Cytochrome P450 2U1 Antibodies

Cytochrome P450 2U1 (Cyp2u1)

Catalyzes the hydroxylation of arachidonic acid, docosahexaenoic acid and other long chain fatty acids. May regulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes (By similarity).

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Gene Information

Mouse
Gene Name:	Cyp2u1
Uniprot:	Q9CX98
Entrez:	71519

Family

Belongs to:
cytochrome P450 family

Alternative Names

cytochrome P450 2U1; cytochrome P450, family 2, subfamily U, polypeptide 1; EC 1.14.14.1; P450TEC

Molecular Weight

Mass (kDA):
60.578 kDA

Genomic Context

Mouse
Location:	3|3 G3
Sequence:	3;

Tissue Specificity

Widely expressed.

Diseases

• Malignant Neoplasms
• Malignant Neoplasm Of Breast
• Neoplasm Metastasis
• Neoplasms
• Muscle Spasticity
• Metastatic Malignant Neoplasm To The Lymph Node
• Non-neoplastic Disorder
• Nervous System Disorder
• Spastic Paraplegia, Hereditary
• Glioma
• Chloracne
• Carcinogenesis

• Epilepsy
• Patient Dependence On
• Paraplegia
• Neoplasms, Hormone-dependent
• Colorectal Neoplasms
• Spastic Paraplegia
• Medication Reaction

Pathways

• Transport
• Response To Retinoic Acid
• Cornification
• Keratinocyte Differentiation

PTMs

• Hydroxylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Cyp2u1

Best Uses Of The CYP2U1 Marker From Boster Bio

If you are a scientist, you are probably wondering what the best use of the CYP2U1 marker is. Boster can assist you in finding the right application or sample for you. There are many benefits to this product. It is accessible globally and widely used. You can submit results to species and applications. Third, product credits will be granted to you if your results are submitted. This program is only for scientists and it applies to all professionals and researchers around the globe.

Enhanced Chemiluminescence detection (ECL).

ECL is an imaging technology that uses antibodies that have been conjugated to HP. In the presence or enzyme, the antibodies bind with the chemiluminescent substrate to produce excited intermediates. These intermediates release strong blue emission at around 450 nm. The light emission occurs only during enzyme-substrate interactions. Once the substrate is exhausted, signal output ceases.

ECL is much more sensitive than conventional chemical chemiluminescence. The light emissions are captured with x-ray film, and then detected using a luminescent signal device, which has a higher sensitivity, resolution, dynamic range, and sensitivity. Researchers can also use cooled, CCD cameras to process images instantly without the need of a darkroom. However, most researchers prefer to capture the data on film. This method is more sensitive than Luminol's shorter half-life.

ECL can detect CYP2U1 from cell cultures. It is a sensitive test. The procedure requires a testis lysate that is diluted in 50 mM Tris pH 7.4 and 1% NP-40 (v/v). Before the ELISA was performed, the sample was treated with phosphatase inhibitor cocktails (protease inhibitor cocktails) and phosphatase inhibition cocktails. Thermo Scientific bought a Pierce BCA Protein Assay Kit. It was used for protein estimation. The gel was subjected to SDS-PAGE under reducing conditions. The membrane was then transferred for four hour.

Chemiluminescence detection has many advantages over other detection methods. They have a large dynamic range and a high ratio of signal-to noise. This makes it the preferred way to detect proteins in protein laboratories. It is also extremely sensitive, making it possible to detect a wide array of biological materials. This makes it a popular tool for Western blot experiments.

Enhanced Chemiluminescence – One of the most widely used methods for detecting cytochrome P450s. It is an excellent method to screen for pharmacological drugs. It is also used in animal models to monitor CYP2E1 levels. It has also been shown to be effective in detecting metabolites in cells and inducing oxidative stress in them.

The lnc-LFAR1 gene is a candidate for a molecular imaging tool. It is present in various cell types and stages of liver fibrosis. Enhanced Chemiluminescence Detection (ECL) of the CYP2U1 marker helps identify liver cancer by detecting CYP2U1 expression in cells.

Colorimetric detection

CYP2U1 is a gene that is expressed abundantly in the thymus and cerebellum. This gene is present in a variety of tissues, with lower levels. Interestingly, if a patient is high in CYP2U1, the disease could be related to lipid metabolism. CYP2U1 has been implicated in brain function, as well as metabolizing fatty acid.

Efficiency of protein transfer

Researchers now have a marker for CYP2U1 to help them understand how newly synthesized proteins are transported within the cell. CYP2U1 controls synaptic vesicle vesicle movement. When a neuron firing is initiated, synapses which are far from the soma do not influence the firing. Motor-driven transportation of newly synthesized amino acids favors the delivery resources to presynaptic targets. This distribution is less efficient than democratic distribution.