Nacc1 Antibodies

Nucleus accumbens-associated protein 1 (Nacc1)

Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells during recruitment of HDAC3 and HDAC4.

Contributes to tumor development, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1.

Gene Information

Mouse
Gene Name:	Nacc1
Uniprot:	Q7TSZ8
Entrez:	66830

Family

Belongs to:
No superfamily

Alternative Names

BEND8; BTB (POZ) domain containing 14B; BTB/POZ domain-containing protein 14B; BTBD14B; BTBD30; NAC1; NAC1BEN domain containing 8; NAC-1FLJ37383; NACC1; nucleus accumbens associated 1, BEN and BTB (POZ) domain containing; nucleus accumbens-associated protein 1; transcriptional repressor NAC1

Molecular Weight

Mass (kDA):
56.537 kDA

Genomic Context

Mouse
Location:	8 C2|8 41.02 cM
Sequence:	8;

Tissue Specificity

Ubiquitously expressed with higher expression in the brain, kidney and liver, and at lower levels in heart, lung and testes.

Diseases

• Malignant Neoplasms
• Carcinoma
• Neoplasms
• Ovarian Neoplasm
• Malignant Neoplasm Of Ovary
• Ovarian Serous Adenocarcinoma
• Neuroblastoma
• Cystadenocarcinoma, Serous
• Neoplasm Metastasis
• Neoplasm Invasiveness
• Epithelial Ovarian Cancer
• Adenocarcinoma

• Neoplasm Recurrence, Local
• Uterine Cervical Neoplasm
• Endometrial Polyp
• Cell Invasion
• Cell Transformation, Neoplastic
• Metastatic Malignant Neoplasm To The Lymph Node
• Malignant Tumor Of Cervix

Pathways

• Cell Proliferation
• Sensitization
• Localization
• Cell Growth
• Cell Migration
• Cell Cycle
• Oncogenesis
• Cytokinesis
• Interphase
• Drug Resistance
• S Phase
• Pathogenesis
• T Cell Selection

• Programmed Cell Death
• Cellular Senescence
• Proteolysis
• Regulation Of Gene Expression
• Regulation Of Autophagy
• Stem Cell Proliferation
• Response To Cocaine

PTMs

• Phosphorylation

• Deacetylation

• Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Nacc1

Boster Bio - Best Uses Of The NACC1 Marker

We will be discussing the Boster Bio Anti-Nac1/NACC1 agent and discussing the best uses of NACC1 markers. This article will also talk about the Boster Bi-Citrate buffer solution and Immunofluorescence. The following information should be read before you decide to purchase these products. If you have any questions, please don't hesitate to contact us.

Boster Bio Anti-Nac1/NACC1 Marker

Boster Bio Anti-Nac1/ NACC1Marker is a boster bio antibody that reacts with Human NAC-1, which is expressed in E. Coli. This product can be used in ELISA or WB. Boster Bio produces antibodies using high-affinity primaries antibodies. These antibodies have been extensively cited and validated for Western Blotting and Immunohistochemistry.

Boster Bio Citrate buffer treatment

This buffer solution is stable at room temperature for a year. It can be used to recover antigens that have been hidden by heat-induced epitope retrieval or cross-linking. Boster Bio Citrate buffer solution for the NACC1 marker is recommended for studies involving the analysis of cellular structures in human tissues. Boster Bio Citrate buffer for NACC1 marker can be used in conjunction with a wide variety of antibodies, even antibodies to the NACC1 gene.

IHC antigen retrieval reagent

When performing IHC experiments, optimal antigen retrieval is critical. There are two main methods: heat-induced epitope removal and protease–induced epitope retrieval. Heat-induced epitope retrieval generally has a higher success rate, but is not necessary for frozen cross sections. IHC experiments generally prefer neutral staining buffers. However, an alkaline staining option may not be available. In this case, an acidic solution, such as a sodium citrate buffer, might be used.

Boster Bio also develops ELISA kits and produces research antibodies for a variety applications. Its antibodies can detect biomarkers in neurosciences and developmental biology. These antibodies are available as monoclonal, polyclonal and mouse monoclonal versions. Those who purchase these products are entitled to a free secondary antibody.

Tissue samples were fixed in formalin, and non-tumorous tissues were embedded into paraffin to perform IHC. Slices were 4 um thick. Next, tissue sections were incubated for 30 minutes in 0.01M citrate buffer to disperse endogenous antioxidants. Next, nonspecific binding of the tissue was blocked with 10% goat sera. Anti-NAC1 antibodies (1:200 dilution), were applied to tissues and incubated at 30 min. Finally, tissue sections were incubated with anti-NAC1 antibody and peroxidase-labeled goat anti-rabbit secondary antibodies.

After antigen retrieval has been completed, tissue sections are prepared and cells are stained with Boster’s DAB Chromogenic Substrate Kit. This solution is colorless, clear, and has no precipitate. The sample preparation is done using a 4% Paraformaldehyde in PBS. Antigenic sites can then re-form after this step. Once these structures are formed, they are a good candidate for immunohistochemistry.

Immunofluorescence

Boster Bio Anti NACC1 Antibody is a reagent which reacts to Humans and Mouse. This antibody can be stored at -20degC for one week or at 4degC. Boster Bio products can only be used in laboratory and are not intended for diagnostic or in vivo use. Boster Bio provides more information about this marker on its website.

The NACC1 peptide size has an impact on its rheology and pharmacokinetics as well as stability and biological properties. NACs droplet sizes were determined before and during OVA addition. NAC1 did not interact with OVA. NAC2 & NAC3 had an increase in size when OVA was mixed with them. This interaction suggests NACs may facilitate antigen sampling by dendritic cells.

NAC3 stimulates the uptake of antigens in humans. When administered intranasally, it induces high levels of specific IgG antibodies, including IgG1 and IgG2. Interestingly, the NAC1 molecule also induces the production of a small number of IgG2c antibodies. The effectiveness of vaccines is dependent on the response to both isotypes.

As a candidate for the use in vaccines, NAC1-3 was validated by testing its cytotoxic and cell-tolerance levels in vitro. Three cell types were used for in vitro studies: macrophages, part of the immune systems, and airway epithelia.