CHRNA3 Antibodies

Neuronal acetylcholine receptor subunit alpha-3 (CHRNA3)

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. .

Gene Information

Human
Gene Name:	CHRNA3
Uniprot:	P32297
Entrez:	1136

Family

Belongs to:
ligand-gated ion channel (TC 1.A.9) family

Alternative Names

cholinergic receptor, nicotinic, alpha 3; cholinergic receptor, nicotinic, alpha polypeptide 3; CHRNA3; LNCR2; MGC104879; NACHRA3; neuronal acetylcholine receptor subunit alpha-3; neuronal nicotinic acetylcholine receptor, alpha3 subunit; Nicotinic Acetylcholine R alpha 3; Nicotinic Acetylcholine Ra3; PAOD2

Molecular Weight

Mass (kDA):
57.48 kDA

Genomic Context

Human
Location:	15q25.1
Sequence:	15; NC_000015.10 (78593052..78620996, complement)

Subcellular Localizations

Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Diseases

• Malignant Neoplasms
• Nicotine Dependence
• Malignant Neoplasm Of Lung
• Tobacco Use Disorder
• Lung Neoplasms
• Chronic Obstructive Airway Disease
• Lung Diseases
• Lung Diseases, Obstructive
• Neoplasms
• Dysequilibrium Syndrome
• Non-small Cell Lung Carcinoma
• Adenocarcinoma

• Carcinoma
• Malignant Squamous Cell Neoplasm
• Pulmonary Emphysema
• Epilepsy
• Epilepsy, Frontal Lobe
• Cholangiocarcinoma

Pathways

• Pathogenesis
• Cell Proliferation
• Dna Methylation
• Localization
• Methylation
• Cell Adhesion
• Aging
• Response To Nicotine
• Chromosome Localization
• Habituation
• Prepulse Inhibition
• Catecholamine Secretion
• Dna Repair

• Cellular Response To Nicotine
• Demethylation
• Startle Response
• Response To Ethanol
• Sensitization
• Cell Growth
• Angiogenesis

PTMs

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CHRNA3

Boster Bio - Best Uses Of The CHRNA3 Marker

If you're looking for an Anti-Nicotinic Acetylcholine Receptor alpha/CHRNA3 Marker, then you've come to the right place. This article outlines some of the best uses of this highly specific marker. It also discusses how it compares to other antibodies, high-affinity primary antibodies, and recombinant human CHRNA3 protein.

Boster Bio

The CHRNA3 gene encodes a protein that responds to acetylcholine. It is located at postsynaptic membranes and cell junctions. Many biological assays detect CHRNB3 using monoclonal antibodies, which react with a wide variety of animal samples. Boster Bio uses rabbit and mouse as model organisms to develop its CHRNB3 antibodies. Once activated, the acetylcholine receptor beta-3 undergoes an extensive conformational change, opening up an ion-conducting channel across the plasma membrane.

Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 Marker

The CHRNA3 gene encodes the alpha 3 subunit of the nicotinic acetylcholine receptor. The nicotinic acetylcholine receptors are members of the superfamily of ligand-gated ion channels. These receptors function by mediating fast signal transmission across synapses. The receptors are composed of five homologous subunits.

These findings suggest that anti-Nicotinic acetylcholine receptor alpha 3 is a gene involved in the development of lung cancer. Several studies have linked these SNPs to the development of lung cancer. However, there are still no reliable methods to determine the exact causal link between CHRNA3 and lung cancer.

Molecular biology studies have shown that the CHRNA3 gene contains a single nucleotide polymorphism (SNP) in the CHRNA5 region. This substitution may have a functional impact on the receptor. A single nucleotide polymorphism (SNP) affecting the CHRNA3 gene could lead to the development of an anti-Nicotinic acetylcholine Receptor alpha 3/CHRNA3 marker.

The SNP a5SNP causes abnormalities in conducting airway epithelial cells and is associated with chronic pulmonary disorders. Repeated lesions of the epithelium are usually associated with impaired repair. Basal cells of the pulmonary epithelium function as progenitor cells and maintain tissue homeostasis. To detect this gene, lentiviral vectors are used to express the a5SNP and Green Fluorescent Protein in airway epithelial cells.

Uses of the CHRNA3 Marker

The CHRNA3 gene has been shown to be involved in the regulation of nicotine dependence and trait affective disposition. The CHRNA3 polymorphism, rs578776, has also been linked to the presence of a protective effect against smoking dependence. The CHRNA3 rs578776 genotype did not significantly impact baseline measures of depressed mood, smoking habit, and trait affective disposition. The findings suggest that the CHRNA3 gene may be useful as a diagnostic marker.

One of the most compelling uses of the CHRNA3 gene is to study the role of the gene in cognitive flexibility. Although multiple markers within a gene cluster are highly correlated, their effects on cognitive flexibility are so small that conservative multiple testing corrections are ineffective. Bonferroni's correction of a=0.05/(10*3)=0.002 is too stringent for this study because of the close correlation of the markers.

Several studies have suggested that minor alleles of the CHRNA3 gene are associated with the development of a weakened memory. The CHRNA5 gene-rs3841324 and rs6495307 have been found to decrease the likelihood of making persistent errors, but this association has not been confirmed in other studies. However, rs3841324 and rs286 9546 did have a significant association with non-perseverative errors.

The CHRNA3 gene polymorphism is associated with neuroticism in young Mexican adults. Other studies have also linked CHRNA3 with alcohol and nicotine dependence. Regardless of whether a person smokes or not, these SNPs can increase the risk of lung cancer. For these reasons, this gene polymorphism is of high relevance for health and addiction research. It has been proven to be a useful tool for assessing risk factors in smoking behavior.

High-affinity primary antibodies

Using the CHRNA3 marker to identify high-affinity primary antibodies offers an elegant approach to the study of antibody repertoires. Next-generation sequencing (NGS) has revolutionized antibody repertoire analysis by increasing sample depth and allowing for single-cell determination of the paired L and H chains. These analyses provide an invaluable genetic record of antibody clonal evolution. Using computational tools, researchers can reconstruct clonal lineages and understand antibody maturation.

The KD value of a specific antibody is determined by comparing the affinity of the antibody to its ligand. High affinity antibodies, also known as chimeric antigens (CAMs), have lower KD values than lower-affinity antibodies. The lower the KD value, the higher its affinity. The KD value is measured at UC Davis and reviewed by scientists at Abcam.

Primary antibodies recognize and bind to specific epitopes. Typically, antibodies are monoclonal or polyclonal. They are useful for detecting specific biomolecules and for measuring changes. GenScript's comprehensive collection of over 1000 high-affinity monoclonal antibodies covers all major areas of life sciences research and has been validated for multiple applications. Its highly specific and versatile antibodies allow scientists to study the function of certain genes, tissues, and cells.

The CHRNA3 gene is a good candidate for identifying antigens in a cell. However, because these markers are ubiquitous in human tissues, their detection is limited. It is essential to carefully select the primary antibodies and secondary reagents, as the latter has the ability to identify a specific target. If the primary antibody is specific for a cell marker, secondary antibodies must be specific for that marker.

To produce HLA-A*02:01, we generated two plasmids containing the peptide. The pET24a+ plasmid contains the HLA-A*02:01 protein, and we used a cloned IGFBPL1 peptide to produce inclusion bodies in E. coli. We grew the cells in autoinduction medium and then lysed them using 25 ml of bug-buster (Milipore Sigma) per litre of culture.