CCS Antibodies

Copper chaperone for superoxide dismutase (CCS)

Delivers copper to copper zinc superoxide dismutase (SOD1). .

Gene Information

Human
Gene Name:	CCS
Uniprot:	O14618
Entrez:	9973

Family

Belongs to:
No superfamily

Alternative Names

copper chaperone for superoxide dismutase; MGC138260; Superoxide dismutase copper chaperone

Molecular Weight

Mass (kDA):
29.041 kDA

Genomic Context

Human
Location:	11q13.2
Sequence:	11; NC_000011.10 (66593162..66606019)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Cytoplasm.

Diseases

• Angina Pectoris
• Coronary Artery Disease
• Malignant Neoplasms
• Coronary Heart Disease
• Myocardial Infarction
• Infarction
• Neoplasms
• Heart Diseases
• Stenosis
• Diabetes Mellitus
• Ischemia
• Pain
• Calcinosis

• Myocardial Ischemia
• Heart Failure
• Atherosclerosis
• Hypertensive Disease
• Sclerosis
• Cardiovascular Diseases
• Cerebrovascular Accident

Interacting Proteins

• SOD1

Pathways

• Cardiac Conduction
• Transport
• Localization
• Pathogenesis
• Angiogenesis
• Excretion
• Regeneration
• Secretion
• Defecation
• Cell Proliferation
• Aging
• Inflammatory Response
• Cell Cycle

• Cell Death
• Cell Growth
• Reflex
• Innervation
• Vasodilation
• Endocytosis
• Clathrin-mediated Endocytosis

PTMs

• Nitration
• Cleavage
• Oxidation
• Phosphorylation
• Ribosylation
• Ubiquitination

• Biotinylation
• Carboxylation
• Amidation
• Decarboxylation
• Acylation
• Reduction

• Farnesylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CCS

Best Uses Of The CCS Marker

Anti-CD68 (Macrophage Marker) Monoclonal Antibody from Boster Bio is designed for research using this protein. Boster scientists are able to submit results in specific studies specific to species or applications, and also receive credit for the product. These benefits are available to scientists from anywhere around the globe. This informative article will provide additional information about the antibody for researchers who are interested. We also discuss ways to use it to identify human immune cells.

Boster Bio Anti-CD68 (Macrophage Marker) Monoclonal Antibody

CD68 which is activated by inflammatory stimuli, is an Scavenger-receptor, and is elevated in macrophages. It binds to oxidized LDL, phosphatidylserine and can move between plasma membranes and endosomes. Studies have demonstrated that CD68 binding and the internalization of oxLDL is linked to atherogenesis.

Boster Bio Anti CD68 (Macrosialin), Monoclonal Autoantibody reacts to Human CD68 protein. Boster Bio Anti-CD68 Marker Monoclonal Antibody has no known hazards and has 0.05 percent Thimerosal. It has been validated for IHC, WB, and immunohistochemistry.

CD68 is a transmembrane -protein that is expressed on a variety of human cells. It plays an important role in the phagocytic activity of macrophages in tissues, extracellular cell-cell interactions, and intracellular metabolic lysosomal processes. Rapid recirculation could allow macrophages to move over substrates that contain selectins.

CD68 is an enzyme that binds to various proteins and is expressed by a wide range of cells. It is found most often in monocytes of the human body and tissue microphages. However it can also be found in tumor stroma. It has been found in various non-hemoid tumor cell lines, granulocyte deficient states and inflammatory bowel disease.

This Monoclonal Antibody recognizes the CD68 protein found on macrophages as well as histiocytes. It can be useful in the diagnosis of diseases involving abnormal macrophages, including Gaucher's disease as well as histiocytic lymphoma. These antibodies are sold through biopharmaceutical companies worldwide.

The anti-CD68 mAb was tested by flow cytometry and immunohistochemistry. It was used to analyze the expression of CD68 within synovial membranes and skin fibroblasts. The application of this test in the diagnosis and treatment of osteoarthritis, rheumatoid arthritis, and other diseases is pending however, it is certainly worth noting.

This antibody is available in a variety of sizes, Alexa Fluor dyes, and conventional FITC/PE or AP-Cytometry membranes. Numerous techniques have been devised to detect CD68 in macrophage membranes including flow cytometry. For intracellular detection in flow cytometry, it is essential to permeate for example, with Bio-Rad's LeucopermTM BUF09 and for IHC, citrate buffer is recommended.

CD68 is a membrane-bound glycoprotein which has a molecular mass of 110 kDa. It is a member of the LAMP family and plays a significant role in the process of phagocytosis. The protein is usually found in the lysosome, but it can circulate on the cell surface. It is among the first indicators of inflammation.

A secondary goat anti-antibody was made in Wuhan, China, and was diluted 1:500 with PBS/BSA. Slides were then incubated with the SABC complex for 30 min. The reaction was developed with an chromogen solution that contained 0.03 percent hydrogen peroxide and the intensity was analyzed with a light microscope. Following the development of the secondary antibody the specimens were washed using PBS/BSA three times, counterstained with Harris Hematoxylin for 20 second, and mounted in Permount resin.

CD68 is a marker for macrophages and also binds to lectins in the kidney. This allows macrophage subsets be directed to specific organs or tissues. Furthermore, CD68 is rapidly recirculated from lysosomes and endosomes. Therefore, this monoclonal antibody is effective in detecting macrophages in various tissues and organs.

In the Life Science industry, reproducibility is a critical issue. Abcam is working to solve this issue by introducing recombinant monoclonal antibody and knockout edited cells lines. These products are optimized for gold-standard validation. They assist researchers in identifying the source of their data with greater accuracy. This antibody allows you to confirm the validity of your results.

The peptide antibodies were created by immunization with synthetic peptides and purified IgG. These antibodies were tested against macrophages in culture and confirmed by FACS analysis. In addition, the monoclonal antibody was able to stain the macrophages of the culture. To determine the most effective levels of dilutions, a titration using a checker of the monoclonal antibody was conducted.

Similarly, Gal1 has a significant impact on tumor microenvironment and response to treatment. Gal1 is neutralized within the tumor microenvironment. This results in an altered response to treatment from tumor cells. Additionally, the knockdown of Gal1 in tumor cells lowers the amount of myeloid cell within tumors. Gal1 has been demonstrated to inhibit myeloid-derived suppressor cells and macrophages with CD68+.

In addition, Boster Bio Anti-CD68 (Mactophage Marker) Monoclonal Protein is an excellent complement to other antibodies for monitoring inflammation. Your clinician will be able better treat patients since it is able to distinguish between inflammation cells and Apoptotic ones. There are several ways you can test for this antibody. The best method is to order it online from a reputable source.

The monoclonal antibody for CD68 is highly specific and a powerful instrument for studying the function of the CD68 gene. This protein is crucial for the transformation of monocyte-derived macrophages to foam cells. Dasatinib is a promising therapy for ORC as it improves autophagy impairment and inhibits the process of macrophage infiltration and lipogenesis.

For this study, monocytes were collected randomly from a blood center located in Wuhan, P.R. China, and plated in six-well culture plates. After two hours of equilibration PBS was added as an inhibitor. After the cells had been collected, they were stained with a dual-color antibody panel comprised of CD14 and TLR-4. The samples were then analyzed using a six-fluorescent-parameter BD LSRII. A one-way ANOVA was used to determine the statistical significance. A p-value of 0.05 was considered statistically significant.