NFATC4 Antibodies

Nuclear factor of activated T-cells, cytoplasmic 4 (NFATC4)

Plays a role in the inducible expression of cytokine genes in T-cells, particularly in the induction of the IL-2 and IL- 4. Transcriptionally repressed by estrogen receptors; this inhibition is further improved by estrogen.

Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation.

Gene Information

Human
Gene Name:	NFATC4
Uniprot:	Q14934
Entrez:	4776

Family

Belongs to:
No superfamily

Alternative Names

NF-AT3; NFAT3nuclear factor of activated T-cells, cytoplasmic 4; NFATc4; NF-ATc4; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4; T cell transcription factor NFAT3; T-cell transcription factor NFAT3

Molecular Weight

Mass (kDA):
95.449 kDA

Genomic Context

Human
Location:	14q12
Sequence:	14; NC_000014.9 (24366911..24379604)

Tissue Specificity

Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.

Subcellular Localizations

Cytoplasm, cytosol. Nucleus. When hyperphosphorylated, localizes in the cytosol. When intracellular Ca(2+) levels increase, dephosphorylation by calcineurin/PPP3CA leads to translocation into the nucleus (PubMed:11997522, PubMed:18347059). MAPK7/ERK5 and MTOR regulate NFATC4 nuclear export through phosphorylation at Ser-168 and Ser-170 (PubMed:18347059).

Diseases

• Hypertrophy
• Cardiac Hypertrophy
• Obesity
• Malignant Neoplasms
• Nervousness
• Inflammation
• Stenosis
• Neoplasms
• Inflammatory Response
• Alzheimer's Disease
• Diabetes Mellitus
• Hyperalgesia
• Cardiotoxicity

• Malignant Neoplasm Of Lung
• Insulin Resistance
• Nerve Degeneration
• Insulin Sensitivity
• Hypertensive Disease
• Heart Failure
• Heart Diseases

Interacting Proteins

• UBC

Pathways

• Localization
• Cell Differentiation
• Immune Response
• Cell Proliferation
• Inflammatory Response
• Secretion
• Osteoclast Differentiation
• Cell Activation
• T Cell Activation
• Proteolysis
• Nuclear Export
• Nuclear Import
• Neuroprotection

• Cell Death
• Pathogenesis
• Heart Development
• Regulation Of Cell Differentiation
• Rna Interference
• Transport
• Muscle Cell Proliferation

PTMs

• Phosphorylation
• Dephosphorylation
• Cleavage

• Acetylation
• Ubiquitination
• Reduction

• Ribosylation

Research Areas

• Transcription Factors and Regulators
• Wnt Signaling Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NFATC4

Boster Bio - Best Uses Of The NFATC4 Marker

It is vital to employ the right equipment when you conduct experiments in your lab. A Boster Microplate Reader is capable of reading absorbances at 450nm. A Boster Pipette can be used to dispense 0.5 milliliters up to 1 ml of solution in aqueous form. Multichannel pipettes are the best choice for large amounts of samples. Sharing your results can result in product credits.

Boster Bio Anti-GAPDH Antibody Picoband

This boster bio antibody is labeled as A00227-1. It is able to react with Human, Mouse, and Rat. It comes with 5 mg BSA to prepare your sample. The blocking peptide can be purchased for up to one month if looking to do more detailed research. The Boster Bio Anti-GAPDH Antibody Picoband utilizes the NFATC4 marker.

Boster Bio DAB Chromogenic Substrate Kit (Blue)

Boster Bio DAB Chromogenic Substrates kit is an instrument that can be used to stain tissue sections and cells. It is used in immunohistochemistry, immunocytochemistry, and cell culture. The buffer is stable at -20°C for one year. Boster Bio DAB Chromogenic Staining Substrate Kit Blue (Blue) is a safe and easy-to-use staining product.

The extracts were separated using 12% SDS-PAGE. The extracts were then electrotransferred onto membranes of nitrocellulose to find out if there was rEgGrx1 within the samples. Membranes were blocked using 5 % (w/v), skim milk. The membranes were incubated using sheep serum, rabbit anti–rEg–Grx1 IgG antibody (1.200 dilution) or goat anti–rabbit IgG. The membranes were then incubated with either a chromogenic substrate, or the Boster Bio DAB ChromogenicSubstrate Kit (Blue).