Kallikrein-13 Antibodies

Kallikrein-13 (KLK13)

Gene Information

Human
Gene Name:	KLK13
Uniprot:	Q9UKR3
Entrez:	26085

Family

Belongs to:
peptidase S1 family

Alternative Names

DKFZp586J1923; EC 3.4.21; EC 3.4.21.-; Kallikrein 13; Kallikrein-like protein 4; kallikrein-related peptidase 13; KLK13; KLK-L4kallikrein-13; KLKL4kallikrein-like gene 4

Molecular Weight

Mass (kDA):
30.57 kDA

Genomic Context

Human
Location:	19q13.41
Sequence:	19; NC_000019.10 (51055626..51065106, complement)

Tissue Specificity

Expressed in prostate, breast, testis and salivary gland.

Subcellular Localizations

Secreted.

Diseases

• Malignant Neoplasms
• Neoplasms
• Malignant Neoplasm Of Ovary
• Malignant Neoplasm Of Breast
• Carcinoma
• Ovarian Neoplasm
• Lung Neoplasms
• Adenocarcinoma
• Malignant Neoplasm Of Lung
• Neoplasm Recurrence, Local
• Non-small Cell Lung Carcinoma
• Salivary Gland Diseases

• Malignant Paraganglionic Neoplasm
• Dermatologic Disorders
• Mammary Neoplasms
• Psoriasis
• Dermatitis, Atopic
• Nervousness
• Residual Tumor (qualifier Value)

Pathways

• Pathogenesis
• Localization
• Cell Migration
• Proteolysis
• Immune Response
• Complement Activation
• Autolysis
• Humoral Immune Response
• Demethylation
• Chemotaxis

• Reverse Transcription
• Cell Motility
• Tissue Remodeling
• Angiogenesis

PTMs

• Cleavage

• Demethylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/KLK13

Best Uses Of The KLK13 Marker

If you're a scientist who works with antibodies, you've probably wondered what the best way is to optimize your experiments. Here are some tips for optimizing your experiments. You'll need to choose the best antibody concentration. It is also necessary to make sure that the blocking buffer is well concentrated. Boster Bio's optimization guidelines will help you make your experiments more efficient. This way, you can optimize your ELISA experiments with minimal effort.

Steven Boster

Steve Boster, born June 6, 1922, passed away in Madison, WI, on Sunday, June 26, 2022, at age 63. He was a retired sales manager and a U.S. Army Veteran. He was also a Concordia Hall member from Staunton, VA.

Steve loved southern Gospel music, which he performed in his own home and sang so low in the public. He loved sports, especially his favorite team. He was also a huge fan of auto racing. He never missed a Friday night at the local racetrack. He attended additional events on weekends, including dirt track race. Despite his busy schedule he was always at the track. Here are his favorite uses of KLK13 Marker

Primary antibodies with high affinity

The KLK13 gene marker was used to determine the genetic determinant of affinity-matured antibodies. It was a promising approach to studying the immune reaction to a target antibody. This approach has many benefits over traditional methods, including lower costs, greater reproducibility and higher productivity. It allows for the reconstruction and analysis of antibody clonal lines. Next-generation sequencing provides high-quality data for inferring the amino acid sequences of germ line progenitor cells, which may differ from their true, unmutated ancestry. It allows for high-confidence identification of mutations within the VL and VH gene segments, but it is not able to determine the original sequence of VLJLJH. The original sequence of the VLJLJH junction is unreliable, and insertions and deletions during affinity maturation cannot

The CH58 antifluenza bNAb antibody has a strong affinity against the V2 protein of the HIV-1 HIV-1 envelope. It is located close to V2 Lys171 within the virus envelope glyprotein. The VL-Asp sequence is present near the V2 Lys171 residue and stabilizes the salt bridge between it and the HIV-1 Env.

This antibody can withstand temperatures exceeding 60°C, in addition to its affinity against antigens. However, despite its high affinity, antibodies can be difficult to separate from antigens when their concentration is high enough, which may lead to more complexities in the elution process. The high affinity of the antibody requires the use of harsh methods, such as elution from an affinity column.

Understanding the evolution in these repertoires is not only possible through affinity maturation. It is also important to understand other mechanisms that influence the ability of antibodies recognize target antigens. These methods, which include next-generation sequencing, allow reconstruction of antibody clonal linesages that have evolved in response viral pathogens. These studies have provided important insights into how affinity maturation works. The KLK13 genetic marker is a good choice for affinity-matured antibodies.

Both antibody types share similar binding site structures. Both antibodies have VHCDR3 loops with long lengths that pack against Asn332 glycon. These loops penetrate the glycan barrier to reach the Env protein surface. While the PGT124 and PGT122 are identical, the insertion in the KLK13 gene allows them to avoid neighboring glycans and achieve productive interactions with Asn137.

KLK13 marker

This paper presents a series of studies that show the activity of KLK13 in protein-protein interaction markers. In these studies, we used buffers ranging from pH 3.5 to 10.0 in 0.5-molar increments, a Wallac Envision fluorometer set at 355nm for excitation and 460nm for emission. The activity of KLK13 could be calculated by subtracting fluorescence produced by enzyme-free reactions and the fluorescence generated from the KLK13 mark. All reactions were performed at 37 degrees Celsius in triplicates and on 2 separate working days.

After identifying and inserting the gene of interest into a vector pPIC9, we then inserted it. The supernatant then was purified using ultraconcentration as well as cation exchanging chromatography. The cells were then treated with BMMY media to express the recombinant proteins. The mass spectrometry was used for analysis of the purified proteins. It was found to have a 450-nm protein weight, which confirmed its identity.

A bispecific antibody is useful for localizing cytotoxic drugs to KLK13 expressing cells. Bispecific antibodies consist of an arm which binds KLK-13. Another arm binds a different polypeptide. This anti-KLK13 antibody arm can be combined with a triggering molecular on a leukocyte such as the CD64/CD32fusion protein. After the anti-KLK13 antibody arms has been prepared, it should not be stored below 4°C or 20°C. It should be stored with a carrier protein, such as 0.1% HSA and BSA. Ensure that the KLK13 antibody solution has a concentration greater than 90% of KLK13 protein.

The KLK13 gene expression level is quantified by real-time PCR. To do so, total RNA prepared from breast tissues was serially diluted and the fluorescence signals were plotted against log of copy number. The standard curve can be derived by plotting cross points of the fluorescence signals against the log number. In this way, KLK13's signal is proportional to tumor size and grade.

KLK13 is a member of the kallikrein family of serine proteases. It is found in many human tissues, including the breast, testis, and prostate. KLKs are regulated by steroid hormones. KLK13 expression was evaluated in 173 epithelial and breast cancer patients. The optimal cutoff point was equal to 40th percentile, and the results showed that KLK13 expression significantly increased disease-free survival.

Optimization tips

The boster bio optimizer is a valuable tool for ELISA research. Its gene infographics give basic information about each gene and cover all genes in both mouse and human. Boster has a search bar that lets you look for genes of interest. This is especially useful if you are looking for the effect of a particular gene on the human genome. This is a great way to learn about the marker and how to optimize your experiment.