CETP Antibodies

Cholesteryl ester transfer protein (CETP)

Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, one of lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transfer of triglyceride from VLDL to HDL (PubMed:3600759, PubMed:24293641).

Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral cells and returned to the liver for elimination (PubMed:17237796). .

Gene Information

Human
Gene Name:	CETP
Uniprot:	P11597
Entrez:	1071

Family

Belongs to:
BPI/LBP/Plunc superfamily

Alternative Names

BPIFF; CETP; cholesteryl ester transfer protein; cholesteryl ester transfer protein, plasma; HDLCQ10; Lipid Transfer Protein I

Molecular Weight

Mass (kDA):
54.756 kDA

Genomic Context

Human
Location:	16q13
Sequence:	16; NC_000016.10 (56961950..56983845)

Tissue Specificity

Expressed by the liver and secreted in plasma.

Subcellular Localizations

Secreted, extracellular space. Secreted in plasma.

Diseases

• Atherosclerosis
• Cardiovascular Diseases
• Coronary Heart Disease
• Hypercholesterolemia
• Coronary Artery Disease
• Heart Diseases
• Diabetes Mellitus
• Arteriosclerosis
• Hyperlipidemia
• Dyslipidemias
• Diabetes Mellitus, Non-insulin-dependent
• Obesity
• Myocardial Infarction

• Infarction
• Hyperlipoproteinemias
• Insulin Resistance
• Inflammation
• Hyperalphalipoproteinemia (disorder)
• Hypertensive Disease
• Hyperlipoproteinemia Type Iib

Pathways

• Transport
• Cholesterol Transport
• Reverse Cholesterol Transport
• Cholesterol Efflux
• Secretion
• Cholesterol Esterification
• Excretion
• Pathogenesis
• Cholesterol Homeostasis
• Aging
• Lipid Transport
• Localization
• Lipid Binding

• Immune Response
• Menopause
• Glycosylation
• Response To Statin
• Coagulation
• Lipoprotein Transport
• Proteolysis

PTMs

• Oxidation
• Acetylation
• Glycosylation
• Cleavage
• Phosphorylation
• Reduction
• Acylation

• Biotinylation
• Deglycosylation
• Carboxylation
• Phosphorothioation
• Hydroxylation
• Iodination
• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CETP

Boster Bio: The Best Uses Of The CETP Marker

You can find more information about the CETP Marker here. This article will help you understand the CETP Marker, its benefits, and how to make the most of it. Learn how you can record your results using autoradiography films and how to remove primary or secondary antibodies from membrane. The guide also includes a brief description of membrane staining.

Optimum transfer efficiency by membrane staining

The best way to optimize your protein transport is membrane protein staining. Protein transfer is dependent on the type and amount of proteins present. Proteins with high molecular mass require high electric field, while proteins with low percentages need lower fields. Optimum transfer efficiency requires careful consideration of the buffer and membrane, which must be designed to promote quick transfer. Western blotting involves many variables that make it difficult to control and determine the transfer effectiveness. You can have greater confidence in your results by making the right decisions during the experimental design phase.

Membrane staing has been recognized as the gold standard for protein transfer. The factors that determine the transfer efficiency are many, including the amount and timing of gel presoaks, membrane choice, and the amount of methanol in the buffer. A molecular weight marker can be used to monitor the transfer efficiency. After transfer, membranes may be stained with Coomassie. Depending on what you need, you might also dry the membranes after transferring proteins. This method helps protein bind tightly to the membrane and prevents loss of signal during the transfer.

We tested the transfer efficiency of three different antibodies, IgG and A2M using both the dry and wet methods. We measured the amount left in the membrane by each method of protein transfer. We found that the transfer efficiency of three of these proteins was comparable or even higher than wet transfer. After each transfer method was completed, the gels had to be stained with a reversible marker. A gradient of 4% to 20 percent Tris-acetate gels can achieve the best membrane staining efficiency.

The pore size of the membrane plays an important role in the detection efficiency of a protein in WB. A membrane of 0.2 mm is better suited to proteins below 20kD. A larger membrane may be required for proteins with higher molecular masses, such CerP. For low-molecular-weight proteins, a membrane with an average pore of 0.2 mm is the best.

Benefits of using CETP Marker

The CETP Marker, an RNA sequence that determines if a person is at risk of developing heart disease or healthy ageing, is one of the most vital health markers. The CETP gene is responsible to transfer neutral lipids such as cholesteryl esters, and TAG. CETP activity elevates HDL C and LIPC modifies HDL. However, when CETP activity falls, plasma HDL -C is reduced and HDL remnants in circulation are removed through the renal clearing process and the liver holo-receptor.

Molecular studies showed that CETP was present in the airways and lung parenchyma of mice with inflammatory diseases. The protein's level was measured using PCR and a LightCyler 480 chromatography system. The PCR reaction was performed using a fluorescence labelled probe, an anchor prob, and a probe sensor. The results showed that CETP levels were 54°C in the wild type (V) and 61°C in mutant alleles (I).

The study also revealed a link between CETP gene levels and lipid levels. Low CETP levels were associated with higher HDL cholesterol levels, a lower chance of cardiovascular disease, and a longer life expectancy. While women had higher CETP levels that men, this effect was less in men. Despite its low incidence, the CETP genes is important for many aspects of human healthcare.

Because of its potential to increase HDL cholesterol, the CETP Marker is becoming more popular. CETP inhibition has also been shown reduce LDL, VLDL, LDL and other atherogenic lipoproteins. It is important to note, however, that it has not yet been studied for cardiovascular disease prevention. To determine if the CETP Marker works, you should use it in conjunction with other clinical testing.

Recording the test results using autoradiography film

Autoradiography uses radioactively labeled DNA to identify it in a sample. The film is composed of thin polyacrylamide gels which are dried using heat and thicker agarose gelatins which are transferred to the nylon membrane via capillary action. The film is then exposed by ethidiumbromide. This turns all the DNA black. The test results are usually recorded using the film. They are usually available within a week or so.

The film used in radiology is different from that used in autoradiography. The film's first exposure is "*", while the second exposure is called "C". The film's "C" color is due to the decomposition of 18lI. Because the exposures are different, corrections factors are calculated for each structure. To avoid this effect, the second must be longer than first.

How to remove primary and second antibodies from the membrane

We used Western blot to determine the best way to remove primary and secondary antibodies from the CEDP membrane. We modified the Western blot method slightly to compare samples with partially purified CETP. This was done by normalizing the mass of the protein present to the mass. The BSA-based bicinchoninic acid Protein Assay was used to determine protein concentration. The TP2 was kindly provided by Dr. Ruth McPherson. We also used a Bio-Rad set for deglycosylation tests.

This method targets CETPI, a newly discovered protein. CETPI is a part of the intestinal epithelium, and its secretion may function as a protective barrier against harmful effects of LPS in the circulation and Gram-negative bacteria. The CETP inhibitor torcetrapib, which was originally used to treat atherosclerosis in the past, was removed from advanced clinical trials due to side effects. Furthermore, it caused a higher ratio of infections in patients treated with the drug.