Histone H3.3 Antibodies

AND H3-3A ELISA kits, Histone H3.3 Proteins

Many biological assays use antibodies to detect Histone H3.3, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Histone H3.3 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Histone H3.3 antibodies...

We validate the specificity of these antibodies to Histone H3.3 by testing them on tissues known to express H3-3A positively and negatively. Browse below to find the H3-3A antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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H3-3A Infographic by Boster Bio

All H3-3A Products

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Product

Figures

Specificity

Applications

Price

Human H3F3A Recombinant Protein

SDS-PAGE, Denatured

$250
Cat # PROTP84243
Starting from 20ug

Anti-H3f3b Antibody (C-Term)

Mouse

$299
Cat # M06819-30
50 µl

Anti-Phospho-Histone H3.3 (S31) Rabbit Monoclonal Antibody

Human, Mouse, Rat

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # P06819-5
100 μl/vial

H3.3A (H3F3A) (NM_002107) Human Over-expression Lysate

Human

$628
Cat # LS003020
100 µg

H3-3A Overview

Facts about Histone H3.3.

H3-3A 3D structure. Source: alphafold

Histone H3.3 (H3-3A)

Variant histone H3 which replaces conventional H3 in a broad assortment of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis.

Deposited at websites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, restricting DNA accessibility to the cellular machineries which require DNA as a template.

Gene Information

Human
Gene Name:	H3-3A
Uniprot:	P84243
Entrez:	3020

Family

Belongs to:

histone H3 family

Alternative Names

Histone H3.3

Molecular Weight

Mass (kDA):
15.328 kDA

Genomic Context


Human

Location:	1q42.12
Sequence:	1; NC_000001.11 (226062716..226072019)

Subcellular Localizations

Nucleus. Chromosome.

Diseases

Glioma (GLM)

Interacting Proteins

ASF1B
CBX5
CHAF1A
DAXX
DNMT3A

HIST1H4A
PELP1
TAF3
Zmynd11

PTMs

Acetylation
ADP-ribosylation
Citrullination

Hydroxylation
Methylation
Phosphoprotein

Ubl conjugation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/H3-3A

References

PMID: 2859593 by Wells D., et al. Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs.

PMID: 3031613 by Wells D., et al. Unusual structure, evolutionary conservation of non-coding sequences and numerous pseudogenes characterize the human H3.3 histone multigene family.