MRGPRX1 Antibodies

Mas-related G-protein coupled receptor member X1 (MRGPRX1)

Orphan receptor. Probably involved in the function of nociceptive neurons.

Potently triggered by enkephalins such as BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22)(PubMed:26582731). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells.

Gene Information

Human
Gene Name:	MRGPRX1
Uniprot:	Q96LB2
Entrez:	259249

Family

Belongs to:
G-protein coupled receptor 1 family

Alternative Names

G protein-coupled receptor MRGX1; GPCR; MAS-related GPR, member X1; mas-related G-protein coupled receptor member X1; MRGX1; MRGX1G protein-coupled receptor SNSR3; Sensory neuron-specific G-protein coupled receptor 3/4; SNSR3; SNSR4

Molecular Weight

Mass (kDA):
36.25 kDA

Genomic Context

Human
Location:	11p15.1|11
Sequence:	11; NC_000011.10 (18933813..18935002, complement)

Tissue Specificity

Uniquely localized in a subset of small dorsal root and trigeminal sensory neurons.

Subcellular Localizations

Cell membrane; Multi-pass membrane protein.

Diseases

• Pruritus
• Disease Of Adrenal Medulla
• Hyperalgesia
• Welts
• Pain
• Dysesthesia

PTMs

• Cleavage

Research Areas

• GPCR

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MRGPRX1

Best Uses Of The MRGPRX1 Marker

MRGPRX1 is a gene that encodes the MAS-related G protein coupled receptor, member A. It is used for the detection of the protein in samples. This ELISA kit uses primary and secondary antibodies and has a Picogram sensitivity. This ELISA kit has been validated for a wide range samples and is highly sensitive. Images and validation procedure are available upon request. It uses Picoband, which is powered through insights from immunogen design. It is supported by Sanbio, a BeNeLux distributor.

Benefits of using MRGPRX1

MRGPRX1 is a gene that regulates the synthesis of the retinoic acid, a key component of the brain. When expressed in glial cells, MRGPRX1 is known to modulate the amount of retinol. To analyze the expression of this gene, the ISH test can be used. ISH was done using two specific mRNAs – MRGPRX1 & MRGPRD. The duplex assay (BaseScope), which was provided by Advanced Cell Diagnostics, Inc., was used to perform this test. The samples were stained with hematoxylin.

MRGPRX1 activation reduced spontaneous and evoked pain in mice significantly This is a major finding because MRGPRX1 has been found to be expressed in primary sensory cells. It modulates nociceptive transmission and activation may lead to pain-specific inhibiters. MRGPRX1 full agonists as well as PAMs could improve the selectivity in pain inhibition.

Two proteins are encoded by the MRGPRX1 protein gene. These proteins have different amino acid identities, but share approximately 54% of their amino acids. This may explain why agonists have been found in both the mouse and human MRGPRC11s, but not all of them have been tested for in vivo function. The MRGPRX1Marker is a rodent platform that allows for the screening of compounds against MRGPRX1.

MRGPRX1 may inhibit pain and function as an itch receptor at peripheral nerve terminals. BAM22 levels in chronic pain are higher in the spinal canal's superficial dorsalhorn. This area is also home to central terminals of nociceptive sensory neurons. The BAM22 level is below the limit of detection in skin. ML382 would optimize inhibition of spinal nerve transmission through endogenous BAM22.

MAS-related G protein coupled receptor member C is a human gene

One large family of orphan sensory receptors is the MAS-related GPCR (or GPCR), which is important for sensory functions. These receptors may play a variety of roles, including temperature and pain sensation, sleep, proliferation and desgranulation, and sleep. The neurons of the dorsal ganglia have the human gene Mas1.

A protein called "MRGPRX2" is encoded by MAS-related gene G-protein coupled receptor. This protein is found most abundantly in mast cells and other immune cell types. It has been shown that blocking the expression of Mas-related G-protein coupled receptors, such as MRGPRX2, leads to mast cell degranulation, or pseudo-allergic reactions.

The expression of MAS-related G protein-coupled receptor (MRGPR), is restricted to primary sensor neurons. It interacts with TRPA1's pain receptor. To study the signaling of MAS-related GPCR-X1 receptors in the brain, we used dental pulp. We have measured the membrane potential responses using FluoVoltTM and Fura-2-based Ca2+ imaging.

MRGPRX2 is a member the MRGPRXX family. It is also a novel GPCR to HK-1 in human MCs. Hemokinin-1, produced by activated mastcells, is also produced. This neuropeptide is involved in experimental chronic allergic respiratory inflammation. Asthma fatalities are also affected by an increased number of mast cells. Many mice have elevated levels of MRGPRX2 blood.

Investigate primary and second antibodies

There are many ways to detect the target protein using a primary antibodies. One way to detect a target protein is to immunize a host with an antibody of a different species. Anti-mouse secondary antibodies can be produced in goats vaccinated with mouse IgG. In addition to recognizing mouse IgG, this antibody binds to any mouse IgG fragment or class. An antibody that is specific for mouse IgG will be produced by a goat that has been immunized using mouse IgG1 antibodies.

Secondary antibodies may be conjugated with a variety labels. The downstream application depends on which type of label the secondary antibody is attached to. Secondary antibodies are usually conjugated to polyclonal antibody that is suitable for immunocytochemistry. If you want to detect antibodies that are associated with the MRGPRX1 indicator, you should research primary antibodies as well as secondary antibodies. This is the most efficient method of detecting the marker.

There are many methods to find primary antibodies using the MRGPRX1 marker. Primary antibodies can be made from a variety of host species, including rabbits, chickens, goats, and chickens. Secondary antibodies may be purchased ready-to use or may require further optimization. Liquid secondary antibodies can be stored at -20°C for up to a year. Thawed, these secondary antibodies are highly effective and can be used for multiple applications.

The primary antibody control confirms primary antibody binding to the antigen's epitope. It also demonstrates the specificity of binding to the antigen in the same conditions as the cell culture, fixation, and detergent treatment. Although many methods have been developed for proving the specificity or antigen specificity of primary antibodies, there is no universal method. We discuss four of these methods below.

Picogram sensitivity ELISA kit

Picogram sensitivity ELISA Kits for MRGPRX1 are designed to quantitatively determine low levels of human connecting peptides (hC-peptides) in serum or plasma. This product is for research use only. Human C-peptide has a half life of approximately three to 4 times insulin. It is useful for measuring beta-cell insulin secretion.

The ELISA kits can detect bilirubin. It is a known activator for murine and human MRGPRX1 genes. The assay is performed using HEK293 cells that have been incubated with FMRF or 50 mM bilirubin. The Ca 2+ imaging in the first panel (A) is a demonstration of the binding isotherms for bilirubin.

Boster Bio's Picokine ELISA system

Boster Bio develops high-sensitivity ELISA kits for detecting biomarkers in cancer, neurosciences, and development biology. These antibodies are sensitive to picograms. They can be ordered through tebubio. Boster Bio created an ELISA tool to measure MRGPRX1 mRNA.

This ELISA can be used with any type of detection method or antibody. However, it is recommended to use HRP detection chemistry. The type of assay, quality of the antibodies and optimization process determine the degree of improvement. Contact Sanbio at +1.784.212.212.com to learn more. Sanbio is also available for BeNeLux delivery and customized services.