EPHX2 Antibodies

Bifunctional epoxide hydrolase 2 (EPHX2)

Bifunctional enzyme (PubMed:12574510). The C-terminal domain has epoxide hydrolase activity and functions on epoxides (alkene oxides, oxiranes) and arene oxides (PubMed:12869654, PubMed:12574510, PubMed:22798687).

Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (By similarity). Additionally determines steady-state levels of physiological mediators (PubMed:12869654, PubMed:12574510, PubMed:22798687).

Gene Information

Human
Gene Name:	EPHX2
Uniprot:	P34913
Entrez:	2053

Family

Belongs to:
AB hydrolase superfamily

Alternative Names

Bifunctional Epoxide Hydrolase 2; CEH; Cytosolic epoxide hydrolase; EC 3.3.2.10; EPHX2; Epoxide Hydratase 2; Epoxide hydratase; Epoxide Hydrolase 2; epoxide hydrolase 2, cytoplasmic; Epoxide Hydrolase 2, Cytosolic; epoxide hydrolase, soluble; SEH; Soluble epoxide hydrolase

Molecular Weight

Mass (kDA):
62.616 kDA

Genomic Context

Human
Location:	8p21.2-p21.1
Sequence:	8; NC_000008.11 (27491143..27597559)

Subcellular Localizations

Cytoplasm. Peroxisome.

Diseases

• Cardiovascular Diseases
• Hypertensive Disease
• Inflammation
• Ischemia
• Cerebrovascular Accident
• Malignant Neoplasms
• Neoplasms
• Atherosclerosis
• Brain Ischemia
• Heart Diseases
• Ischemic Stroke
• Tumor Angiogenesis
• Infarction

• Carcinogenesis
• Hypertrophy
• Myocardial Ischemia
• Coronary Heart Disease
• Diabetes Mellitus
• Myocardial Reperfusion Injury
• Coronary Artery Disease

Pathways

• Pathogenesis
• Cell Death
• Angiogenesis
• Excretion
• Cell Proliferation
• Methylation
• Transport
• Dna Methylation
• Localization
• Inflammatory Response
• Glucose Homeostasis
• Cell Cycle
• Demethylation

• Cell Growth
• Rna Interference
• Secretion
• Muscle Cell Proliferation
• Dna Demethylation
• Tube Formation
• Smooth Muscle Cell Proliferation

PTMs

• Phosphorylation
• Methylation

• Demethylation
• Cleavage

• Oxidation

Research Areas

• Lipid and Metabolism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/EPHX2

Boster Bio - The Best Uses Of The EPHX2 Marker

Steven Boster's biography contains more details on this groundbreaking biomarker. This acclaimed scientist is a specialist in Histology and the most effective uses of the EPHX2 Marker and other related subjects. Learn about his latest discoveries and products. Take a look at histology tools as well as ELISA kits. Also the hEPHX2 expression Vector is available. This information is important for all scientists, from the simplest to the most complex.

Histology

Histological analysis of EPHX2 gene revealed that this transcription factor can perform cross-species roles, including its role in the development of heart disease. Further research is required to elucidate the mechanism of its action. The PLZF transcription repressor identified the polymorphisms in 255T>C (and 111G>A). These polymorphisms also activate TGIF, a TALE homeodomain-containing transcription factor that competes with retinoic acid pathway.

The polymorphism in Ephx2 is linked to severe proteinuria and is highly predictive of stroke. A better sign of risk for stroke is severe proteinuria prior to the time of onset of the disease. To estimate stroke risk in patients with different Ephx2 genotypes the authors relied on urinary levels of protein. However, further research is needed to prove this hypothesis in transgenic mice.

Histological studies of the EPHX2 gene have shown that it is expressed more in young mice than in older mice. Prenephritic mice were more likely to express the gene more than mice who were normal age. EPHX2 gene expression was not affected by disease stage or age. However, EPHX2 gene expression levels appear to increase in the glomeruli of pre-transplant recipients and elderly patients suffering from an nephritic disease stage.

The change in EPHX2 in the urinary tract of SLE patients and mice with normal kidneys suggested a role played by the EPHX2 gene in the pathology. Although the severity of proteinuria wasn't measured, the study suggested that altering the EPHX2 gene could improve kidney function and slow the progression of the disease. These findings suggest that EpFAs may play a part in human ailments.

ELISA kits

Boster Bio is a manufacturer of ELISA kits and research antibodies that specialize in the detection and analysis of biomarkers in cancer, neuroscience and development biology. Its kits provide picogram level sensitiveness and have been tested against over 250 tissues. The QC department has validated ELISA kits against superfamilies and similar immunogenic proteins. Each kit is tested to confirm its reproducibility and specificity. It carries a high level of precision.

The Human APOC3 ELISA Kit was used to determine total plasma APOC3 levels. Raybiotech claims a sensitivity level of 0.2 mg/ml, and an intra-assay variability between 6 and 7.7 percent and 7.7%, respectively. Boster Bio EPHX2 ELISA kits are suitable for both research and clinical use. The manufacturer recommends using a high-quality assay kit to test plasma APOC3 levels in blood of humans.

The EPHX2 ELISA kit can be used for studies on various cancer cells . It is available for research and diagnostic purposes. It uses high-affinity antibody that are specific to one part of the antigen that is targeted. The capture antibody binds the antibodies and then captures them. The result of this process is analyzed as fluorescence or optical density versus concentration in an sigmoidal shape. Boster Bio EPHX2 EISA kits offer the highest sensitivities, come with 20 years of experience in manufacturing, and employ proprietary methods.

The MBS2540325 kit offers excellent sensitivity and high reproducibility in the detection of native and soluble epoxide hydrolase. The ELISA kit includes the blocking peptide of the soluble Epoxidehydrlase (sEH). The peptide is generated by a process known as the enzymatic hydrolysis (or hydrolysis) of PHOME. This reaction is highly bright. It employs the wavelength of 330 nm for excitation and 465 nm to detect. This peptide has been validated against 100 human recombinant enzyme hydrolase tests.

hEPHX2 expression vector

The EPHX2 gene is a member of the TNFR superfamily and is involved in several cells' responses. It can be found in a variety of cell types, including monocytes and leukocytes. This protein is involved in the immune response. It triggers numerous downstream effects by binding to CD40L. For different cell types, CD40 regulates different gene expression patterns. Multiple pathways are activated by signal transduction by CD40 which includes Jak3/STAT3, Jak3/STAT3, and PI3K/Akt.

CD40

500 genes in HCC have been associated with the EPHX2 marker. Some of the MFs found to be enriched in this gene include coenzyme as well as iron ion binding as well as an oxidoreductase function on the CHOH group of donor molecules. EPHX2 has been linked to pathways that are involved in carbon metabolism and the Cacade of Coagulation and Complementation, valine, leucine , and isoleucine degradation, among others.

Researchers have also associated EPHX2 expression with prognosis in cancer patients. Patients suffering from HCC were identified to have an unfavorable prognosis, OS or recurrence free mortality (RFS) due to the expression of this gene. However, further studies are required to determine what role that EPHX2 plays in the development of cancer. Listed below are some of the most common uses of EPHX2's marker in the field of cancer.

The CYP2J2 gene rs2280275 had not been examined in DN patients. In this study, however, the EPHX2 gene rs751141 was found to be strongly associated with ACR and creatinine, as well as eGFR. These results are promising, but they should be confirmed with an even larger sample. Further research is required to understand the mechanisms behind the actions of EPHX2's allele rs751141A.

Inflammation, oxidative stress, and atherosclerosis might all be linked to the presence of EPHX2's soluble version. A number of studies have looked into the link between polymorphisms found in EPHX2 with endothelial dysfunction and CV disease in the diabetic population. Additionally, the gene rs751141 is linked to decreased the release of nitric oxygen in diabetics.

These results demonstrate the EPHX2 expression pattern in a tumor cell sample. This protein expression pattern was lower in tumors compared to normal tissues nearby. These results support the idea that EPHX2 reduces the growth of CRC. For this reason, EPHX2 might be useful in assessing inflammation associated with tumors and the progression of cancer. Although more research is required, its benefits are clear. What are the most effective uses of the EPHX2 Marker?