This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Protein Dr1.
Can bind to DNA by itself. Part of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
Human | |
---|---|
Gene Name: | DR1 |
Uniprot: | Q01658 |
Entrez: | 1810 |
Belongs to: |
---|
NC2 beta/DR1 family |
Down-regulator of transcription 1; down-regulator of transcription 1, TBP-binding (negative cofactor 2); NC2; NC2-BETA; Negative cofactor 2-beta; protein Dr1; TATA-binding protein-associated phosphoprotein
Mass (kDA):
19.444 kDA
Human | |
---|---|
Location: | 1p22.1 |
Sequence: | 1; NC_000001.11 (93345907..93369493) |
Nucleus.
Boster's DR1 marker is a reliable and specific primary antibody. This marker is extremely specific and widely employed in a variety of applications, including research. It is a fantastic option for research due to its accuracy and affordability. Here are a few of the most beneficial applications for DR1 markers:
As a leading provider of primary antibodies, Boster Bio offers a extensive range of reagents including ELISA kits, buffers, and lysates. These high-quality products can be used in a variety applications including WB, ELISA and FC. Boster Bio also offers rabbit polyclonal antibodies as well as a free secondary antibody when you purchase an initial antibody.
The reproducibility of data from antibodies is under constant scrutiny within the scientific community. PROGEN has launched a quality initiative to address the urgent demand for high-quality and reliable antibodies. This initiative is based on a comprehensive analysis of hundreds of thousands of published research articles about antibodies. Boster's high-quality initiative has received praise from many peer-reviewed journals.
Good primary antibodies must have positive and negative controls. Positive controls are lysates, Recombinant proteins, or genomic knockouts devoid of antigens. Negative controls, on the other hand could involve omitting the primary antibody to test for interference with secondary antibodies. Poor reproducibility can be caused by inadequate handling of primary antibodies. By following best practices when handling antibodies, sharing your results with colleagues, and implementing high-quality quality control procedures will ensure the reproducibility of your research.
A successful experiment requires top quality primary antibodies. Boster primary antibodies are made by highly skilled scientists with experience in monoclonal as well as polyclonal methods of antibody. Designed for Western Blotting, Rockland primary antibodies can be used for a range of research subjects. The company also provides primary antibodies that identify Akt Pathway, Apolipoproteins and Cell Signaling.
Boster Bio is a well-known manufacturer of high-quality ELISA kit, and also offers a wide range of quality detection and antibody kits for researchers and clinicians all over the world. These reagents are produced by Boster Bio in-house and are inspected for quality and are specifically designed for ELISA and WB applications. Their products are widely used across many fields, including neuroscience, immunology and other areas. They have earned the trust of more than 10,000 scientists worldwide.
Immunoprecipitation (IP) of antigens was carried out with 24 antibodies. Inputs were total proteins from flowers and the resulting IP was observed. In this image, the target band was marked by an arrow in black and silver staining was applied to the samples that were extracted. The samples were then cut and used in mass spectrometry analysis. The results were displayed in Figure 2.
Boster's antibodies are made from top-quality ingredients. They are safe for clinical and research use. They are manufactured using strict quality control processes and have an ISO-9001 certification. The company produces a broad range of antibodies, including rabbit anti-rat IgG (H+L) antibody, mAb-125-H, and anti-rabbit IgG.
Alper Biotech is another manufacturer of antibodies. They specialize in monoclonal aswell in polyclonal antibody production. Their antibodies bind with high affinity, specificity, and sensitivity, and are used in research in diagnostics, basic science, and clinical immunohistochemistry. Established in Denmark, Dako has over 1,000 products and is among the oldest companies for antibodies in the world. They also offer custom peptides and monoclonal antibodies as well as antibodies for flow cytometry.
Boster Biological Technology, Ltd., a biotechnology company located in Wuhan, China, produces high-quality antibodies and immunological reagents. Boster's selection of primary antibodies and ELISA kits are validated for IHC, WB, Flow Cytometry, and Western blot. Boster's process for manufacturing is quality-controlled. maintain high standards for primary antibodies as well as ELISA kits.
Secondary antibodies offer a broad range of sensitivities. Secondary antibodies identify the antigen by looking at its binding affinity as well as the degree to which it binds. The source of the primary antibody as well as its clonality determines the consistency of secondary antibodies. In addition to clonality, the sensitivity and specificity of primary antibodies differ based on the host species. In general, monoclonal antibodies are made from mice, rat or goat. However, this could be an issue of personal preference.
A Western Blot test could be used to determine the specificity of a primary antibody. This test requires a specific molecular size to exclude unspecific binding. In tissue sections, nonspecifically interacted proteins could bind antibodies. However a Western blot using an individual purified recombinant proteins may reveal an unspecific band. Additionally, a band with low affinity can be a sign of an immune response that is specific.
The primary antibodies are immunoglobulins that recognize an antigen in an immunocytochemical test. They are usually unconjugated however, they are able to be conjugated. The process of conjugation eliminates the necessity for secondary antibodies. In this way, the immunocytochemistry-related antibodies can be more specific than secondary antibodies. This makes primary antibodies among the most effective immunoglobulins for immunocytochemistry. The reaction's sensitivity and specificity is determined by the primary antibody's concentration.
Primary antibodies are immunoglobulins which are able to bind to specific antigens. Primary antibodies can be categorized by their affinity and specificity. A high affinity indicates a good antibody will only be able to bind to the antigen targeted. Insufficient specificity means that the antibody will bind to non-targeted proteins as well. They can be used to determine the specificity of primary antibody.
Flow cytometry is a technique to analyze particles and cells. There are numerous uses for it. Boster's antibodies, which are monoclonal or polyclonal, have high affinity for DR1 epitope, which allows them to recognize a variety of protein immunogens. Boster antibodies have been in use for over 25 years and have earned numerous citations.
The two kinds of antibodies are produced in a different method. For instance, monoclonal antibodies can be produced from DNA sequences, but most of the time they are made by the clones of plasma B cells that are identical to one other. Monoclonal antibodies do not require hybridoma cell lines. The cost of monoclonal antibody is lower. Monoclonal antibodies are also affordable to produce and don't require a lot of washing.
Monoclonal antibodies can also also be produced using hollow fibre systems and surface expanding tissue culture flasks. The cost is low, as compared to monoclonal antibodies made using hybridoma cell. The DR1 marker is also affordable. Cloning the DR1 gene in a lab could be a cost-effective option to obtain therapeutic antibodies.
Monoclonal antibodies are more sensitive to variations in the antigen and have an affinity that is high for the DR1 epitope. However, the polyclonal antibodies have more advantages in general research, like the production of antibodies and affinity purification. Additionally, they are easier to associate with antibodies that have labels. Monoclonal antibodies are more specific and are recommended for production with a high volume.
Boster's antibodies can be utilized to cheaply utilize this DR1 marker. However they should be prepared similarly to primary antibodies. Secondary antibodies should be aliquoted prior to storing at -20degC. Secondary antibodies are affected by repeated freezing and thawing which can reduce their staining capabilities. The antibodies should be stored in the refrigerator. For the most effective results, aliquoting the antibodies prior to use will ensure the best stability.
Recombinant proteins are most commonly produced by Escherichia coli. The yields of extracellular production can be as high as a gram per liter. In 1988 Functional Fab fragments were created in E. coli by secreting both V chains into periplasmic space. The oxidizing environment assists in the formation of disulfide bonds and the assembly is a functional Fv fraction.
PMID: 1339312 by Inostroza J.A., et al. Dr1, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription.
PMID: 8670811 by Goppelt A.R., et al. A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains.