EIF2S1 Antibodies

Eukaryotic translation initiation factor 2 subunit 1 (EIF2S1)

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex.

Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and launch of an eIF-2- GDP binary complex. For eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Gene Information

Human
Gene Name:	EIF2S1
Uniprot:	P05198
Entrez:	1965

Family

Belongs to:
eIF-2-alpha family

Alternative Names

eIF2 alpha; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; EIF2S1

Molecular Weight

Mass (kDA):
36.112 kDA

Genomic Context

Human
Location:	14q23.3
Sequence:	14; NC_000014.9 (67360328..67386516)

Subcellular Localizations

Cytoplasm, Stress granule. Colocalizes with NANOS3 in the stress granules.

Diseases

• Diabetes Mellitus, Non-insulin-dependent
• Obesity
• Hyperglycemia
• Pathological Dilatation
• Impaired Glucose Tolerance
• Diabetes Mellitus

Interacting Proteins

• ACTN1
• DDX3X
• EIF2AK2
• Eif2ak3
• EIF2S2

• EIF2S3

Pathways

• Translation
• Glucose Homeostasis
• Cell Cycle
• Insulin Secretion
• Secretion

PTMs

• Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/EIF2S1

Best Uses Of The EIF2S1 Marker

You have come to the right place if your goal is to find the best anti eIF2S1 protein. Our Anti eIF2S1 Alpha (2E4) antibodies reacts with Mouse and Rat as well as Human. Continue reading to learn more about the anti-eIF2S1 anti-eIF2S1 antibodies. The bosterbio eif2s1 antibodies catalog number can found here.

Anti-eIF2 Alpha (2E4) monoclonal EIF2S1 Marker

The EIF2alpha monoclonal eIF2 proteins marker is a high quality monoclonal eIF2alpha antibody. This antibody is specific for eIF2 alpha proteins of human, mouse, and rat origin. It is available in several combinations. This protein interacts with RNA molecules, and proteins called protein initiators, during the process of synthesis.

The integrated stress response is incomplete without eIF2S1/eIF-2alpha. It is required to adapt to different types of stress. This protein's phosphate is controlled by metabolic stress sensing protein kinases like EIF2AK1/HRI or PKR. The protein interacts with transcription activators like QRICh2.

eIF2a is heterotrimer and consists of three units. It drives the binding of the initiator, methionyl–tRNA, to the 40S Subunit of the ribosomal. eIF2a can be phosphorylated with related proteins to inhibit its activity and prevent guanine nocletide exchange. It is only legal for researchers to use this marker.

Applications

The EIF2S1 indicator can be used to detect eIF2B phosphorylation. They are both important components of an integrated stress response. The protein kinases EIF2AK1/HRI and EIF2AK2/PKR phosphorylate the protein, which is responsible for regulating various metabolic processes in cells. EIF2AK4 is a transcriptional activator. QRICh2 is a transcriptional activator.

To detect the EIF2S1 gene, immunohistochemistry was applied to tissue samples from 145 ITACs. Immunohistochemistry, which used antibodies against EIF2S1, EIF5A or EIF6 was performed using an optimized protocol. The R functions'survcorr 'and 'pchisq'were used to calculate the respective p values. In all four groups, gene expression levels were statistically significant.

The protein EIF2S1 is part of the eIF2 complex and promotes the binding of initiator tRNA to the 40S ribosomal subunits. The phosphorylation state of EIF2S1 regulates the rate at which tRNA is translated. The protein is stabilized and slows down translation by phosphorylation by kinases. The recombinant human EIF2S1 marker was purified using conventional chromatography methods.

Incubation for blots containing antibodies

For immunodetection, blots are incubated with a dilute solution of the antibody overnight. The antibody dilution should be determined by the manufacturer's datasheet. To determine the antibody content, dot, slot, or testblots can all be used. The volume of immunodetection must be sufficient. The blot must be gently agitated to keep it evenly exposed to the antibody.

The second antibody conjugated is also used. The reporter molecules are mixed with hapten. This allows for quantification. The hapten binds to the reporter molecule and can be detected by looking at its signal. The result can be either quantitative or qualitative. The second antibody is linked to a reporter molecule, which indicates whether it has bound the hapten.

Secondary antibodies were dilute 1:5000 with buffer B, which contained 1% W/V BSA and 0.5% Teen-20. The blots were then washed according to the instructions. The appropriate wavelengths of light were used to detect the bound antibodies. Typically, fluorescent-labeled antibodies bind to first antibody-hapten complex. After that, the unbound reagent must be washed out.