ESRRG Antibodies

Estrogen-related receptor gamma (ESRRG)

Orphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements (By similarity).

Induces the expression of PERM1 in the skeletal muscle. .

Gene Information

Human
Gene Name:	ESRRG
Uniprot:	P62508
Entrez:	2104

Family

Belongs to:
nuclear hormone receptor family

Alternative Names

DKFZp781L1617; ERR gamma; ERR gamma-2; ERR3; ERRG2; ERRgamma; ESRRG; Estrogen receptor-related protein 3; estrogen-related receptor gamma; FLJ16023; KIAA0832; NR3B3; Nuclear receptor subfamily 3 group B member 3

Molecular Weight

Mass (kDA):
51.306 kDA

Genomic Context

Human
Location:	1q41
Sequence:	1; NC_000001.11 (216503246..217137702, complement)

Tissue Specificity

Expressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland.

Subcellular Localizations

Nucleus.

Diseases

• Malignant Neoplasms
• Malignant Neoplasm Of Breast
• Neoplasms
• Mammary Neoplasms
• Diabetes Mellitus
• Adenocarcinoma
• Prostatic Neoplasms
• Malignant Neoplasm Of Prostate
• Obesity
• Carcinoma
• Cell Transformation, Neoplastic
• Hypoxia
• Pathologic Neovascularization

• Ischemia
• Nervousness
• Tumor Angiogenesis
• Ovarian Neoplasm
• Congenital Absence
• Congenital Abnormality
• Liver Carcinoma

Interacting Proteins

• ADCY4
• DEUP1
• KIFC3
• MEOX2
• NR0B1

• NUP160
• PPARGC1A
• SHTN1
• SUMO1P1

Pathways

• Transport
• Cell Proliferation
• Secretion
• Localization
• Energy Homeostasis
• Cell Growth
• Gluconeogenesis
• Angiogenesis
• Fatty Acid Oxidation
• Adiponectin Secretion
• Cell Activation
• Tricarboxylic Acid Cycle
• Osteoblast Differentiation

• Cardiac Muscle Contraction
• T Cell Activation
• Proteolysis
• Muscle Contraction
• Regulation Of Gene Expression
• B Cell Activation
• Carbohydrate Utilization

PTMs

• Oxidation
• Phosphorylation
• Acetylation

• Dephosphorylation
• Sumoylation
• Reduction

• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ESRRG

Boster Bio: Best Uses Of The ESRRG Marker

If you're looking for a novel way to measure the expression level of an epigenetic marker, you've come to the right place. This article will explain how to detect boster protein with the help of anti-DDK affinity column. Then, you'll be introduced to the benefits of Sophoridine an innovative inducer of ESRRG. Finally, we'll discuss the benefits of using ESRRG markers in your research.

Anti-ESRRG Monoclonal Antibody

To perform Flow Cytometry analysis on U20S cells the monoclonal rabbit anti ESRRG antibody was employed. The samples were subjected to a 37°C incubation for 30 minutes. In addition to this primary antibody, the scientists used a secondary anti-rabbit IgG (DyLight!AE488 conjugated) as well as a negative control.

The Anti-ESRRG Monoclonal produced by Boster Bio reacts with Human, Mouse, and Rat. This antibody was created against an synthetic peptide that recognizes amino acids 360-410 from human ESRRB. The antibody is available in PBS with 0.02 percent sodium azide. It is recommended to purchase blocking peptides which react with a certain length of the immunogen.

Boster Bio, Guangzhou China is the producer of the ESRRG monoclonal anti-body. Boster Bio conducts a range of tests to determine the effectiveness of the antibodies. Boster Bio's antibody has been recommended for research studies in digestive diseases including autoimmune disorders, as well as cancer. This antibody is extremely specific. If you need a particular antibody or a generalized monoclonal, Boster Bio has the most appropriate antibody to conduct your research.

The KU Leuven was part of the research team as were other researchers from the Vaccine Research Institute (VIC) and Orleans Regional Hospital. The study sought to determine the specificity of this antibody to the SARS-CoV-2 antibodies in blood samples from patients who have been infected the virus or those who have been vaccinated against it. The results were positive. In fact the antibody was able to kill more than 95% of viruses in only two studies!

Anti-ESRRG siRNA

The target gene for Anti-ESRRG siRNA is ESRRG. This gene is a member the nuclear receptors family of. It was recently discovered to be a tumor suppressor , as well as an intriguing therapeutic target for cancer. In addition to its role in the process of fatty acid oxidation ESRRG reduces cell growth and reverses the EMT process and influences the expression of E.cadherin in the skeletal muscle. It is also involved in the initiation and maintaining of osteoblast differentiation and bone formation.

Bostern Bio's Anti -ESRRG siRNA reduces ESRRG activity by regulating Sox2-K123R. This gene is responsible for the function and differentiation of ES cells. It is also vital to maintain neural stem and progenitor cells within adult nervous systems. Anti-ESRRG siRNA is highly effective in reducing the expression of ESRRG. It can also be used to decrease expression of self-renewal markers like ERGF or hTERA.

Boster's Anti-ESRRG antibody identifies ESRRG within a paraffin-embedded rat cardiac muscle section. The tissue was treated with an antigen retrieval process that was heated in citrate buffer. It was then blocked with 10 percent goat serum. Then, it was incubated for 30 minutes with rabbit anti-ESRRG polyclonal antibodies. Next, the membrane was examined using a goat anti-rabbit IgG–HRP secondary antibody at a concentration of 1:1000 in TBS-1.1%Tween. This signal was generated using the Tanon 5200 system. As expected, the specific band was identified at 51KD.

The Anti-ESRRG siRNA comes from the AML-12 gene's genome cells. It is a targetable method for a gene of interest to investigate its expression. It is available in a variety of sizes and can be transfected in a variety of ways. A range of protocols have been developed, but this one is suggested for use in cancer research. In addition to Anti-ESRRG siRNA there are other products available.

Sophoridine as an innovative stimulator of ESRRG

Sophoridine is a novel inhibitor of DNA damage repair blocking the expression of RAD51 and phosphorylated H2AX which are markers for unrepaired DNA damage and homologous repair. These inhibitors trigger cell cycle inhibition in the G2/M phase, which is consistent to the sophoridine's mechanism of action. In addition to its potent effects on cell expansion, sophoridine is an intriguing new inducer of ESRRG.

The anti-tumor properties of Sophoridine are due to its ability to change the microenvironment that is a part of the immune system of gastric cancer patients. It blocks the chemotaxis process by macrophages that are a part of the tumor, and hinders their ability to M2-polarize. This results in increased cell proliferation and cytotoxic effects of CD8+ T cell. This is a powerful therapy tool for many ailments, including cancer, heart disease, and viruses.

Sophoridine is a powerful anti-tumor agent in cancerous gastric cells. It blocks invasion, migration and colony formation. Sophoridine also inhibits the repair of DNA breaks that are double-stranded that enhance cisplatin's antitumor effects. Sophoridine has been proved to be a safe and effective anti-tumor medication.

Sophoridine inhibits migration and invasion of gastric cancer cells as well as SGC7901 cell lines. The compound blocks the epithelial-mesenchymal transition in gastric cancer cells. It also increases the expressions of E-cadherin, N-cadherin protein, and N-cadherin. In addition, it reduces the expression of snail and vimentin both of which are key to gastric cancer cell migration and invasion.

Benefits of using the ESRRG Marker

The ESRRG Marker is a valuable gene in cancer research. It functions as a negative regulator of the Wnt signaling pathway. It could therefore be a therapeutic target. ESRRG is a member the NR superfamily. It is found in the stomach and the brain. It is involved in the pathophysiology and progression of many cancers, including liver and prostate cancer.

The full-length cDNA fragment of the ESRRG Marker was cloned to the pCDHEF1-T2A -Puro to allow it to be utilized. This vector was purchased from Invitrogen, Mountain View, CA by SBI. To perform transfection the authors used Lipofectamine 3000. In addition, Lipofectamine 2000 was used. Follow the manufacturer's directions to transfect cells with the ESRRG cDNA.