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- Table of Contents
Facts about Glucokinase regulatory protein.
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Human | |
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Gene Name: | GCKR |
Uniprot: | Q14397 |
Entrez: | 2646 |
Belongs to: |
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GCKR family |
FGQTL5; GKRP; glucokinase (hexokinase 4) regulator; glucokinase (hexokinase 4) regulatory protein; Glucokinase regulator; glucokinase regulatory protein
Mass (kDA):
68.685 kDA
Human | |
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Location: | 2p23.3 |
Sequence: | 2; NC_000002.12 (27496839..27523689) |
Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.
Cytoplasm. Nucleus. Mitochondrion. Under low glucose concentrations, GCKR associates with GCK and the inactive complex is recruited to the hepatocyte nucleus.
If you're interested in the most effective treatment for diabetes, high-affinity primary antibodies to the GCKR Marker are the answer. These antibodies are highly specific and effective against a range of cell types and tumors. These antibodies are based on Steven Boster's breakthrough technologies. Here's what you should know about them. They are also applicable to scientists from around the world.
Primary antibodies are produced by immunizing animals with a synthetic peptide that corresponds to the amino terminus of the human GCKR protein. The monoclonal antibodies used in immunohistochemistry have very high affinity for the GCKR protein. Because their affinity is high, they may require harsher methods for elution. However, these high-affinity antibodies can still be useful in immunohistochemistry.
The protein encoded by the GCKR gene is an inhibitor of the enzyme glucokinase in the liver and pancreatic islet cells. The protein forms an inactive complex with the enzyme, and is considered a susceptibility gene for MODY. This gene has been recommended for WB detection because of its role in the regulation of the production and transport of glucose in the body. Molecular analysis of immunogens can help to identify high-affinity primary antibodies against the GCKR Marker.
Affinity is the strength of binding between a monoclonal antibody and its ligand. It is measured using the equilibrium dissociation constant (KD) of the two molecules. The process of affinity binding is reversible, with the rate of bind equal to the rate of dissociation of the reactants. A KD value is a measure of the affinity of the antibody. The lower the KD, the higher its affinity.
The IgD BCR regulates antibody secretion in vivo by sensing antigen-valency. A monovalent antigen inhibits the production of high-affinity IgG antibodies, while a polyvalent antigen stimulates the generation of highly specific IgM antibodies. When mice are lacking IgD, they develop high-affinity antibodies against dsDNA.
The ability to visualize two antigens on the same tissue section is critical for successful immunohistochemistry. The primary antibody, while binding to GCKR, must not cross-react with secondary antibodies. The secondary antibody, meanwhile, must also be monomeric in order to prevent passive capture of the secondary antibody by the primary antibody. The two-antibody system is an invaluable tool in immunohistochemistry.
The rabbit antibody repertoire has long fascinated immunologists. It is easy to obtain and contains both allotypes and genetic variants. The studies of rabbit antibodies led to initial descriptions of allelic exclusion, neonatal suppression of serum IgG production, and the discovery of V and C region genes that are separately encoded. These antibodies are highly sensitive to changes in repertoires at the population level.
If you're interested in researching the effects of the GCKR gene on cell growth, then you've come to the right place. Boster offers high-affinity primary antibodies that have been validated and cited in the scientific literature. The GCKR gene is one of the most important genes for the development of insulin-resistant diabetes and is associated with a higher blood glucose level.
PMID: 8589523 by Warner J.P., et al. Human glucokinase regulatory protein (GCKR): cDNA and genomic cloning, complete primary structure, and chromosomal localization.
PMID: 9570959 by Hayward B.E., et al. Organization of the human glucokinase regulator gene GCKR.