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- Table of Contents
1 Citations 4 Q&As
1 Citations
Facts about Endothelial PAS domain-containing protein 1.
Regulates the vascular endothelial growth factor (VEGF) expression and appears to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier.
Human | |
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Gene Name: | EPAS1 |
Uniprot: | Q99814 |
Entrez: | 2034 |
Belongs to: |
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No superfamily |
anti hif 2 alpha; HIF-2 alpha; Basic-helix-loop-helix-PAS protein MOP2; BHLHE73; Class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain protein 1; endothelial PAS domain-containing protein 1; EPAS1; EPAS-1; HIF 2A; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2 alpha; HIF-2 alpha; hif2a angiogenesis; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2
Mass (kDA):
96.459 kDA
Human | |
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Location: | 2p21 |
Sequence: | 2; NC_000002.12 (46297407..46386697) |
Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
Nucleus. Nucleus speckle. Colocalizes with HIF3A in the nucleus and speckles.
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The EPAS1 gene (also called HIF2A) is a part in the larger protein complex hypoxia-inducible factors (HIF). This complex is vital for the body's ability to adapt to low oxygen levels. It regulates many genes which control the production of red cells blood vessel formation, blood vessel formation and cell division. HIF also regulates the production and release of the hormone erythropoietin.
When preparing a new vaccine, you may be tempted to purchase an Anti-HIF-2-alpha/EPAS1 Marker at Boster Bio. This reagent interacts with HIF-2a, which is highly efficient in the production of immunogen. Its high affinity makes it possible to create high-quality Ab. However, it has its own limitations. It's not as specific as certain antibodies and requires additional studies.
The Anti-HIF-2-alpha/EPAS1 Marker at Boster Bio is a monoclonal antibody which recognizes HIF-2a/EPAS1 protein. It can be used for immunohistochemistry, flow cytometry and ELISA on paraffin-embedded tissue. It is designed to detect an 118 kDa band that is the HIF-2 alpha protein. It has been utilized in a variety of studies, such as Knockdown Validated and in vivo.
Hypoxia is a regular occurrence in solid tumors. In order to respond to this condition, HIF-2-alpha/EPAS1 is activated CBP/p300 in the nucleus. In this manner HIF-dependent transcription takes place. Proper RNA splicing is necessary for the hypoxia response. Additionally the p300/CBP interactions with HIF targets genes.
HIF transcription factors are vital elements of humoral reactions, which include inflammation and immune response. HIF-1a and HIF-2b are crucial for Tfh cell proliferation. They also boost CD154 expression. Depletion of HIF-1a results in diminished humoral immunity. Both HIF-1a as well as HIF-2b alter the function of both CD4+ T cells as well as activated B cells.
HIF-2a is a vital HIF homolog, is vital for both genes. In mice the condition of pulmonary arterial hypertension can be promoted by a deficiency of HIF-2a. The loss of HIF-2a from the endothelial cells in mice results in diminution of aSMA cells which is essential in the progression and development of the disease. Moreover, the loss of HIF-2a in the lung could cause hypertension in the lungs of eHif2a-/ mice.
This antibody recognizes HIF-2alpha/EPAS1 DNA. It is derived from a 3-prime flanking area of the EPO gene. It is found in highly vascularized tissues like the lungs and the liver and may function as an artery-regulating regulator. It is expressed at a higher level in lung samples, placenta and villus segments of the placenta.
The immune system's production of Ag-specific Ab is controlled by the HIF gene. The anti-HIF-2-alpha/EPAS1 Marker can be used in the same way to test for these antibodies. The Anti-HIF-2-alpha/EPAS1 Marker at Boster Bio is a good choice for this research. The protein is extensively used in cancer immunotherapy, as well as for GC.
The HIF-2a/EPAS1 Protein is vital component of the immune system. The protein is crucial for increasing hapten-carrier immunity and controlling innate immunity. Furthermore, it is an essential component of pVHL. The protein also regulates function of Tfh-like T cells. The results show that anti-HIF-2alpha/EPAS1 Marker at Boster Bio can help in the discovery of new therapeutic targets.
PMID: 9000051 by Tian H., et al. Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells.
PMID: 9079689 by Hogenesch J.B., et al. Characterization of a subset of the basic-helix-loop-helix-PAS superfamily that interacts with components of the dioxin signaling pathway.
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