ETV4 Antibodies

ETS translocation variant 4 (ETV4)

Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. .

Gene Information

Human
Gene Name:	ETV4
Uniprot:	P43268
Entrez:	2118

Family

Belongs to:
ETS family

Alternative Names

Adenovirus E1A enhancer-binding protein; E1A-FE1A enhancer binding protein; E1AFETS translocation variant 4; ets variant 4; ets variant gene 4 (E1A enhancer binding protein, E1AF); ets variant gene 4 (E1A enhancer-binding protein, E1AF); EWS protein/E1A enhancer binding protein chimera; PEA3; PEAS3; Polyomavirus enhancer activator 3 homolog; polyomavirus enhancer activator-3; Protein PEA3

Molecular Weight

Mass (kDA):
53.938 kDA

Genomic Context

Human
Location:	17q21.31
Sequence:	17; NC_000017.11 (43527843..43546340, complement)

Subcellular Localizations

Nucleus.

Diseases

• Malignant Neoplasms
• Neoplasms
• Malignant Neoplasm Of Prostate
• Prostatic Neoplasms
• Neoplasm Metastasis
• Cell Invasion
• Carcinoma
• Neoplasm Invasiveness
• Adenocarcinoma
• Malignant Paraganglionic Neoplasm
• Chromosomal Translocation
• Cell Transformation, Neoplastic
• Sarcoma

• Ewings Sarcoma-primitive Neuroectodermal Tumor (pnet)
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Carcinogenesis
• Tumor Progression
• Colorectal Cancer
• Colorectal Neoplasms

Pathways

• Cell Cycle
• Cell Proliferation
• Kidney Development
• Reverse Transcription
• Cell Motility
• Pathogenesis
• Cell Migration
• Limb Development
• Rna Interference
• Epithelial Cell Proliferation
• Oncogenesis
• Ovulation
• Localization

• Methylation
• Tissue Remodeling
• Cell Growth
• Cell Adhesion
• Fertilization
• Spermatogenesis
• Cell Cycle Arrest

PTMs

• Phosphorylation
• Methylation
• Ubiquitination

• Sumoylation
• Reduction
• Acetylation

Research Areas

• Cancer

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ETV4

Best Uses Of The ETV4 Marker

The Boster biomarker is a versatile reagent that can be used to study ETV4 markers. Boster scientists can submit results on species, applications, or special samples to receive product credits. This marker is accessible to scientists worldwide. Here are some of the best uses for this marker. You can find out more about this boster biomarker by clicking here. Learn more about the history of ETV4 genes.

Gene infographics

Boster's Gene infographics offer a visual explanation of all the human and mouse genes that comprise a human body. The gene infographics cover all of the human and mouse genes. You can also use the gene search bar to quickly access the gene that you're searching for. The infographics are organized by gene type so that you can quickly find the gene you are interested in.

Primary antibodies with high-affinity

The production high-affinity primaries antibodies is a process where the humoral immunity system generates a variety of proteins that can recognize nearly any antigen. Antigen affinity purification is a process that selects these antibodies from the repertoire. This process involves the passage of polyclonal antibodies through an affinity column that contains an immobilized antigen. Non-specific antibodies can be eluted, while antigen-specific antibody are retained.

The principle of affinity-maturing antibodies rests on the observation of the CDR of unbound germ line precursors adopting a conformation nearly identical to that found in mature CH58. Preorganization leads to an increase in affinity during maturation and reduces complex formation's entropy cost. It also results in a 2,000fold increase in affinity. This process was also applied to the CH65 anti-influenza virus HA antibody lineage.

You can purchase primary antibodies from many commercial sources. You should be aware that the quality and availability of these antibodies may vary from one supplier. It is recommended that you verify the quality of any antibody before purchasing. R&D Systems can be contacted to order your antibody directly. This will ensure you control the commercial source. If you don’t receive satisfactory results, you can always get assistance.

An additional interaction with the antigen is another example of affinity maturation. The germ line precursor to antibody CH58 interacts, for example, with the V2 protein of HIV-1 envelope glycoprotein. This accession code is 4RIS. The resulting antibody inter-reacting intermediates could cross-react against Env. Recent research also showed that affinity maturation can also occur through the formation of two salt bridges.

A tissue microarray of 282 cases invasive breast carcinoma was done to detect the presence high-affinity primary antibodies for ETV4. TMA sections were stained using the rabbit polyclonal anti-rabbit antibody. The percentage of positive cells was determined after determining the intensity of ETV4 staining. The ETV4 antigen in PC3 cells containing an ETV4 variant was significantly higher than that found in normal cells.

Another study involving high-affinity primary antibodies using the ETV4, a protein found in mice, revealed that these proteins had different KD distributions. KD values were derived based on published literature, and could have been obtained using different methods. However, RabMAb KD numbers were derived by the Ol-RD measurement program and independently verified in Abcam labs. The differences in affinity/sensitivity between RabMAbs and mouse MAbs suggests that the HIFa degradation pathway may become saturated when these proteins have been overexpressed.

The next step in producing high-affinity secondary antibodies using the ETV4 marker is the preparation. These antibodies are usually conjugated with several labels. The type of secondary antibody you choose will depend on what you are testing for and what your downstream application is. Your research will be significantly faster if high-affinity antibodies are used with the ETV4 markers. It is important to make sure the antibody you choose is compatible.

Histological background

The Histological background of Boster is somewhat speculative, but there is some evidence that the viral infection is widespread in the ileum and bowel. Boster AR0022 reagent could be used to treat tissue or organ sections. Three percent H2O2 mixed with methanol was used by endogenous antioxidants to stop them from multiplying. Although the virus infection is rare in humans, it is still a concern.