C-C motif chemokine 4 Antibodies

C-C motif chemokine 4 (CCL4)

Monokine with inflammatory and chemokinetic properties. Binds to CCR5.

One of the Significant HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

Gene Information

Human
Gene Name:	CCL4
Uniprot:	P13236
Entrez:	6351

Family

Belongs to:
intercrine beta (chemokine CC) family

Alternative Names

ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2

Molecular Weight

Mass (kDA):
10.212 kDA

Genomic Context

Human
Location:	17q12
Sequence:	17; NC_000017.11 (36103827..36105614)

Subcellular Localizations

Secreted.

Diseases

• Inflammation
• Cholangiocarcinoma
• Hiv Infections
• Toxic Effect Of Carbon Tetrachloride
• Malignant Neoplasms
• Neoplasms
• Infective Disorder
• Autoimmune Reaction
• Drug-induced Liver Injury
• Inflammatory Response
• Tissue Adhesions
• Immunologic Deficiency Syndromes

• Fibrosis
• Virus Diseases
• Injury To Liver
• Liver Cirrhosis
• Arthritis
• Liver Damage
• Liver Diseases

Interacting Proteins

• CCR5
• SLC30A2

Pathways

• Secretion
• Immune Response
• Pathogenesis
• Chemotaxis
• Chemokine Production
• Inflammatory Response
• Cell Migration
• Regeneration
• Cell Proliferation
• Cytokine Production
• Cell Activation
• Innate Immune Response
• Chemokine Secretion

• Cytokine Secretion
• Phagocytosis
• Angiogenesis
• Leukocyte Migration
• Cell Death
• Viral Replication
• Sensitization

PTMs

• Phosphorylation
• Cleavage
• Oxidation
• Acetylation
• Demethylation

• Carboxylation
• Reduction
• Phosphorothioation
• Citrullination
• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CCL4

Boster Bio: Best Uses Of The CCL4 Marker

This article will discuss the CCL4 marker and its mechanism of action and function. The clinical applications and the detection of CCL4 will be discussed. In addition, the presentation will also discuss the receptors and the mechanisms. In addition, you will be able to learn more about the CCL4 marker. Boster Bio offers comprehensive technical resources, blogs and information about diseases. The high-quality lysis buffers help you achieve good results.

Receptors

Boster Bio: The Best uses of the CCl4Marker is an effective tool that allows researchers and scientists to measure the levels of a particular protein in their samples. The CCL4 marker is a part of the chemokine group, and has numerous applications in immunology. It is frequently used in vaccines to determine the pathways that promote an effective response to the mRNA vaccine.

Mechanisms

The CCl4 marker, a cell-surface protein, can be found on all tissues' surfaces. It has been proven to activate the ER and induce Apoptosis. This article explains how CCl4 triggers ERK and causes apoptosis in various cell kinds. It also discusses the potential effects of CCl4 on cell signaling in different disease models.

The discovery of a brand new biomarker that is based on the expression of the CCL4 gene in lung cancer cells has implications for the diagnosis and treatment of this disease. The mechanisms that underlie CCL4 expression in a variety of cancers aren't fully understood but it is believed that CCL4 is responsible in stimulating TAM recruitment in lung cancer. The study is being carried out in mice.

The signaling pathway IL6-IL7 plays a crucial role in the growth of cancer cells. It is not an accurate indicator of a patient's prognosis. IL6 and CCL4 are two cytokines that promote macrophages to recruit via CCR5 which is a protein found that is present in macrophages. A recent study found that IL6 overexpression enhanced lung cancer cell metastasis, and that CCL4 expression was positively linked to the recruitment of M2 macrophages. Furthermore, CCL4 expression is related to the expression of SpiB the gene that is known to speed up the progression of cancer.

Moreover, CCL4 is highly correlated with Spi-B expression. SpiB can stimulate macrophage recruitment when it is overexpressed. This suggests that CCL4 and SpiB play an important role in the development of tumors. Therefore, it is essential to identify the specific mechanism by the way that CCL4 and Spi-B interact. This pathway should be studied when you observe that CCL4 expression is negatively connected to SpiB expression.

Clinical applications

The CCL4 marker is among the most significant markers of inflammation in a variety of human diseases. In fact it has been used to alter the systemic immune response in patients with acute coronary syndrome. Further research is needed to verify its effectiveness and determine its clinical use in the field of atherosclerosis. At present, this marker is mostly used in inflammation and inflammatory bowel diseases. It could also have clinical applications in a variety of atherosclerosis-related diseases.

There isn't a consensus on the cause of the non-synonymous CCL4 SNP However, a non-synonymous mutation in the gene has been associated with HIV infection susceptibility and progression of the disease. The QIAamp DNA blood kits were used to extract genomic DNA from blood samples that were whole. DNA was stored at 20°C. The CCL4 marker was identified by the DAPI technique.

The serum levels of CCL4 were also linked to levels of TC cholesterol and LDL cholesterol. The CCL4 antibodies also reduced plaque-related plaques by 28%. This suggests their potential application in treating atherosclerosis. These results indicate that CCL4 is vital in regulating specific immune responses. It has been found to be associated with lipid levels as well as inflammation-related Cytokines. These effects could be due to improved lipid profiles, a decrease in inflammation cytokines and inhibition of lymphocytes' recruitment.

The expression of CCL4 has a close relationship to other markers of cytotoxic T lymphocytes like CD8 and granzyme. It also is associated with FoxP3 and IL-10. Additionally, CCL4 expression has been found to be positively associated with the expression of IL-10. Further studies are needed to examine the connection between CCL4 and these markers. These findings could help to select the best treatment for patients with ESCC.

The detection

CCL4's gene has a low frequency polymorphism. However CCL4 is highly expressed in humans. It is responsible for the effects of a variety of inflammatory cytokines within the body. The effects are mediated through receptors on nicotinic acid-containing cells. These receptors are comprised of subunits A7 and A9.

Lung cancer is one of the most common cancers in China and has a low survival rate among Han Chinese people, mainly due to the delay in detection. CCL4 markers are often detected in tumor samples. This can aid in early diagnosis and better treatment. Chemokine (C-C motif ligand) 4 is a key chemokine that plays a role in tumor development and metastasis. Three SNPs, referred to as CCL4 polymorphisms (SNPs) have been found in patients with lung cancer.

CCL4 detection can be achieved with 96-well plates. The initial step of the test is coating rabbit CCL4 antibody onto the plate with 96 wells. Then, samples are pipetted into the wells. The immobilized antibody binds CCL4 in the samples. The HRP-conjugated strainavidin then pipetted into the wells. Then, TMB substrate solution is added. The dye will change from blue to yellow once the TMB substrate solution has been added. After this, the color intensity of the test is measured at 450 nm.

The CCL4 expression level correlates with several other genes, including Granzyme B, FoxP3, and IL-10. The mRNA for CCL4 and the other genes was extracted from 123 tumors of ESCC patients. The genes were analyzed using reverse transcriptase PCR (RT-PCR) and Spearman's Test to assess their relationship. Furthermore, the results show that CCL4 is a crucial marker in cancer cells.