CCR10 Antibodies

C-C chemokine receptor type 10 (CCR10)

Receptor for chemokines SCYA27 and SCYA28.

Subsequently transduces a signal by increasing the intracellular calcium ions level and stimulates chemotaxis at a pre-B line.

.

Gene Information

Human
Gene Name:	CCR10
Uniprot:	P46092
Entrez:	2826

Family

Belongs to:
G-protein coupled receptor 1 family

Alternative Names

CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2

Molecular Weight

Mass (kDA):
38.416 kDA

Genomic Context

Human
Location:	17q21.2
Sequence:	17; NC_000017.11 (42678889..42681843, complement)

Tissue Specificity

Expressed at high levels in adult testis, small intestine, fetal lung, fetal kidney. Weaker expression was observed in many other adult tissues including spleen, thymus, lymph node, Peyer patches, colon, heart, ovary, peripheral blood lymphocytes, thyroid and spinal cord. Also expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells. Also detected in T-cells and in skin-derived Langerhans cells.

Subcellular Localizations

Cell membrane; Multi-pass membrane protein.

Diseases

• Inflammation
• Cholangiocarcinoma
• Dermatitis
• Neoplasms
• Tissue Adhesions
• Skin Neoplasms
• Neoplasm Metastasis
• Dermatologic Disorders
• Dermatitis, Atopic
• Malignant Neoplasms
• Melanoma
• Lymphoma
• Malignant Paraganglionic Neoplasm

• Lymphoma, T-cell, Cutaneous
• T-cell Lymphoma
• Psoriasis
• Dermatitis, Allergic Contact
• Mycoses
• Mycosis Fungoides
• Contact Dermatitis

Interacting Proteins

• ANXA2
• S100A10

Pathways

• Pathogenesis
• Localization
• Chemotaxis
• Secretion
• Cell Adhesion
• Immune Response
• Hypersensitivity
• Angiogenesis
• Cell Proliferation
• Lactation
• Regeneration
• Wound Healing
• Lymphocyte Migration

• Cytokine Production
• Cell Migration
• Tropism
• Cell Motility
• Cytokine Secretion
• Inflammatory Response
• Leukocyte Migration

PTMs

• Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CCR10

Boster Bio: Best Uses Of The CCR10 Marker

Steven Boster has been a biotech entrepreneur since the 1990s and is a pioneer in antigen detection. His colleagues call him "the guy who converts science into the lavatory". His innovative methods have made Boster a global star. He has been responsible for creating many IHC products, including hundreds if primary antibodies. His company became the largest Chinese catalog antibody business in the late 1990s. Boster developed and commercialized a proprietary ELISA platform called PicoKine (tm), which uses trade secrets to deliver high sensitivity ELISA tests.

Current research on CCR10

G-protein-coupled CC chemokine receptor CCR10 (also called GPR-2) is expressed in a subset of T memory cell, IgA-secreting cells and EBV-immortalized cells. CCR10 and its receptors regulate migration and homing between T and IgA-secreting cell lines.

Four GPCR-associated genes have been linked to various forms of human carcinogenesis. RTPCR and Western blotting validation showed that CCR10 expression was found in hepatocytes derived from human HCC tumors and paracancerous liver samples. FACS analysis showed that HCC-related hepatocytes expressed CCR10 at significantly higher levels than normal hepatocytes.

Interestingly, knocking out CCR10 did not significantly affect the expression of PI3K or Akt. However, knockout mice with CCR10 deletion exhibited significantly higher tumor growth than control mice, suggesting that the gene may be important for long-term inflammation-induced hepatocarcinogenesis. The findings also indicate that hepatic inflammation is associated with elevated CCR10 expression. Further, knocking-out mice expressing CCR10 did not significantly reduce the expression of PI3K or Akt.

Activated VEGF-10 promotes expression in a variety tissue types of VEGF/C/D (MMP-2/9) and TIMP-1/2. CCR10 activation promotes the expression VEGF-C/D, MMP-2/9, and TIMP-1/2, a signal path that regulates tumor growth. These studies show that CCR10/CCL27 are interconnected. However, there are few studies that have investigated the downstream signal pathways in cancer cells.

However, this does not rule out the possibility that CCR10 may have a role in other aspects of B cell differentiation. CCR10 may regulate the expression of integrins such as a4b7. This gene is essential for normal immune function. CCR10/CCR9 research is ongoing. The future of this gene's function looks exciting.

Recent research has shown that CCR10 is an oncogene important in many malignancies. However, the exact role of this gene for tumor growth is not known. Although CCR10 is an important cancer oncogene, it has not been extensively studied in patients with cancer. Researchers have looked at CCR10 expression and CCL27 ligand in human NSCLC cell strains. Blockage of CCR10 caused cell multiplication to be inhibited in h257 and A549 cancer cells.

Potential clinical applications

Humanized mice that have been genetically engineered to express the CCR10 marker are protected against pulmonary fibrosis in a similar manner to nonhumanized NSG mice. After intravenous injections with IPF cells, humanized NSG animals were given either 5 mg/kg of KB004 (or an isotype control KB243) as an injectable drug. CCR10+ cell numbers in humanized NSG mice's lungs were significantly higher than in nonhumanized mice as determined by hydroxyproline staining.

CCR10+ cells in normal lung tissue are very rare, but they were found in rapid-IPF lungs biopsies. CCL28 levels were associated with poor progression free survival and poor SAP scores in IPF patients. CCR10+ cell are a novel target in lung fibrosis. CCR10's clinical value as a biomarker in lung tissue analyses is currently unknown.

A KO Cas9-mediated CCR10 knockdown reduces the expression of MPC and premature senescence in fibroblasts progeny. CCR10-KO cells were generated by transducing four different guideRNAs. These RNAs were used in the creation of normal and IPF-fibroblast cultures. 294 bp PCR product bands were obtained. Nontargeting control groups were measured at 1325 BC. For validation, combinations 1 through 4 were selected.

The CCR10 marker is not only useful in immunology but also has potential clinical applications. It is useful in predicting cancer development by identifying risk factors. It helps to determine the severity and cause of an underlying condition. Researchers found that CCR10-positive CLA+ cell lines were associated with higher rates for leukemia and other cancers in one study. CCR10-positive CLA+ cells are also associated with a higher risk of gliomagenesis.

Moreover, CCR10+ cells were consistently higher in IPF lung samples. CCR10-positive cells also coexpress EphA3. These cells were also found in high numbers in IPF fibroblasts and significantly more prevalent in IPF fibroblasts than in control patients. CCR10+EphA3+ was also common in IPF fibroblasts, although it was rare in normal lungs.

Biography

One of the most important functions of the CCR10 gene is the regulation of epithelial immunity. Understanding the biology of CCR10 is critical to understanding how this gene works. This seven-transmembrane transmembrane domain G protein protein interacts well with the chemokine CCL27. It is abundantly expressed throughout the epidermis. CCR10 expression in melanomas is strongly correlated with their ability to spread. This antibody is for research use only and not for clinical diagnosis. Primary Antibodies are protected for one year after receipt.