Isl2 Antibodies

Insulin gene enhancer protein ISL-2 (Isl2)

Transcriptional factor that defines subclasses of motoneurons that divides into columns in the spinal cord and pick different axon pathways. .

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Gene Information

Rat
Gene Name:	Isl2
Uniprot:	P50480
Entrez:	57233

Family

Belongs to:
No superfamily

Alternative Names

FLJ10160; insulin gene enhancer protein ISL-2; ISL LIM homeobox 2; ISL2 transcription factor, LIM/homeodomain, (islet-2); ISL2; Islet2; Islet-2

Molecular Weight

Mass (kDA):
39.675 kDA

Genomic Context

Rat
Location:	8q24
Sequence:	8;

Diseases

• Nervousness
• Pneumonia
• Reperfusion Injury
• Ischemia
• Spinal Cord Injuries
• Demyelination
• Diabetes Mellitus
• Crest Syndrome
• Diabetes Mellitus, Non-insulin-dependent
• Cataract
• Congenital Heart Defects

• Hypoplasia
• Pituitary Diseases
• Inflammation
• Truncus Arteriosus, Persistent
• Chimera Disorder
• Cold Ischemia

Pathways

• Neurogenesis
• Regeneration
• Cell Death
• Neural Tube Closure
• Transposition
• Cell Proliferation
• Programmed Cell Death
• Neuron Development
• Inner Ear Development
• Autolysis
• Localization
• Myelination
• Tube Closure

• Heart Formation
• Translation
• Tissue Regeneration
• Ear Development
• Generation Of Neurons
• Cell Differentiation
• Innervation

PTMs

• Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Isl2

Boster Bio - Best Uses Of The ISL2 Marker

The ISL2 mark has become an integral part cell biology research. Scientists have many benefits from its use. Its flexibility allows scientists submit results for species-specific studies or applications. Boster scientists can even receive product credits for their work. This program is available to scientists from all backgrounds and at all levels. Learn more about the program's benefits and uses. This article will focus primarily on the Boster Bio Anti-ISL2/Islet2 and the Boster Bio Cell-Based ELISA Kit.

Boster Bio Anti-ISL2/Islet 2 Antibody

You've come to the right spot if you're looking for an ISL2 antibodies. Boster Bio is a top manufacturer of antibody kits that can be used in many types of molecular biology applications. The company has also recently expanded into providing products for PCR-related molecular biology applications. Boster Bio offers ELISA kits and other services that can be used by scientists to create custom assays. Boster Bio offers 24 hour technical support and free resources to researchers.

Human Islet-1 can also be detected in sections of the pancreas that have been embedded in paraffin-embedded sections. Islet-1 staining indicates that Islet-1 is localized in the nuclei islet cells. The Islet-1 antibody was diluted at 10 ug/mL for three hours at room temperature. It was counterstained using DAPI to confirm its presence.

Thyroid medullary carcinomas and paragangliomas/pheochromocytomas are two examples of extrapancreatic neuroendocrine neoplasms. These tumors do not have the ISL2 marker. Melanomas, on the other hand, have no role in ISL1-expressing tumor cells' proliferation or invasion.

ISL1 belongs to the LIM-homeodomain transcription factors subfamily. It is expressed mostly in pancreatic islet cells during adulthood and in the central nervous system. ISL1 is involved in pancreatic embryogenesis. Hence, it could be a useful marker for pancreatic islet cells. However, it appears that the protein plays important roles in postnatal pancreatic hormone production.

ISL1 was previously studied using human melanoma cells A375. To study proliferation, invasion, death and apoptosis in A375/ISL cells, a lentiviral virus containing ISL1 homeodomains was used. Gibco Life Technologies medium was used for the culture of A375/ISL cell lines. The proliferation of A375/ISL cell lines was then assessed using flow cytometry and MTT assays. Cell invasion was evaluated using Transwell assays.

Boster Bio Cell-Based ELISA kit

Boster Bio Cell - Based ELISA test for the ISL2 marker is a reliable, simple method to determine if your samples are containing the ISL2 proteins. This kit contains 96-well clear-bottom microplates for cell culture, 10x TBS and a 15x wash buffer. It also contains a 100x Rabbit polyclonal Anti–eIF2alpha/EIF2S1 antibody as well as a mouse monoclonal Anti–GAPDH antibody.