GFRA3 Antibodies

GDNF family receptor alpha-3 (GFRA3)

Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.

.

Gene Information

Human
Gene Name:	GFRA3
Uniprot:	O60609
Entrez:	2676

Family

Belongs to:
GDNFR family

Alternative Names

GDNF family receptor alpha 3; GDNF family receptor alpha-3; GDNF R alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR alpha3; GFR alpha-3; GFRA3; GFRa-3; GFR-alpha-3; glial cell line-derived neurotrophic factor receptor alpha-3; GPI-linked receptor

Molecular Weight

Mass (kDA):
44.511 kDA

Genomic Context

Human
Location:	5q31.2
Sequence:	5; NC_000005.10 (138252380..138274621, complement)

Tissue Specificity

Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system.

Subcellular Localizations

Cell membrane; Lipid-anchor, GPI-anchor.

Diseases

• Nervousness
• Carcinoma
• Neoplasms
• Neoplasm Invasiveness
• Pain
• Medullary Carcinoma Of Thyroid
• Thyroid Neoplasm
• Cell Invasion
• Nerve Damage
• Malignant Neoplasm Of Thyroid
• Neoplasm Metastasis
• Brain Ischemia
• Malignant Neoplasms

• Congenital Absence
• Ischemia
• Malignant Paraganglionic Neoplasm
• Hyperalgesia
• Peripheral Neuropathy
• Neurodegenerative Disorders
• Numbness

Interacting Proteins

• ARTN

Pathways

• Localization
• Transport
• Aging
• Hypersensitivity
• System Development
• Cell Proliferation
• Regeneration
• Nervous System Development
• Innervation
• Brain Development
• Neurogenesis
• Reverse Transcription
• Swimming

• Mrna Transcription
• Translation
• Blastocyst Development
• Peripheral Nervous System Development
• Peyer's Patch Development
• Enteric Nervous System Development
• Glycosylation

PTMs

• Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GFRA3

Best Uses Of The GFRA3 Marker From Boster Bio

This article will explore the GFRA3 Marker from Boster Bio. During the course of the article, you'll learn about high-affinity primary antibodies, Mouse and rabbit models, and GFRA3 markers. In addition, we'll discuss how Boster Bio has developed their own GFRA3 antibodies. If you're looking to use GFRA3 in your research, this article is for you!

Boster Bio

The GFRA3 marker is a key component in many biological assays. Boster has developed a number of antibodies that can detect this marker. These antibodies are monoclonal or multiclonal, and can react with GFRA3 when tested in animal samples. They have also been validated on Western Blotting, Immunohistochemistry, and ELISA.

GFRA3 markers

GFRA3 is an important component of several biological assays. It is detected in antibodies that are monoclonal, polyclonal, or monoclonal. These antibodies react with the protein of many animal samples. Boster Bio uses rabbit and mouse to develop GFRA3 antibodies. The antibodies have been validated for use in Western Blotting and Immunohistochemistry. Boster Bio provides GFRA3 markers that enable researchers to determine GFRA3 expression within various tissues.

Primary antibodies of high affinity

Functional selection of high affinity primary antibodies is a highly effective strategy to identify single domain antigens that bind specifically to proteins in the central nervous systems. Bivalent Fc fusion protein, for instance, is capable of carrying biotherapeutics. It can be bound to many biomolecules. This approach could revolutionize the development biotherapeutics.

Nano-Secondaries may also be monovalent and bind specifically to target IgGs. They are subclass-specific as well as high affinity. Nano-Secondaries are 10 times smaller than conventional secondary antibody. Their size allows for better tissue penetration and decreases the distance between the epitope and the label. Immunostaining HeLa cells with anti-bActin antibody or rabbit anti-LaminB1 antibody helped to identify the GFRA3 marker.

In one study, mice were injected with TXB2-hFc or 8D3-hFc at 25 or 250 nmol/kg. Blood samples were collected after 18 hours. PBS-injected animals were used as controls. The samples were diluted one-to-five in PBS and added to 1 ml of BD Retic-Count reagent (BD Biosciences). The samples were incubated 60 minutes at RT.

Next came the identification of the antibody that binds the epitope to the fixed tissue. An immunohistological assay was used to identify the epitope. A fluorescent substrate was used to visualize the antibodies. This step allows simultaneous identification and quantification of 61 different antigens within the same tissue section. These results are often used to compare markers from different tissues.

TXB2-hFc antibodies bind to TfR1 soluble ECDs in rat and cynomolgus monkey cells. The bio-Tf binding did not interfere with TfR1 in the brain. TXB2-hFc binds to TfR1 and fails to penetrate the BBB. TXB2-hFc was not comparable to TXB2-hFc tested in the mouse's brain.

Models of mouse and rabbit

In the CNS of mice, GFRA3 (a novel gene) was identified. It is found in the cingulate and hippocampus. It has many biological functions, including mechano and chemical sensing, thermoreception and proprioception. Earlier research has used this gene as a surrogate marker for neuron clusters. A further gene known as DLK1 is expressed in the CNS. It is also expressed within the kidneys.

We can confirm the expressions of GFRA3 within neurons in the C1-C4-C5-C8 regions using single-cell realtime PCR. We can also use the GFRA3 marker for examining the functional effects of GFRA3 in the development of the central nerve system in humans. This gene co-expressed the markers Gal (Nppb), Th, Gfra2, S100b and Gfra2, which were previously identified as part of the C1-C8–C9 brain.

Developing GFRA3 antibodies

GFRA3 has been identified as a common target in biological assays. To detect the protein, antibodies react to it in many animal samples. Boster Bio develops antibodies against GFRA using rabbit and mouse.