GCLM Antibodies

Glutamate--cysteine ligase regulatory subunit (GCLM)

Gene Information

Human
Gene Name:	GCLM
Uniprot:	P48507
Entrez:	2730

Family

Belongs to:
aldo/keto reductase family

Alternative Names

Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; GCS light chain; GLCLRglutamate--cysteine ligase regulatory subunit; glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD); Glutamate--cysteine ligase modifier subunit; glutamate-cysteine ligase regulatory protein; glutamate-cysteine ligase, modifier subunit; GSC light chain

Molecular Weight

Mass (kDA):
30.727 kDA

Genomic Context

Human
Location:	1p22.1
Sequence:	1; NC_000001.11 (93885199..93910370, complement)

Tissue Specificity

In all tissues examined. Highest levels in skeletal muscle.

Diseases

• Liver Carcinoma
• Malignant Neoplasms
• Schizophrenia
• Inflammation
• Liver Neoplasms
• Neoplasms
• Myocardial Infarction
• Neurotoxicity Syndromes
• Hepatotoxicity
• Mental Disorders
• Infarction
• Lung Diseases
• Neurodegenerative Disorders

• Malnutrition
• Malignant Neoplasm Of Lung
• Injury To Liver
• Dysequilibrium Syndrome
• Atherosclerosis
• Lung Diseases, Obstructive
• Fibrosis

Pathways

• Cell Death
• Pathogenesis
• Cell Proliferation
• Cell Cycle
• Conjugation
• Localization
• Aging
• Cell Growth
• Response To Oxidative Stress
• Inflammatory Response
• Sensitization
• Prepulse Inhibition
• Proestrus

• Gastric Emptying
• Transport
• Cellular Homeostasis
• Immune Response
• Regulation Of Gene Expression
• Cell Cycle Arrest
• Innate Immune Response

PTMs

• Phosphorylation
• Reduction
• Cleavage

• Oxidation
• Glutathionylation
• Methylation

• Myristoylation

Research Areas

• Cancer

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GCLM

Best Uses Of The GCLM Marker

Steven Boster, the founder of GCLM Marker in 1993, developed the first IHC product. Boster created hundreds of primary antibody products and quickly became China's largest catalogue antibody company. PicoKine(tm), Boster's own proprietary ELISA technology, was created by him in the late 1990s. PicoKine uses trade secrets to deliver high-sensitivity ELISA kit for molecular biology.

Steven Boster

Steve Boster was a retired sales manager and member of Concordia Hall of Staunton, VA. He died June 26, 2022. He was a retired sales manager and member of Concordia Hall of Staunton, VA. His two daughters, Natosha and Crystal Peck, are his survivors. His 4 Brothers Jack, Sr. as well as Sandra Blanton, are also his survivors. His sister Lisa Milton, along with many nieces or nephews, is his survivor.

Dr. Steve Boster is a native of San Angelo Texas. He grew up on a small hobby farm near Mora. He graduated from the University of Minnesota College of Veterinary Medicine with a veterinary degree in 1990. He began his career in Cold Spring and Sauk Center before joining Buffalo Equine. In 2004, he was a partner in the practice. Steve loved hunting and fishing, and donated a percentage of the sale price of his paintings to the Fisher House Foundation.

Primary antibodies of high-affinity

Abcam produces RabMAbs. These antibodies' KD values were derived using published literature. However, they may have been made using different methods. These antibodies were measured using KD as part of the Ol RD measurement project. These antibodies are more affinity than mouse monoclonal antibody. Abcam is proud that the GCLM marker has been released and the high-affinity primaries have been released.

GCL, and GCLC make up GCLM. Its function is to catalyze the oxidative stress-induced conversion of GSH to glutathione. GCL activity was increased under oxidative stresses in human lymphocytes. However, there was no change in GCL proteins. These alterations resulted in a shift in GCL activity, from monomeric to holoenzyme forms.

Their molecular weights influence the affinity between GCLC or GCLM. The monomer GCLM is less than half of total pool. H2O2 treatment and phorone treatment induced shifts in the protein to a 100-kDa form, and both treatments promote the formation of GCLM and GCLC oligomers.

GCL's activity is higher in heterodimeric GCL than monomeric GCL. The complete shift to native PAGE of the lower mobility form is a sign that heterodimerization has occurred in GCLC or GCLM. After this, samples were subjected either to native or SDS-PAGE. The top, bottom and middle panels show immunoblots GCLC/GCLM compared to GCLM. In addition, the top panel and bottom panels display Coomassie staining of native PAGE gels corresponding proteins.

IgD is necessary for the production of high-affinity IgM. However, this protein cannot not be produced without IgD. Lacking IgD results in the generation of autoreactive IgM antibodies and autoimmune diseases. Both WT and IgD-deficient mice exhibited signs of diabetes and showed symptoms similar to other types of autoimmunity.

These monoclonal monoclonal immune cells were generated using polyclonal polyclonal antisera from rabbit polyclonal. The selection of the peptide antigens was made based on homology between amino acid sequences and presence of hydrophilic residues. The same antigen was then repeated inoculated to the rabbits. Western blots of serially diluted antibodies were used to characterize them.

In vivo the IgD–class BCR regulates antigen-valence and antibody secretion. Monovalent antigens inhibit IgG production and promote the production of high-affinity IgM antibody. Polyvalent antigens on the other side induce IgG memory reactions in activated mature B cell. In maintaining the quality and longevity of their immune responses, it is important that B cells are controlled by IgD.

Secondary antibodies can be conjugated with the target antigen or unconjugated. The application and detection method will determine the label choice. Common enzymes used for this purpose are alkalinephosphatase or horseradish peroxidase. Both enzymes produce longer-lasting signals. While HRP is less expensive, it's also less stable. Phosphorase is more sensitive, lasts longer, and is therefore more stable.

GCLM Marker

GCLM has many uses. It can be found in many human tissues. The cells of the brain, and spleen were shown to have GCLM. GCLM expression was also found in the brains and spleens of animals. Both normal basal cells as well as tumors showed similar levels of GCLM expression in these organs. It is not yet known how the gene functions in humans.

GCLM plays a vital role in the survival and function of erythrocytes. In the presence or oxidative strain, a deficiency of this protein can result in increased erythrocyte damages. Thus, mice lacking this gene have increased erythrocyte damage. These damaged cells build up in the spleen. This gene has many uses and is currently under extensive research.