GRHL1 Antibodies

Grainyhead-like protein 1 homolog (GRHL1)

Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' (PubMed:12175488, PubMed:18288204, PubMed:29309642).

Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier. There is no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development (By similarity).

Gene Information

Human
Gene Name:	GRHL1
Uniprot:	Q9NZI5
Entrez:	29841

Family

Belongs to:
grh/CP2 family

Alternative Names

grainyhead-like 1 (Drosophila); grainyhead-like protein 1 homolog; LBP protein 32; MGRLBP-32; NH32; TFCP2L2; Transcription factor CP2-like 2LBP32Mammalian grainyhead; Transcription factor LBP-32

Molecular Weight

Mass (kDA):
70.113 kDA

Genomic Context

Human
Location:	2p25.1
Sequence:	2; NC_000002.12 (9951693..10002279)

Tissue Specificity

Isoform 1 is highly expressed in brain, pancreas, tonsil, placenta and kidney. Isoform 2 is highly expressed in brain and liver. Expressed at very low levels in non- steroidogenic cells.

Subcellular Localizations

Nucleus.

Diseases

• Tissue Adhesions
• Exencephaly
• Palmoplantar Keratosis
• Flushing
• Neoplasms
• Spina Bifida
• Gastroesophageal Reflux Disease

• Barrett Esophagus
• Choriocarcinoma

Pathways

• Neural Tube Closure
• Cell Proliferation
• Localization
• Tube Closure
• Cell Cycle
• Wound Healing
• Transport
• Cell Adhesion
• Lipid Localization
• Endoderm Development
• Organic Acid Transport
• Dorsal Closure
• Dna Repair

• Ovulation
• Protein Oligomerization
• Embryo Development
• Regulation Of Dna Binding
• Protein Folding
• Placenta Development
• Cell Morphogenesis Involved In Differentiation

PTMs

• Cleavage

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GRHL1

Best Uses of the GRHL1 Marker

The GRHL1 marker is a protein coding gene that is part of the grainyhead family of transcription factors. Boster Bio offers the GRHL1 marker for IHC and ELISA. In this article, we'll take a look at the most effective applications of this marker for diagnostics. We'll also talk about the differences between the GRHL1 marker and other markers of gene expression.

GRHL1 is a member of the grainyhead family of transcription factors.

The GRHL1 gene is a crucial regulator protein, plays a role for the development of both plant and animal cells. Numerous diseases are linked to this gene, including Deafness, Autosomal Dominant 28 and Maxillary Cancer. In addition, activating metabolism and gene expression is also part of the PPARA. Annotations for the GRHL1 gene include the transcription factor that binds DNA and RNA Polymerase II cis regulatory region-specific DNA binding.

Researchers studied the genetic makeup of Mexican and Chinese populations in the book "Best uses for the GRHL1 marker". Many researchers used diverse data to base their findings. The publication was co-authored by scientists from China, Mexico, and the United States. Other authors of the book include Davalos, Albert; Witts, Emily C.; Miles, Gareth B. and Dholakia, Kishan.

It is a protein coding gene

The GRHL1 and GRHL3 genes are implicated in many types of cancer. Grhl1-GRHL3 mice are more likely to develop skin carcinomas than their wild type littermates. The mice that are knocked out of conditional Grhl3 exhibit similar traits. Additionally, they have an increased risk of developing other types of cancer. Despite the many benefits of targeting these genes they are largely unexplored and therefore require further research.

Clinical studies have proven that ESCC has low levels of GRHL1. However, ESCC has shown that GRHL1 protein expression is decreased. This is linked to lower outcomes. Further research should investigate the role of GRHL1 in ESCC to devise individualized treatments. It is also essential to determine the precise molecular mechanisms involved in GRHL1 regulation of tumor growth.

Although little research has been conducted into the role of GRHL1 for various types of cancer, this protein-coding genes acts as a tumor-silence genes by stopping the growth and clone formation in cancer cells in mice. Knockout mice also had larger tumors and higher rates of ESCC. GRHL1 could be a potential target gene for treating ESCC.

It is used in ELISA as a marker

The human gene GRHL1 (proto-oncogenic transcription factor) is an important regulator of insulin signaling as well as long-lived. Its interaction with other transcription factors could be an important factor in the creation of geroprotective drugs. It is also involved in various physiological processes for instance, the maintenance of Telomeres. The interaction of GRHL1 with other proteins is described in the human GRHL1 protein interactome.

GRHL1 is a key regulator of glucose homeostasis and insulin signaling in humans. It is a conserved gene from nematodes to mammals and is a crucial factor in the regulation of longevity. The blood glucose levels of fasting can alter the expression of the gene. It is also implicated in the insulin/IGF-1 signaling. This means that GRHL1 can be used as an indicator for ELISA.

The GRHL1 transcription factor has been implicated in skin cancer development. Chemical carcinogenesis in mice has shown that it is a contributor to the development of skin tumors. The current study shows that mice with Grhl1 don't develop tumors spontaneously. They are similar to their wild-type littermates. The Grhl1mice have the same timing for the development of papillomas in chemical carcinogenesis.

GRHL1 can be used to measure EGFR activity. This gene is extremely sensitive and specific. This marker is particularly helpful in human cancer research since it is widely used in ELISAs and can be a helpful diagnostic tool to determine tumor occurrence. There are more than 30 human cancers whose cells lack GRHL1 expression.

It is used in IHC

GRHL1 is a transcription factor that regulates the expression of various genes that are involved in airway epithelial cells' functions. It is found in many tissues, including the airway epithelium in which it plays a part in regulating cell morphogenesis, adhesion, as well as motility. However, there is a lack of information about its function in human diseases. In this study, we employed an animal model of COPD to determine GRHL1 expression in human lung tissues.

GRHL1 is present in a variety of tissues in the body, including the skin. The high expression of GRHL1 in tumors significantly slows tumor development and decreases foci formation. A lower rate of cell expansion was also observed when GRHL1 was expressed in human skin tumors. These results suggest that GRHL1 could be a biomarker for various diseases.

While ESCC patients have seen improved clinical outcomes, there are significant differences between patients with the same clinical stage. Finding new biomarkers can aid in the diagnosis of ESCC and assist in determining the best treatment. GRHL1 is reduced in ESCC, both at the mRNA and protein level and the absence of GRHL1 in ESCC is associated with a less favorable prognosis.

It is used in ICC

GRHL1 is a crucial co-translational factor, and its activation is essential for enhancer activity. Inhibition of this gene might limit the proliferation of cancerous cells in the colon. This study focuses on human lung fibrosis in mice. Patients suffering from CC might benefit from a better outcome should they be able to block the GRHL genes. The research isn't sufficient to determine if the GRHL1 inhibitors are efficient.

It is used in immunofluorescence

The proteins GRHL1 and GRHL2 are crucial regulators of various target genes. When these proteins are mutated in human tumors, it is possible to stimulate the growth of cells. In immunofluorescence the antibodies to GRHL1 can be utilized. These antibodies are able to recognize proteins in living cells. This enzyme can be used by immunofluorescence to determine presence of a particular protein or molecule within the tumor.

MLE15 cells were cultivated on glass coverslips and fixed at 70-80% confluence. After this they were lysed using 1x PBS containing 4% formaldehyde. After blocking with 5 percent skimmed milk TBST for 30 minutes The cells were then incubated overnight with the rabbit anti-human GRHL1 monoclonal antibody (1:250). Secondary antibodies were used to determine the presence of GRHL1 protein.

The study showed that GRHL1 was significantly overexpressed in NSCLC tissues. The protein was also linked to the progress of the disease. In the tumor tissues, high levels GRHL1 were also found. The study suggested that GRHL1 could be a potential new therapeutic target for NSCLC. Further research is required to identify the mechanism of GRHL1 immunofluorescence.

Multiple genes are controlled by GRHL1. The GRHL1 gene is involved in the regulation of epidermal desmosomes. These genes control the differentiation of skin cells. Their expression in human and mouse skin cells could indicate a compromised skin barrier. This gene can be used in immunofluorescence to help identify the problem with the skin barrier.