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Facts about Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial.
The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed:29211711). A fraction of this 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is necessary for lysine succinylation of histones: partners with KAT2A on chromatin and supplies succinyl-CoA to histone succinyltransferase KAT2A (PubMed:29211711).
Human | |
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Gene Name: | DLST |
Uniprot: | P36957 |
Entrez: | 1743 |
Belongs to: |
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2-oxoacid dehydrogenase family |
2-oxoglutarate dehydrogenase complex component E2; dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutaratecomplex); Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenasecomplex; dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutaratedehydrogenase complex, mitochondrial; DLTS; E2K; EC 2.3.1; EC 2.3.1.61; OGDC-E2
Mass (kDA):
48.755 kDA
Human | |
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Location: | 14q24.3 |
Sequence: | 14; NC_000014.9 (74881913..74903743) |
Mitochondrion matrix. Nucleus. Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation.
This article will present the DLST Marker. It will also cover the history Steven Boster. Learn about High-affinity prim antibodies and optimization. There are many flow methods available. However, these guidelines will help to optimize your experiment. Boster Bio optimization guides and tips can help you decide which flow procedure to use. They will answer any questions you may have and provide a step by step guide to optimizing the results of your experiments.
The DLST Marker can be used to identify and mark different types of terminal blocks. Its adhesive qualities allow it to stick onto many surfaces. It can be found in India's major cities such as Mumbai, Delhi NCR (Bengaluru), Surat and Surat. To purchase the DLST marker, you need to visit the nearest Elmex store.
Steven Boster's story begins in 1993 when he first created his first product, an immunohistochemistry kits for humans. His innovations made Boster the largest catalog antibody producer in China. His innovative products as well as his trade secrets enabled the company develop high-sensitivity ELISA test kits for the laboratory. Steven Boster's history is rich with stories. We'll be exploring some of those stories in this article. Continue reading to learn about Steve Boster.
Steve Boster was born June 3, 1927 in Joliet (IL). He worked as a manager for many decades in the retail industry. He was also an active member of Concordia Hall in Staunton. Steve is survived, in addition to his parents. His siblings are Sandra Blanton, Jack Boster and Tammy. James Boster was also his brother.
Image-guided immunofluorescence (IHC) can be used to determine the presence or absence of two antigens on the same tissue section. In the latter case, only one antigen must be detected by the primary antibody, and the other must be recognized by the secondary antibody. In the ideal case, both antibodies should not cross react. This is possible due to the DLST marker which allows researchers to visualize multiple antibodies on the same tissue section.
The degree of antibody binding to the antigen determines the antibody's affinity. The equilibrium dissociation constant (KD) is used to measure affinity in bimolecular interactions. It is a reversible process, where the rate of binding is proportional to the concentration of both reactants and is equal to the rate of dissociation into their constituents. The reaction rate constants (Kp), of reactants can be used to determine the KD value. The antibody's affinity is higher if the KD value is lower.
Multiplex western blotting requires that antibodies be obtained from different species. Goat and sheep, for example, are distantly related. Multiplex western blotting also uses secondary antibodies. These secondary antibodies decrease antibody cross-reactivity and can lead to misinterpretations. The KD value, which is a measure the affinity of an antibody, is useful in determining the type gel that is required.
High-affinity prima antibodies are useful in analyzing the sample's immunoreactivity but not always for IHC. They can interfere with the ability to distinguish between antigens. Elution from the affinity column might require harsh or denatured methods. The end goal should be the antibody specificity and not the amount of antibodies detected.
Wuhan Boster Bio-engineering Co., Ltd. designed a detection tool based on Gal-3 INSTALL Hybridization. The samples were washed at 37°C. They were then incubated for one-hour. DAB was used to create the colours. Positive and negative signals were observed using controls. The detection kit was validated by detecting the presence or absence of Gal-3-labeled DNA.
PMID: 8268217 by Nakano K., et al. Human dihydrolipoamide succinyltransferase: cDNA cloning and localization on chromosome 14q24.2-q24.3.
PMID: 8076640 by Nakano K., et al. Isolation, characterization and structural organization of the gene and pseudogene for the dihydrolipoamide succinyltransferase component of the human 2-oxoglutarate dehydrogenase complex.