NELFB Antibodies

Negative elongation factor B (NELFB)

Essential part of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts through an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.

The NELF complex is included in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which might help to stabilize the NELF complex on nucleic acid.

Gene Information

Human
Gene Name:	NELFB
Uniprot:	Q8WX92
Entrez:	25920

Family

Belongs to:
NELF-B family

Alternative Names

Negative elongation factor B

Molecular Weight

Mass (kDA):
65.697 kDA

Genomic Context

Human
Location:	9q34.3
Sequence:	9; NC_000009.12 (137255327..137273542)

Tissue Specificity

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.

Subcellular Localizations

Nucleus.

Interacting Proteins

• BRCA1
• DENR
• FYN
• GRB2
• HSPA9

• KHDRBS3
• MGST1
• NCK1
• NELFE
• PIK3R1

PTMs

• Acetylation

• Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NELFB

Boster Bio - Best Uses Of The NELFB Marker

Boster provides high-affinity primary antibodies against the NELFB protein for Western blotting. Their antibodies have been validated by the research community for use in Western blotting, Immunohistochemistry, and ELISA. Boster's high-affinity antibody can provide the results you need. This antibody was tested in immunohistochemistry for NELFB protein detection in various tissue samples.

Anti-NELFB

Bostro Bio contains the Anti-NELFB Marker. This marker is an important component of the boster's immune system. This protein is crucial for the antitumor effect function of CD8+T cell. Boster Bio Anti NELFB Marker has been shown by CD8+ T cells to increase their tumor-killing capacity. Read on to find out more. This article will discuss the role of Nelfb within the Boster Bio Immunoassay as well as how it can be used for clinical trials.

The Anti-NELFB Marker in Bostro Bio is a highly specific antibody designed for studies of the protein. It recognizes the pol II pausing protein, which controls transcription. NELF has been implicated in several aspects in metazoan gene expression including energy metabolism in the heart, inflammatory response of macrophages, and myofiber healing after injury. The protein also maintains the integrity of the junctions.

A transgenic mouse model was used in a recent study to test the effects of NELFB overexpression. Tg mice exhibited NELFA/NELFC, which are vital for stabilizing NELF. These cells were then mixed with control T cells and transferred to B16 tumor-bearing recipient mice. After two or three weeks, CD8+ T cells were analyzed.

This study shows that CAR-T immunotherapy is more effective in mice when hNELFB is overexpressed. The hNELFB-overexpression-CAR-T cells showed a greater percentage of CD62L+CD45RA+ cells in the tumor-bearing mice, as compared to controls. Higher levels of memory-promoting and memory-promoting T cell were also observed in the hNELFBCAR28z T cells.

Other antibodies

The NELFB genes regulate the expression of genes involved fatty acid metabolism. The TILs of human melanoma are often enriched in Nelfbf/fCD8+ cell. These cells are often associated both with TCF7 deficiencies and aging. Boster Bio provides a variety NELFB-specific antibodies, reagents, and other tools to aid researchers in their analysis of the NELFB genes.

In several studies, the NELFB gene was used to study NELFA's role in tumor immunotherapy. NELFB overexpression can increase CD8+ T-cell activity. This marker is known to help differentiate the Nelff-producing cells from the rest of the population, which may play a role in the treatment of cancer. It can also be used to increase T cell memory and multifunctionality.

KO-mediated deletion of NELFb decreases chromatin accessibility by reducing Pol II pausing and TCF1-associated loci. The results suggest that Nelfb may be a key anti-tumor function. Nelfb has been shown to inhibit the accumulation Pol II, a transcription factor which increases tumor immunity. In addition, Nelfb ablation reduces chromatin accessibility.

Blotting in the West

The Western blotting with the NELFB markers can be used to detect the presence of proteins in cell culture media. These antibodies recognize the protein of interest and the intensity of the bands corresponds with the amount of the protein in a sample. This technique can also be used with other imaging systems.

This technique is crucial in the analysis of protein samples. Scientists can identify proteins using this technique. It can be used both in qualitative and quantitative applications. This technique involves the separation and transfer of proteins according to their size. The secondary and primary antibodies are then used to label them. Secondary antibodies can be fluorescently labeled in some cases to permit direct visualization of the protein. This technique can also be used in biochemistry, cell biology, and molecular biology.

The most common sample used for western blotting is cell lysates. It is important to ensure that the sample is as cold as possible to reduce the risk of denaturing the protein during the extraction process. In order to prevent protein destruction at low temperatures, it is a good idea add protease inhibitors. It is important not to forget that tissue samples can have a higher degree of structure than other materials and therefore need mechanical invention.

An enzymatic label is used for western blots with the NELFB marker. Alkaline phosphatase is the enzyme used. Its broad substrate range is what makes it superior to other enzymes. It has the disadvantages of a slower reaction time and a low signal-to noise ratio. It is not recommended that western blotting be used for quantitative analysis.

It is important that you remember to use two different types agarose gel when evaluating a protein using western blotting. Stacking gel is slightly acidic and contains lower levels of acrylamide. This allows for a more defined band to be formed. Basic separating gel contains a higher level of polyacrylamide. This narrows the pores of membranes and allows smaller proteins to move through the blot easier.

Applications

A transgenic mouse model was used to explore the functional implications the NELFB Marker has in relation to T cell specificity. NELFB transgenic mice expressing NELFA/NELFC probably stabilized NELF. To test NELFB-overexpression, T cell from these mice were combined with control T cell and then transferred to B16 cancer-bearing recipients. Tumor-infiltrating CD8+ T cells were measured two or three weeks after transfer.

The Carl H. June laboratory, University of Pennsylvania, generously donated the pELPS -CAR19 -28z vector. It includes the NELFB sequence for human genes as well as the P2A cleavage sequencing. VectorBuilder Inc., Gene Universal, Inc., are two companies that specialize in high titer viral vectors.