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Facts about Mast/stem cell growth factor receptor Kit.
Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase.
Human | |
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Gene Name: | KIT |
Uniprot: | P10721 |
Entrez: | 3815 |
Belongs to: |
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protein kinase superfamily |
CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
Mass (kDA):
109.865 kDA
Human | |
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Location: | 4q12 |
Sequence: | 4; NC_000004.12 (54657928..54740715) |
Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
The DAB Chromogenic Substrate Kit Blue is a multi-purpose marker that can be used to detect KIT antibodies. It is used to identify specific molecules in tissues and cells. Its high specificity and affinity make it to be used for many purposes. Boster Bio rewards its first reviewers with product credits. Scientists across the globe can avail the DAB Chromogenic Substrate Kit from Boster.
Boster Bio offers an Anti PAX8 Monoclonal Immunoglobulin. It reacts with Rat, Mouse, and Human. This antibody has been tested for the sensitivity of ICC, IF and IHC applications. The antibodies are manufactured in Trehalose and include a blocking peptide, which can be purchased separately. This antibody has a MW of approximately 60 kDa. It can be stored at 4°C for one month.
The company manufactures high-quality monoclonal antibodies for various applications. These antibodies are manufactured in-house using the most frequently used clones. They are also available in several conjugates. Customers can choose the one they want at the checkout.
Polyclonal antibodies are also available from many other companies. Abnova Biotechnology is a USDA and NIH accredited facility that produces monoclonal and polyclonal antibodies. Their specialization is developing custom antibodies, and they also provide peptide conjugation and ELISA services. Noble Life Sciences is a company that specializes in monoclonal antibody manufacturing and production of peptide libraries.
A peptide chain comprising 1160-1493 amino acids serves as an antigen in this antibody. Ki67 is found in the nucleus of human colon SKOV-3 cells and the SV40 transformed African green monkey (Cercopithecus aethiops).
The RAF1 is a serine/threonine-protein kinase that functions as a regulatory link between Ras GTPases. It plays a significant function in cell fate decisions. It activates a series of mitogen-activated proteins kinases activating dual specific MAPK Kinases, as well as extracellular signal-regulated Kinases. It also inhibits the differentiation erythroid cells.
In this study, total RNA was extracted from granulosa cell lines and the ovarian tissues using the RNAprep purified Micro Kit. The RNA was reverse-transcribed with the PrimeScript RT reagent (Takara). Final, the amplification was achieved by real-time PCR using sequence detection systems and a SYBR Ex TaqII premix agent.
Monoclonal antibody, UMAB107, can detect Ki-67 in breast tissue. It can also detect the protein in healthy people's colons. Its unique structure makes it useful in many diagnostic procedures. This invention also explains its use in immunohistochemistry. Monoclonal antibodies exhibit high binding specificityand sensitivity and can be used to diagnose.
After washing the cells, the membranes were are incubated using monoclonal antibodies of mouse and rabbit that specifically target RBP4, the HMGA1, and SF-1. After centrifugation, supernat and HAT culture medium were added. The cells were then placed in a moist box. The temperature constant in the incubator was 37 DEG Celsius and 5% CO2.
Anti-PROM1 (PROM1) antibodies are available from a number of sources. These antibodies recognize PROM1, an antigen that can be found both on the Human- and Mouse genomes. The antibodies have been tested using known positive and negative samples. Several platforms are used for validation and verification of antibodies. Read on to learn more about Boster Bio's anti-PROM1 antibody.
This monoclonal anti-PROM1 antibody identifies one polyclonal chain inside the PROM1 gene. This monoclonal antibody also compatible with various ELISA platforms. It also allows users to easily transfer the protein from one gel to the next. We recommend using at minimum three or two antibodies at a time for best results. It is possible to keep the antibodies in a freezer or refrigerated condition.
Boster Bio produces ELISA kits for research, in addition to polyclonal antibodies. These kits detect biomarkers related to neuroscience, cancer biology and research. There are more than 12,000 available antibodies from the company. The company's products have been scientifically validated against cells that were not transfected and recombinant lines of protein. In addition to this the majority of Boster Bio products are supported by technical support.
This DAB Chromogenic Substratum Kit contains horseradish peroxidase that can detect specific antibodies. DAB reacts with peroxidase to create an insoluble blue precipitate. DAB is applied to tissues and cells to observe the localization of HRP-conjugated antibodies. Boster Bio's DAB can be carcinogenic. It should be disposed of and used in good laboratory practices.
PMID: 2448137 by Yarden Y., et al. Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand.
PMID: 1279499 by Giebel L.B., et al. Organization and nucleotide sequence of the human KIT (mast/stem cell growth factor receptor) proto-oncogene.
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