DNA helicase B Antibodies

AND HELB ELISA kits, DNA helicase B Proteins

Many biological assays use antibodies to detect DNA helicase B, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DNA helicase B in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DNA helicase B antibodies...

We validate the specificity of these antibodies to DNA helicase B by testing them on tissues known to express HELB positively and negatively. Browse below to find the HELB antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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HELB Infographic by Boster Bio

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HELB (NM_033647) Human Recombinant Protein

$1249
Cat # PROTQ8NG08
20 µg

HELB (NM_033647) Human Over-expression Lysate

Human

$960
Cat # LS092797
100 µg

HELB Overview

Facts about DNA helicase B.

HELB 3D structure. Source: alphafold

DNA helicase B (HELB)

Recruitment to single-stranded DNA (ssDNA) following DNA damage contributes to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB (PubMed:26774285). As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation (PubMed:26774285).

Acts independently of TP53BP1 (PubMed:26774285). Unwinds duplex DNA with 5'-3' polarity.

Gene Information

Human
Gene Name:	HELB
Uniprot:	Q8NG08
Entrez:	92797

Family

Belongs to:

RecD family

Alternative Names

DNA helicase B; hDHB; helicase (DNA) B

Molecular Weight

Mass (kDA):
123.252 kDA

Genomic Context


Human

Location:	12q14.3\|12q
Sequence:	12; NC_000012.12 (66302493..66343643)

Tissue Specificity

Highly expressed in testis and thymus and weakly in liver, spleen, kidney and brain.

Subcellular Localizations

Nucleus. Cytoplasm. Chromosome. Predominantly nuclear (PubMed:15146062). Phosphorylation at Ser-967 by CDK2 during the G1/S transition results in its nuclear export into the cytoplasm as cells approach and progress through S phase (PubMed:15146062). Following DNA damage, recruited to sites of double-strand breaks by the RPA complex (PubMed:26774285). Recruited to chromatin following DNA damage induced by UV irradiation, or camptothecin or hydroxyurea treatment (PubMed:22194613).

Diseases

Osteosarcoma
Extra Unidentified Structurally Abnormal Chromosome (disorder)
Arthritis
Edema
Infectious Mononucleosis
Chromosome Markers
Retroperitoneal Neoplasms
Liposarcoma

Rheumatoid Arthritis
Synovitis
Physiological Stress

Pathways

Dna Replication
S Phase
Single-stranded Dna Binding
Cell Cycle

PTMs

Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/HELB

References

PMID: 12181327 by Taneja P., et al. A dominant-negative mutant of human DNA helicase B blocks the onset of chromosomal DNA replication.

PMID: 15146062 by Gu J., et al. Cell cycle-dependent regulation of a human DNA helicase that localizes in DNA damage foci.