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- Table of Contents
Facts about Mesoderm posterior protein 2.
Regulates also the FGF signaling pathway. Generates rostro-caudal polarity of somites by down-regulating in the presumptive rostral domain DLL1, a Notch ligand.
Mouse | |
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Gene Name: | Mesp2 |
Uniprot: | O08574 |
Entrez: | 17293 |
Belongs to: |
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No superfamily |
bHLHc6; class C basic helix-loop-helix protein 6; mesoderm posterior 2 homolog (mouse); mesoderm posterior protein 2; SCDO2
Mass (kDA):
39.789 kDA
Mouse | |
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Location: | 7 D2|7 45.18 cM |
Sequence: | 7; |
You've found the right place if you're searching for an antibody that detects Mesoderm posterior protein 2, (MESP2) Boster Bio Anti-MESP2 antibody is tested in WB applications. It reacts well with Rat, Mouse and Human samples. For best results in your experiments, use the Boster bio MESP2 marker antibody in WB applications.
The MESP2 marker recognizes the expression of Mesp2 in anterior presomitic mesoderm. Mesp2 expression contributes to the rostral -caudal pole. It participates in the formation of segment borders and regulates the FGF signaling pathway. It is also involved in cardiac mesoderm formation.
The MESP2 gene instructs a transcription factor to attach itself to specific DNA regions and control specific genes. The MESP2 protein also controls the genes of the Notch pathway, which plays a vital role in vertebrate development. MESP2 also regulates the segmentation of somites. This antibody is highly specific for mesoderm posterior protein 2, (MeP2), in humans, mice, and rats.
The next step in antibody discovery is to develop high-affinity primary antibodies by using the MESP2-marker. An error in affinity maturation causes persistent autoimmune disorder, and insufficient production IgM antibodies. This discovery has important implications on drug discovery. Continue reading to learn about this new technology as well as its implications for antibody research. We have previously described affinity maturation.
Affinity maturation of a monoclonal antibody requires binding affinity to a specific peptide. We measured the insulin-reactive IgM's affinity for different days to confirm affinity maturation. WT mice have high affinity insulin-IgM levels by day 52. IgD-deficient mice don't reach this level until day 72. Both types of mice developed diabetes, and both showed signs of the disease.
The MESP2-marker helped to develop high-affinity antibodies that were specific for antigen-valency. This allows for distinct IgM as well as IgG responses to the antigen. This technology is especially useful in the development of personalized immunotherapies for specific diseases. This technology is used in many different ways.
IgD is critical for controlling the durations of primary autoreactive IgM immune responses. Defective IgD blocks the production of protective, high-affinity IgM antibodies. The dysfunction of IgD leads to autoimmune diseases. The antibodies produced by these cells recognize both DNA and nuclear structures. During embryogenesis, mice with defective IgM have high levels of antidsDNA-IgG antibodies.
It has been shown that NP-KLH conjugates exhibit high affinity for target antigens. Monovalent antigen-binding antibodies have been shown to have lower IgG-reactive IgD/IgD antibodies. This is an important finding. However the MESP2-binding MESP2-binding-NP-KLH conjugates does not alter the IgD/M immune response.
Previously, researchers failed to isolate a particular mRNA from a target cell. These efforts have paved the way for this method of antibody discovery. These antibodies can also be used for research purposes. There are many publications on the subject and several books. For example, NusseR and Kaska's recent study has shown that a single gene in a mRNA can alter specific proteins' signaling properties.
To determine the specificity of these high affinity primary antibodies, we first determined how many MESP2-binding monoclonal IgG-UNLB immunoglobulins from rats. These antibodies could then be detected using biotin conjugated peptides. This technique is more accurate in detecting specific mAbs.
PMID: 9242490 by Saga Y., et al. Mesp2: a novel mouse gene expressed in the presegmented mesoderm and essential for segmentation initiation.
PMID: 10330372 by del Barco Barrantes I., et al. Interaction between Notch signalling and Lunatic fringe during somite boundary formation in the mouse.