Transcription factor E2F6 Antibodies

Transcription factor E2F6 (E2F6)

Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a taste for the 5'-TTTCCCGC-3' E2F recognition website.

E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to control a subset of E2F-dependent genes whose products are necessary for entry into the cell cycle but not for normal cell cycle progression.

Gene Information

Human
Gene Name:	E2F6
Uniprot:	O75461
Entrez:	1876

Family

Belongs to:
E2F/DP family

Alternative Names

E2F transcription factor 6; E2F-6MGC111545; transcription factor E2F6

Molecular Weight

Mass (kDA):
31.844 kDA

Genomic Context

Human
Location:	2p25.1
Sequence:	2; NC_000002.12 (11444375..11466177, complement)

Tissue Specificity

Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.

Subcellular Localizations

Nucleus.

Diseases

• Neoplasms
• Retinoblastoma
• Malignant Neoplasms
• Carcinoma
• Hyperplasia
• Mammary Neoplasms
• Multiple Acyl Coenzyme A Dehydrogenase Deficiency
• Malignant Paraganglionic Neoplasm
• Malignant Neoplasm Of Breast
• Growth Arrest
• Osteosarcoma
• Cell Transformation, Neoplastic
• Lymphoma

• Cancer Of Nasopharynx
• Carcinogenesis
• Malignant Squamous Cell Neoplasm
• Perineurioma
• Nasopharyngeal Neoplasms
• Nerve Sheath Tumors
• Hypoxia

Interacting Proteins

• KRTAP10-7
• MAX
• TFDP1
• TFDP2

Pathways

• Cell Cycle
• Methylation
• Cell Proliferation
• Gene Silencing
• S Phase
• Dna Methylation
• Cell Cycle Arrest
• Cell Growth
• Translation
• Meiosis
• Rna Interference
• Regulation Of Cell Cycle
• Dna Repair

• Dna Replication
• Regulation Of Gene Expression
• Histone Modification
• Oxidative Phosphorylation
• Reverse Transcription
• Histone Methylation
• Chromatin Remodeling

PTMs

• Methylation
• Reduction
• Phosphorylation

• Acetylation
• Demethylation
• Cleavage

• Biotinylation

Research Areas

• Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/E2F6

Best Uses Of The E2F6 Marker From Boster Bio

Before we begin, let's briefly review some of the advantages and features of Boster bio. These benefits include high specificity and affinities, validation of antibodies on multiple platforms, and the ability to reward first reviewers for their efforts with product credits. Boster antibodies can be used worldwide by scientists. This article will cover some of the most popular applications for this marker. We'll also discuss applications and validation.

Boster Bio

Boster bio offers the E2F6 antibodies that can be used in studies to determine the role for this transcription factor. This high-affinity antibody has been tested in ChIP-chips and immunohistochemistry, as well as ELISA. Boster Bio has developed an antibody that recognizes RKO, Mouse and Human cell lines. It reacts with a variety of proteins and DNA sequences.

Specificity

We used a reporter gene test to confirm the E2F6 marker's specificity. For this, we used the same reporter gene as above but changed the sequence to amplify the E2F6 gene. To test the results, we used PCR to amplify the 3'UTRs for ELK1 AND circ–ITCH. The PCR products were cloned to reporter gene vectors by using polyclonal spots. Shanghai Yingbio Technology Co. Ltd. provided the reporter gene vectors.

Validation

The E2F6 gene plays a crucial role in DNA methylation. Several genes require E2f6 to be expressed. This marker binds to Stag3's promoter and Smc1b's. E2f6 is not methylated at the lysine neu position in germline cell cells. In addition, it is limited to germline genes.

The endogenous E2F6 associates with the polycomb proteins RYBP, Ring1, mph2, MEL-18, mph2, oncoprotein bmi1 and the polycomb group protein RYBP. These polycomb binding proteins suggest that E2F6 is linked to cancer. E2F6 is overexpressed in cells, which causes changes in cell growth parameters. This prevents cells from entering the S-phase and delays exit from G0 phase. This study also revealed that E2F6 is involved with transcriptional repressions of p16ink4a, p19arf.

To validate the E2F6 protein, an antibody that was specific for E2F6 (antibody specific for E2F6) was used. The results of IgG amplicon were compared with total chromatin input. CpG islands that are not specifically enriched were excluded from further analysis. Blue diamonds result from hybridization of an E2F6IP amplcon with Cy3or Cy5.

The E2F6 gene can be used to identify lung cancer cells. Its overexpression at LINC01436 has been shown to increase luciferase activity, and it has been used in the screening of cancer patients. Further, a gene expression test for E2F6 may help identify lung squamous cell carcinoma, adenocarcinoma, and other lung disorders.

Applications

The E2F6 gene is related to a number of other genes, including the TP53 family. It is involved in gene regulation and gene expression. In addition to cancer stem cells, E2Fs regulate NAD+ metabolism and promote tumor growth and invasion. E2F6 gene markers are important for cancer research. This study aims to determine if this gene is involved with the regulation of tumor stem cell growth.

This protein is also a powerful transcriptional inhibitor. It binds to DNA via its E2F recognized site and prefers the E2F recognition domain 5-TTTCCCGC-3. It does lack a transcriptional activation and pocket protein binding domains, but it does possess a modular suppression domain at its carboxyl terminal. Overexpression of the gene delays exit from S phase.

Studies have shown that E2F6 regulates a variety of genes and pathways. They play a crucial role in CSC self-renewal. It regulates the activity and transcription factors in CSCs. E2F6 is now used in more cancer research applications than ever. How do E2Fs function? How do they regulate gene activity?

E2F6 regulates the cell's cycle, limiting the number cells in the mid-late S phases. E2F-dependent gene expression of other genes also allows for the resumption/replication of replication. This protein has been shown to be a major driver in cancer initiation. In addition, it regulates the levels of various cellular proteins that contribute to gene expression. This could explain why some cancer cells are so aggressive.

E2F6 is reduced, which increases the speed of replication forks. A higher number of replicationforks results in a faster cell cycle, but less origin activity, which causes under-replication. E2F6 knockdown can be a powerful tool in cancer research. The E2F6 gene promotes the activation of replication forks in cancer cells, as well as the ability to achieve genomic recombination.