MCUR1 Antibodies

Mitochondrial calcium uniporter regulator 1 (MCUR1)

Key regulator of mitochondrial calcium uniporter (MCU) required for calcium entry into mitochondrion (PubMed:23178883, PubMed:26445506, PubMed:27184846, PubMed:26976564). Plays a direct role in uniporter-mediated calcium uptake via a direct interaction with MCU (PubMed:23178883).

Probably involved in the assembly of the membrane components of the uniporter complex (uniplex) (PubMed:27184846). .

Gene Information

Human
Gene Name:	MCUR1
Uniprot:	Q96AQ8
Entrez:	63933

Family

Belongs to:
CCDC90 family

Alternative Names

Mitochondrial calcium uniporter regulator 1

Molecular Weight

Mass (kDA):
39.694 kDA

Genomic Context

Human
Location:	6p23
Sequence:	6; NC_000006.12 (13786549..13814994, complement)

Tissue Specificity

Ubiquitously expressed.

Subcellular Localizations

Mitochondrion inner membrane; Multi-pass membrane protein.

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MCUR1

Boster Bio - Best Uses Of The MCUR1 Marker

Boster Bio is a good choice if you are looking for primary antibodies with high affinity. Boster Bio's antibodies are highly cited and validated by the research community. Boster antibodies have been validated on Western Blotting, Immunohistochemistry, and ELISA. Here's how to use these antibodies to enhance your research. Continue reading to learn more. This article includes information about Boster Bio's MCUR1 marker.

Boster offers high-affinity prim antibodies

Boster Bio products may be a good option if you're looking for an antibody that will target this marker. They are well validated and have won many awards from the research community over the past 25 years. Their primary antibodies have been validated for ELISA, Western Blotting, and Immunohistochemistry. Boster is the largest supplier of antibodies for MCUR1 markers.

Primary antibodies are immunoglobulins made from the immune systems of the host animals. The building blocks of primary antibodies are called complementarity-determining regions. The subject of complementarity-determining regions is complex, and we recommend that you read about it in Wikipedia. Scientists make primary polyclonal antibodies by immunizing hosts with an antigen. They then measure and purify the antigen.

High-affinity primaries antibodies are highly specific. They have been validated against a panel that includes 250 tissues. They also undergo extensive validation against untransfected cell lines. A reagent with a demonstrated specificity is important, especially if it cannot be used on multiple samples. Using a negative control will help determine if the antibody reacts with nonspecific proteins and prevent false-positives.

This primary-secondary antibody system allows for dual-labeling specimens. Dual-labeling makes it possible to ask more questions of the specimen. This allows for better answers and contextual data. Boster Bio's antibodies are a versatile, efficient choice for your research. The MCUR1 marker works in many tissues.

MCUR1 Marker

Using MCUR1 marker in a mouse cell line is easy to study phenotype variations. In a mouse-cell model, MCUR1 knockdown led to an increase in epithelial markers expression and a decrease of mesenchymal ones. Alternatively, MCUR1 expression had the opposite effect. Western blot analysis confirmed all results of the IF experiment. Moreover, MCUR1 caused an increase in the expression of Snail (an EMT-related transcription factor), while Slug was unaffected.

HCC cells had MCUR1-overexpression which promoted intrahepatic inflammation and distal pulmonary mesastases. These results support the hypothesis that MCUR1 promotes EMT. Similarly, overexpression promotes lung metastasis. The knockdown of MCUR1 stops lung metastasis and reverses liver metastasis. The MCUR1 gene is highly unstable. It is important not to forget that the expression levels of this gene are highly variable in different types of cells. Therefore, it can be difficult to determine what role it plays in each patient.

MCUR1 can also be used to monitor mesenchymal transformation. It is involved both in migration and invasion. Overexpression of MCUR1 promotes mesenchymal transition. This marker is therefore useful for many applications. Its role in cell migration and invasion remains unknown. It is worth noting however that it has been used in mesenchymal transition, cell movement, and cell invasion studies.

MCUR1

This molecular target promotes EMT because it binds to the MCUR1 Protein. Overexpression of MCUR1 promoted lung metastasis and intrahepatic migration. Knockdown MCUR1 reduced invasion and migration, and reversed inhibition of lung metastasis. This molecular target has the potential to help prevent metastasis in a number of human diseases.

This molecular targets was designed to assist scientists in a variety if research settings. GenePharma synthesized a small hairpin RNA(siRNA), targeting human MCUR1, from its cDNA. The siRNA primers have been determined using a standard, PCR reaction. The PV-Mito construct was established using the pAc1GFP1-Mito vector and adenovirus system.

Human cancers often have a high level of MCUR1 transcription. This could be due to MCU-1's anti-inflammatory and inflammatory effects. Although MCUR1 has been proven to support cancer cell growth, its role as a metastasis agent is still unknown. However, it is thought to be involved in tumorigenesis. Although it is still unclear what role MCUR1 has in cancer progression and development, its upregulation can be a strong indicator about the protein's tumorigenicity.