NFATC2 Antibodies

Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2)

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.

Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.

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Gene Information

Human
Gene Name:	NFATC2
Uniprot:	Q13469
Entrez:	4773

Family

Belongs to:
No superfamily

Alternative Names

NFAT pre-existing subunit; NFAT transcription complex, preexisting component; NFAT1; NFAT1nuclear factor of activated T-cells, cytoplasmic 2; NFATC2; NF-ATc2; NFATP; NF-ATp; NFATPnuclear factor of activated T-cells, preexisting component; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2; preexisting nuclear factor of activated T-cells 2; T cell transcription factor NFAT1; T-cell transcription factor NFAT1

Molecular Weight

Mass (kDA):
100.146 kDA

Genomic Context

Human
Location:	20q13.2
Sequence:	20; NC_000020.11 (51386963..51562857, complement)

Tissue Specificity

Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid. Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid.

Subcellular Localizations

Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.

Diseases

• Inflammation
• Neoplasms
• Malignant Neoplasms
• Immunologic Deficiency Syndromes
• Hypertrophy
• Hiv Infections
• Autoimmune Reaction
• Hypertensive Disease
• Carcinoma
• Lymphoma
• Idiopathic Pulmonary Hypertension
• Neoplasm Metastasis

• Mammary Neoplasms
• Pulmonary Hypertension
• Cell Transformation, Neoplastic
• Graft-vs-host Disease
• Asthma
• Allergy
• Chimera Disorder

Interacting Proteins

• CREB1
• JUN
• LRRK2
• USP22

Pathways

• Immune Response
• Cell Activation
• Localization
• T Cell Activation
• Pathogenesis
• Cell Cycle
• Secretion
• Cell Differentiation
• Cell Proliferation
• Cell Growth
• Osteoclast Differentiation
• Lymphocyte Activation
• Nuclear Export

• Nuclear Import
• Rna Interference
• Cytokine Production
• Cell Death
• Myoblast Fusion
• Translation
• Angiogenesis

PTMs

• Phosphorylation
• Dephosphorylation
• Acetylation
• Cleavage
• Methylation
• Sumoylation

• Reduction
• Glycosylation
• Oxidation
• Ribosylation
• Cysteinylation
• Ubiquitination

Research Areas

• Adaptive Immunity
• Immunology
• Transcription Factors and Regulators
• Wnt Signaling Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NFATC2

Best Uses Of The NFATC2 Marker

If you're working with the NFATC2 Marker, you've probably already encountered the NFATc2IP and UBE2I/UBC9 antibodies. Continue reading to learn more. Both antibodies can be used to determine the amount of protein in samples. This marker can be utilized in a range of applications that include biomedical research. Boster offers product credits to scientists submitting their results. Scientists from all over the world can apply for this credit program.

NFATc2IP

Boster Bio produced this antibody and tested it on several platforms. The antibodies were tested on known positive and negative samples. They were also tested for specificity and affinity. Boster also rewards scientists who evaluate the product with product credits. Boster also rewards all scientists across the globe. Continue reading to learn more about Boster Bio's NFATC2 anti-body.

UBE2I/UBC9

The NFAT2 gene encodes a protein that regulates myoblast fusion. It also regulates the growth of osteoclast precursor cells, which allows them to differentiate into multinucleated osteoclast cells. NFATC2 was discovered to stimulate osteoclast differentiation in this study. This study highlights the role this gene plays in the process of determining osteoclast differentiation.

Induction of CPMs

NFATc1 is essential for the induction of CPMs using the Nfatc2 marker. Mice lacking NFATc2 have a dysregulated NFATc1 promoter and multiple cytokine gene mutations. This discovery highlights the complexity of these transcription complexes. Although the mechanism of the NFATc1's function isn't fully understood but it is believed that it will work in concert with the AP-1 transcription factor to regulate cytokine gene expression.

NFATc2 is essential for optimal induction of NFATc1 expression in T cells. Its transcriptional function is non-negotiable however in ex vivo. The AT-rich region in the NFATc2 gene is responsible for the CPM-inducing activity in T cells. However, the NFATc2 gene-expression requirement for IL-2 expression in mice is not yet understood.

Many Th2 cytokines are controlled by the NFATc2 gene. While NFATc1 is the primary positive transcription factor for Th2 cells, the underlying mechanism for a lack of Th2 lymphokine expression is unknown. In addition, the increased nuclear NFATc2 may affect CD3g gene expression and thus inhibit the expression of TCR/CD3 on cells' surface.

Researchers have used PE-induced VSMCs to show that it blocks the activation of calcineurin as well as nuclear translocation of the NFATc1. By blocking the calcineurin activity, CsA inhibits NFATc1-mediated cell growth. Further research is needed to determine if the VSMCs that are induced by CsA are controlled by the calcineurin/NFATc1 signaling pathway.

After being pretreated with PE for nine hours or twenty-four, Quiescent VSMCs were incubated with a solution containing 0.55 mg CsA. Nuclear extracts were made using the same process as previously described. Nuclear extracts were then incubated with anti-NFAT antibodies for a period of 20 min. These antibodies were obtained from AffinityBioreagents.

NFATc2 also controlled epigenetic upregulation of chemokine CXCL14 in dorsal horn neurons, which contributes to chronic pain caused by paclitaxel. In addition to regulating axoplasmic transportation, NFATc2 regulates the expression of a number of cytokines. Although the mechanism responsible for the NFATc2-induced CPMs remains elusive it is possible the NFATc2 proteins may be responsible for chronic pain following nerve injury.

Incubation with DyLight 594 combined affinity Goat Anti-Rabbit IgG

Incubation with DyLight 594, a goat anti-rabbit antibody was conducted using CPMs. After 48 hours the sections were fixed with paraformaldehyde at 4 and then washed three time with PBS and then infiltrated by 0.3% Triton X-100, or normal goat serum for 30 min at 37°C. Then, goat anti-rabbit IgG was added for 1 h at 37degC and images were taken under a fluorescence-inverted microscope.

The instrument is extremely specific and can be utilized in a variety test. It can be utilized in immunofluorescence microscopy, ELISA and Western blot. This antibody is also compatible with IP and FACS. It can be stored at -20degC. It reacts with rabbit heavy chain IgG proteins, but not non-immunoglobulin-se proteins because it is affinity-purified. It's also highly sensitive and has high specificity. It is not resold unless express authorization is obtained.

Biolayer interferometry is a different method to test affinity. To accomplish this it was necessary to add porcine APN to a high precision streptavidin biosensor. After that, they were submerged in PBS+Tween-20+1 percent BSA. Data were then fit using an 1:1 local fit. Once the results were obtained then they were compared with the results from ELISA and flow cytometry as the controls.

You can also include digestive enzyme inhibitors in your gut to increase the stability of your antibody. Incorporating antibodies can also enhance their stability. This prevents them from being broken down. In this instance the antigen-presenting cells soak up the antibody and process it.

The antibody targeted by FedF had greater immunogenicity than untargeted mIgG1. The antigen-specific IgA serum response was greater than the non-targeted APN antibody. These results cannot be attributed to a significant dose of antibody. Instead, the results suggest that the DyLight 594 combined affinity Goat Anti Rabbit IgG from Boster Bio antibody is extremely effective and efficient in treating a wide range of cancers.

Incubation with DyLight594 boosted the amount of a-APN-mIgG1 that was absorbed into the loops that are ligated. In a gut ligated loop experiment, the presence of DL755-labelled IMM013 and draining of MLN were demonstrated.