibodies for your research. This marker can be used in your laboratory to enhance the sensitivity.

RNA-binding protein

In a broader context, RNA-binding protein 43 (RBFOX3) is an important factor that regulates alternative splicing of pre-mRNA. RBFOX3 regulates neuronal growth and function. Researchers have used antibodies to detect this protein in a variety samples from animals. Boster Bio uses rabbit and mouse to produce its antibodies.

RBFOX3 is a member of the Fox family of alternative splicing regulators. The RBFOX3 gene encodes a protein with a unique RNA binding site, which allows researchers to study the expression of this protein in neurons. Researchers can determine the expression levels of RBFOX3 within neurons by using an anti-RBFOX3 antibody. Antibodies against RBFOX3 recognize the R3H domain which normalizes the protein’s expression in Thymus, Neuronal tissues, and Thymus.