Protein jagged-1 Antibodies

Protein jagged-1 (JAG1)

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling (PubMed:18660822, PubMed:20437614). May be involved in cell-fate decisions during hematopoiesis (PubMed:9462510).

Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity).

Gene Information

Human
Gene Name:	JAG1
Uniprot:	P78504
Entrez:	182

Family

Belongs to:
No superfamily

Alternative Names

AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

Molecular Weight

Mass (kDA):
133.799 kDA

Genomic Context

Human
Location:	20p12.2
Sequence:	20; NC_000020.11 (10637684..10673999, complement)

Tissue Specificity

Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.

Subcellular Localizations

Membrane; Single-pass type I membrane protein.

Diseases

• Alagille Syndrome
• Malignant Neoplasms
• Neoplasms
• Haploinsufficiency
• Malignant Neoplasm Of Breast
• Congenital Heart Defects
• Tetralogy Of Fallot
• Carcinoma
• Cholestasis
• Stenosis
• Liver Diseases
• Mammary Neoplasms
• Cell Transformation, Neoplastic

• Developmental Disabilities
• Congenital Abnormality
• Pulmonary Stenosis
• Heart Diseases
• Tumor Angiogenesis
• Colorectal Cancer
• Tissue Adhesions

Interacting Proteins

• CD46
• NOTCH1

Pathways

• Notch Signaling Pathway
• Cell Differentiation
• Cell Proliferation
• Pathogenesis
• Cell Cycle
• Angiogenesis
• Neurogenesis
• Regeneration
• Lateral Inhibition
• Cell Fate Determination
• Ear Development
• Cell Fate Specification
• Localization

• Methylation
• Dna Methylation
• Liver Development
• Bone Development
• Cell Adhesion
• Inner Ear Development
• Cell Death

PTMs

• Cleavage
• Methylation

• Phosphorylation
• Glycosylation

• Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/JAG1

Boster Bio - Best Uses Of The JAG1 Marker

This article will cover the Jagged-1 proteins and their functions. We will also be discussing its signaling, and cellular interactions. Continue reading to learn more. Listed below are the best uses of the JAG1 marker. We hope this information was helpful to you. If not, feel free to leave a comment down below. Enjoy reading this article. Don't forget to share this article with your colleagues.

Jagged-1 protein

Boster Bio's newest drug, a peptide called Jagged-1, combines the strength of Jagged-1 and a proven mechanism to action. Jagged-1 consists of a protein with a Notch-like ligand. It is involved with the development of human carcinoma and is associated to poor prognosis. It has been shown in studies to activate Notch signaling. However, the upstream control of Notch activation remains unclear.

Jagged-1 is able to influence cell behavior, growth, and behavior in addition to its ability to target Notch receptors. Jagged1 protein level drops when it is expressed in lung cancer cells. The molecule's ability regulate Notch signaling is an important mechanism in regulating a wide range of developmental processes. Notch signaling is responsible for cell proliferation and pattern formation. Jagged-Notch signaling also regulates cell differentiation.

It has been shown that many types of cancer are caused by the JAG1 mutation. Affected individuals with the disease have one copy of the gene with defects in function. Jagged-1 also appears to regulate gene activity. In addition, the protein has a strong genetic influence on cancer risk and treatment outcomes. It is therefore crucial to develop treatments that effectively block the disease.

High levels of Jagged-1 and NOTCh2 co-expression has been linked to poor survival rates in breast cancer patients. These proteins have been implicated in many processes, including cell migration. Notch2 ligands are processed by the alpha-secretase, presenilin/gamma-secretase, and gamma-secretase. Notch receptors then release signaling fragments.

Function

Although JAG1 is an important tumor biomarker for many reasons, its exact function is not well understood. It has been established that tumors expressing higher amounts of JAG1 are more like luminal-like types of cancer. Functional studies in mice with breast cancer have also confirmed its significance. Here, we briefly describe its functions as well as cancer-related functions. We also discuss the potential therapeutic applications of JAG1 to treat a variety types of cancers.

Alagille syndrome is caused by mutations in the JAG1 gene. It affects one in every 70,000. This syndrome is autosomal dominant and causes developmental abnormalities in the skeleton, liver, kidney and heart. JAG1 mutations can result in cholestatic and biliary disease in a subset. Although the precise function of JAG1 is still unclear, some studies have shown that it has a role in the development of cancer and other inflammatory diseases.

Around one-third (or more) of Hensen cells is lost in the cochlears of Jag1 mutant mice. The mechanism for upregulation of CD44 and downregulation of SOX2 in these mutant cochlear cells was found to be similar to JAG1-Notch signaling in vestibular prosensory cell maintenance. JAG1 prevents nonsensory progenitors becoming prosensory by preserving SOX2 and antagonizing transcription factor LMX1A.

JAG1 is implicated in Notch signaling regulation and tumor growth. JAG1 promotes cell death and survival in tumor cells. It also influences development of tumor microenvironment, which includes the vasculature. JAG1 is a promising therapeutic candidate. It plays an important role for the development of many types cancers.

Signaling

There are many public databases that can assist with your research. One such database is Genbank. This site provides information about the genetic sequences for Delta 1, Delta like 1, Delta-like 3 and Delta-like 3. You can also make your own antibodies using a commercially-available siRNA. Below are some examples which show the JAG1 markers' best uses. They are listed alphabetically.

Jagged1 interacts with the JAK/STAT3 pathway. In addition, it promotes aberrant EMT and is a key player in the migration and invasion of platinum-resistant ovarian cancer. These results are not conclusive, however. Boster Bio: Best Uses for the JAG1 marker

Molecular interactions

The JAG1 protein is essential for determining the fate of cells. However, this approach still has limitations. As I have said, the data contained in the manuscript are not sufficient to draw conclusions. To support the conclusions presented in the manuscript, a number of experimental experiments are necessary. The paper must contain quantitative analyses. The soluble vectors form tumors faster than the control and empty vectors. They do not form tumors larger than 1500 mm3 and should therefore be assessed further to determine their potential.

The results of this study suggest that Jag1 negatively regulates Dll4mediated Notch activation. As a result, cells display a reduced endothelial function and promote haematopoietic speciation. To prevent haematopoietic specification, it is essential to block Dll4.

The AGM regions were isolated from E11.5 mouse embryos. The cells were fixed in 4% paraformaldehyde and left to dry for 40 minutes. After that, cells were placed on OP9-Jag1 slide and incubated at 37°C. After five days, the colonies could be counted. The FC values were calculated by comparing the values with the median value of the genes within each tissue.

During implantation, the OP9 stromal cell line was overexpressed with Jag1 and its ligands. These cell lines were cultured in a-minimum essential medium (AEM), which contained 20% fetal bovine serum, 1% penicillin/streptomycin, and 10% FCS. These cells were plated at 1.5 x 104 cells cm-2 24 hours before the experiment.

Cell fate specification

Boster bio's study found that Jag1 in embryos causes a decrease in the expression of her6 (and her9) in vivo. This study uses clones of the emilin3a promotor element to reveal the precise functions of Jag1.

Jagged1 regulates multiple cell fate decision making. Jagged1 regulates differentiation in the lung. It also controls Notch signaling. Jagged1 has been identified in the literature as a potential therapeutic target. Its ligands could be used for a variety of purposes, including cancer therapy and diagnosis.